Diagnosis of respiratory tract viruses in 24 h by immunofluorescent staining of shell vial cultures containing Madin-Darby Canine Kidney (MDCK) cells

Shin Ru Shih, Kuo Chien Tsao, Hsiao Chen Ning, Yhu Chering Huang, Tzou Yen Lin

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Nine hundred and seventy-eight clinical specimens were examined taken from patients with respiratory tract viruses (RV)-like syndrome between November 1996 and July 1998. The study was undertaken to evaluate the effectiveness of centrifuge-enhanced shell vial cultures (SVC) containing Madin-Darby Canine Kidney (MDCK) cells, combined with immunofluorescent (IF) staining in 24 h. This technique rapidly detects and identifies respiratory tract viruses. The conventional tube culture system with multiple cell lines would ordinarily detect RV within 3-30 days. The SVC/IF method using single cell line (MDCK cells) allowed detection of 81.5% of influenza A virus, 72% of parainfluenza virus, 82.6% of respiratory syncytial virus (RSV) and 79.6% of adenovirus in 24 h. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)77-81
Number of pages5
JournalJournal of Virological Methods
Volume81
Issue number1-2
DOIs
Publication statusPublished - Aug 1999
Externally publishedYes

Fingerprint

Madin Darby Canine Kidney Cells
Respiratory System
Staining and Labeling
Viruses
Paramyxoviridae Infections
Cell Line
Respiratory Syncytial Viruses
Influenza A virus
Adenoviridae

Keywords

  • MDCK cells
  • Respiratory viruses
  • Shell vial culture

ASJC Scopus subject areas

  • Virology

Cite this

Diagnosis of respiratory tract viruses in 24 h by immunofluorescent staining of shell vial cultures containing Madin-Darby Canine Kidney (MDCK) cells. / Shih, Shin Ru; Tsao, Kuo Chien; Ning, Hsiao Chen; Huang, Yhu Chering; Lin, Tzou Yen.

In: Journal of Virological Methods, Vol. 81, No. 1-2, 08.1999, p. 77-81.

Research output: Contribution to journalArticle

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