Abstract
Glutamine (Gln) has been demonstrated to have benefit in the modulation of systemic immunity in sepsis. However, the effects of Gln on local immunity and intra-lymphocyte cytokine expression have not been investigated in mice with gut-derived sepsis. This study evaluated the influence of a Gln-enriched diet on interleukin (IL)-6 expression in organs and Th1/Th2 type cytokine production within lymphocytes in septic mice. Male ICR mice were assigned to control and Gln groups. The control group was fed a semi-purified diet, while in the Gln group, Gln replaced part of the casein. After feeding the respective diets for 3 weeks, sepsis was induced by cecal ligation and puncture (CLP). Mice were sacrificed at 0, 6, 12 and 24 h after CLP and their organs were harvested for further analysis. Results showed that IL-6 levels in the liver were decreased, whereas levels were increased in the lungs, kidneys and intestines with the progression of sepsis in both groups. Also, intra-lymphocyte interferon (IFN)-γ expression decreased and IL-4 expression increased during sepsis. Compared to the control group, the Gln group had higher levels of IL-6 in the liver and lower levels in other organs at various time points. Lymphocyte IFN-γ expression in the Gln group was higher, and IL-4 levels were lower than those of the control group after CLP. These results suggest that Gln supplementation decreased IL-6 production in non-hepatic organs, while reducing intra-lymphocyte IL-4 and enhancing IFN-γ expressions. This change may reverse the Th2 type response to a more-balanced Th1/Th2 response during sepsis.
| Original language | English |
|---|---|
| Pages (from-to) | 329-334 |
| Number of pages | 6 |
| Journal | Cytokine |
| Volume | 31 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - Sep 7 2005 |
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Keywords
- Glutamine
- Interferon-γ
- Interleukin-4
- Interleukin-6
- Sepsis
ASJC Scopus subject areas
- Endocrinology
- Molecular Biology
- Immunology
- Immunology and Allergy
Cite this
Dietary glutamine supplementation modulates Th1/Th2 cytokine and interleukin-6 expressions in septic mice. / Yeh, Chiu Li; Hsu, Chun Sun; Yeh, Sung Ling; Chen, Wei Jao.
In: Cytokine, Vol. 31, No. 5, 07.09.2005, p. 329-334.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Dietary glutamine supplementation modulates Th1/Th2 cytokine and interleukin-6 expressions in septic mice
AU - Yeh, Chiu Li
AU - Hsu, Chun Sun
AU - Yeh, Sung Ling
AU - Chen, Wei Jao
PY - 2005/9/7
Y1 - 2005/9/7
N2 - Glutamine (Gln) has been demonstrated to have benefit in the modulation of systemic immunity in sepsis. However, the effects of Gln on local immunity and intra-lymphocyte cytokine expression have not been investigated in mice with gut-derived sepsis. This study evaluated the influence of a Gln-enriched diet on interleukin (IL)-6 expression in organs and Th1/Th2 type cytokine production within lymphocytes in septic mice. Male ICR mice were assigned to control and Gln groups. The control group was fed a semi-purified diet, while in the Gln group, Gln replaced part of the casein. After feeding the respective diets for 3 weeks, sepsis was induced by cecal ligation and puncture (CLP). Mice were sacrificed at 0, 6, 12 and 24 h after CLP and their organs were harvested for further analysis. Results showed that IL-6 levels in the liver were decreased, whereas levels were increased in the lungs, kidneys and intestines with the progression of sepsis in both groups. Also, intra-lymphocyte interferon (IFN)-γ expression decreased and IL-4 expression increased during sepsis. Compared to the control group, the Gln group had higher levels of IL-6 in the liver and lower levels in other organs at various time points. Lymphocyte IFN-γ expression in the Gln group was higher, and IL-4 levels were lower than those of the control group after CLP. These results suggest that Gln supplementation decreased IL-6 production in non-hepatic organs, while reducing intra-lymphocyte IL-4 and enhancing IFN-γ expressions. This change may reverse the Th2 type response to a more-balanced Th1/Th2 response during sepsis.
AB - Glutamine (Gln) has been demonstrated to have benefit in the modulation of systemic immunity in sepsis. However, the effects of Gln on local immunity and intra-lymphocyte cytokine expression have not been investigated in mice with gut-derived sepsis. This study evaluated the influence of a Gln-enriched diet on interleukin (IL)-6 expression in organs and Th1/Th2 type cytokine production within lymphocytes in septic mice. Male ICR mice were assigned to control and Gln groups. The control group was fed a semi-purified diet, while in the Gln group, Gln replaced part of the casein. After feeding the respective diets for 3 weeks, sepsis was induced by cecal ligation and puncture (CLP). Mice were sacrificed at 0, 6, 12 and 24 h after CLP and their organs were harvested for further analysis. Results showed that IL-6 levels in the liver were decreased, whereas levels were increased in the lungs, kidneys and intestines with the progression of sepsis in both groups. Also, intra-lymphocyte interferon (IFN)-γ expression decreased and IL-4 expression increased during sepsis. Compared to the control group, the Gln group had higher levels of IL-6 in the liver and lower levels in other organs at various time points. Lymphocyte IFN-γ expression in the Gln group was higher, and IL-4 levels were lower than those of the control group after CLP. These results suggest that Gln supplementation decreased IL-6 production in non-hepatic organs, while reducing intra-lymphocyte IL-4 and enhancing IFN-γ expressions. This change may reverse the Th2 type response to a more-balanced Th1/Th2 response during sepsis.
KW - Glutamine
KW - Interferon-γ
KW - Interleukin-4
KW - Interleukin-6
KW - Sepsis
UR - http://www.scopus.com/inward/record.url?scp=23944432915&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=23944432915&partnerID=8YFLogxK
U2 - 10.1016/j.cyto.2005.06.001
DO - 10.1016/j.cyto.2005.06.001
M3 - Article
C2 - 16026997
AN - SCOPUS:23944432915
VL - 31
SP - 329
EP - 334
JO - Cytokine
JF - Cytokine
SN - 1043-4666
IS - 5
ER -