Different p53 mutation patterns in colorectal tumors from smokers and nonsmokers

Chi Chou Huang, Ya Wen Cheng, Meng Cheng Chen, Yu Shyang Lin, Ming Chih Chou, Huei Lee

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Epidemiological studies consistently find associations between colorectal cancer and cigarette smoking; however, there are little molecular data supporting the association. To examine the relationship between cigarette smoking and colorectal cancer, we compared p53 mutation patterns in colorectal tumors from smokers and nonsmokers. In this study, 153 tumor tissues from colorectal cancer patients, including 63 smokers and 90 nonsmokers, were examined for p53 mutation and p53 protein expression by direct sequencing and immunohistochemistry (IHC), respectively. p53 mutations were detected in 77 of 153 (50.3%) colorectal tumors, and no difference was observed in the p53 mutation frequencies in tumors from smokers and nonsmokers (33 of 63, 50.8% for smokers vs. 44 of 90, 48.9% for non-smokers, P = 0.743). IHC showed that p53-immunoreactive tumors were positively correlated with p53-mutated tumors (P <0.0001). G:C→A:T transition and G:C→T:A transversion were the predominant types of mutations detected in the tumor p53 genes. G:C→A:T mutation was relatively more common in nonsmokers than in smokers (93.5% for non-smokers vs. 77.3% for smokers), although this difference was not significant. The frequency of deletion mutation in smoker tumors, however, was significantly higher than that in nonsmoker tumors (7 of 33, 21.2% for smokers vs. 1 of 44, 2.3% for non-smokers, P = 0.01). Although there were only a few cases of p53 deletion mutation in this study, the observation of a higher frequency of p53 deletion mutation in smoker tumors supports the association between cigarette smoking and the development of colorectal cancer.

Original languageEnglish
Pages (from-to)527-532
Number of pages6
JournalEnvironmental and Molecular Mutagenesis
Volume47
Issue number7
DOIs
Publication statusPublished - Aug 2006
Externally publishedYes

Fingerprint

tumor
Tumors
Colorectal Neoplasms
mutation
Mutation
Sequence Deletion
Neoplasms
cancer
smoking
Tobacco Products
Smoking
Immunohistochemistry
p53 Genes
Mutation Rate
Epidemiologic Studies
Observation
Genes
Tissue
protein
gene

Keywords

  • Cigarette smoking
  • Colorectal cancer
  • p53 mutation patterns

ASJC Scopus subject areas

  • Environmental Science(all)
  • Environmental Chemistry
  • Genetics
  • Genetics(clinical)
  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Huang, C. C., Cheng, Y. W., Chen, M. C., Lin, Y. S., Chou, M. C., & Lee, H. (2006). Different p53 mutation patterns in colorectal tumors from smokers and nonsmokers. Environmental and Molecular Mutagenesis, 47(7), 527-532. https://doi.org/10.1002/em.20222

Different p53 mutation patterns in colorectal tumors from smokers and nonsmokers. / Huang, Chi Chou; Cheng, Ya Wen; Chen, Meng Cheng; Lin, Yu Shyang; Chou, Ming Chih; Lee, Huei.

In: Environmental and Molecular Mutagenesis, Vol. 47, No. 7, 08.2006, p. 527-532.

Research output: Contribution to journalArticle

Huang, Chi Chou ; Cheng, Ya Wen ; Chen, Meng Cheng ; Lin, Yu Shyang ; Chou, Ming Chih ; Lee, Huei. / Different p53 mutation patterns in colorectal tumors from smokers and nonsmokers. In: Environmental and Molecular Mutagenesis. 2006 ; Vol. 47, No. 7. pp. 527-532.
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abstract = "Epidemiological studies consistently find associations between colorectal cancer and cigarette smoking; however, there are little molecular data supporting the association. To examine the relationship between cigarette smoking and colorectal cancer, we compared p53 mutation patterns in colorectal tumors from smokers and nonsmokers. In this study, 153 tumor tissues from colorectal cancer patients, including 63 smokers and 90 nonsmokers, were examined for p53 mutation and p53 protein expression by direct sequencing and immunohistochemistry (IHC), respectively. p53 mutations were detected in 77 of 153 (50.3{\%}) colorectal tumors, and no difference was observed in the p53 mutation frequencies in tumors from smokers and nonsmokers (33 of 63, 50.8{\%} for smokers vs. 44 of 90, 48.9{\%} for non-smokers, P = 0.743). IHC showed that p53-immunoreactive tumors were positively correlated with p53-mutated tumors (P <0.0001). G:C→A:T transition and G:C→T:A transversion were the predominant types of mutations detected in the tumor p53 genes. G:C→A:T mutation was relatively more common in nonsmokers than in smokers (93.5{\%} for non-smokers vs. 77.3{\%} for smokers), although this difference was not significant. The frequency of deletion mutation in smoker tumors, however, was significantly higher than that in nonsmoker tumors (7 of 33, 21.2{\%} for smokers vs. 1 of 44, 2.3{\%} for non-smokers, P = 0.01). Although there were only a few cases of p53 deletion mutation in this study, the observation of a higher frequency of p53 deletion mutation in smoker tumors supports the association between cigarette smoking and the development of colorectal cancer.",
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