Diacylglycerol formation induced by human plasmin in cultured endothelial cells

Wen Chang Chang, Guey Yueh Shi, Ming T. Lin, Chauyin J. Jen, Lih Yuh C Wing, Hua Lin Wu

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Treatment of cultured bovine carotid artery endothelial cells with 10-7M plasmin increased the cellular diacylglycerol which was determined by the formation of [3H]palmitate-labeled diacylglycerol and diacylglycerol mass. Upon the stimulation with plasmin, a gradual increase in diacylglycerol formation was observed within 20 min then slightly declined. The maximal effect during the 1-h time course study was 45 and 55% increases in [3H]palmitate-labeled diacylgycerol and diacylglycerol mass, respectively, at 20 min after plasmin treatment. Formation of phosphatidylethanol was also studied in [3H]palmitate-prelabeled cells in the presence of ethanol. Treatment with plasmin for 20 min induced a significant 45% increase in phosphatidylethanol formation. The present results indicate that the plasmin-induced diacylglycerol formation in endothelial cells was at least in part mediated through the phospholipase D activation.

Original languageEnglish
Pages (from-to)89-95
Number of pages7
JournalThrombosis Research
Volume76
Issue number1
DOIs
Publication statusPublished - Oct 1 1994
Externally publishedYes

Keywords

  • diacylglycerol
  • endothelial cells
  • plasmin

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Hematology

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