Dietary oxidized frying oil enhances tissue α-tocopherol depletion and radioisotope tracer excretion in vitamin E-deficient rats

Jen Fang Liu, Ching Jang Huang

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Rats fed a diet containing 15% oxidized frying soybean oil (OFO) have been shown to have significantly lower tissue α-tocopherol (α-T) concentration than rats fed a 15% fresh soybean oil diet. To examine the turnover of α-tocopherol, a depletion-repletion experiment and a radioisotope tracer study were conducted. Two groups of male weanling Long- Evans rats were fed vitamin E-deficient diets containing either 15% OFO or 15% vitamin E-stripped fresh soybean oil (control). After 9 wk of depletion, rats fed the OFO diet had significantly higher plasma pyruvate kinase (PK) activity and lower concentrations of α-T in RBC, adrenal gland, heart, kidney, liver, spleen, testis and muscle compared with controls (P <0.05), indicating that the vitamin E-deficient status was aggravated by feeding the OFO diet. After 12 wk, the depleted rats were intraperitoneally injected with a dose of all-rac-α-T (2.5 mg/rat, dissolved in Vitamin E-stripped corn oil) every other day. Three doses were administered to each rat during the 1-wk repletion period. Plasma PK activity decreased in both groups (P <0.05) after repletion but that of the OFO rats was still significantly higher than that of the control group. The repleted OFO group also had significantly lower α-T concentration in adrenal gland, epididymal fat, liver and spleen than the repleted control group. Two rats from each group that had been vitamin E-depleted for 16 wk were injected intraperitoneally with a single dose of 5-methyl-14C-RRR-α-T (740 kBq/kg body weight). During the week after dosing, the radioactivity excreted in urine and feces of the OFO group was 1.3-and 1.7-fold, respectively, that of the control group. Tissue retention of radioactivity was also lower in the OFO rats than in the control rats. The results suggest that more of the α-T in the body was catabolized or turned over in rats fed the OFO-containing diet.

Original languageEnglish
Pages (from-to)2227-2235
Number of pages9
JournalJournal of Nutrition
Volume126
Issue number9
Publication statusPublished - Sep 1996

Fingerprint

radiolabeling
frying oil
Soybean Oil
Tocopherols
Vitamin E
tocopherols
Radioisotopes
soybean oil
vitamin E
Oils
excretion
rats
Diet
repletion
diet
Pyruvate Kinase
pyruvate kinase
Adrenal Glands
adrenal glands
Radioactivity

Keywords

  • depletion
  • oxidized frying oil
  • radioisotope tracer
  • rats
  • vitamin E

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Food Science

Cite this

Dietary oxidized frying oil enhances tissue α-tocopherol depletion and radioisotope tracer excretion in vitamin E-deficient rats. / Liu, Jen Fang; Huang, Ching Jang.

In: Journal of Nutrition, Vol. 126, No. 9, 09.1996, p. 2227-2235.

Research output: Contribution to journalArticle

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