Diacylglycerol formation induced by human plasmin in cultured endothelial cells

Wen Chang Chang, Guey Yueh Shi, Ming T. Lin, Chauyin J. Jen, Lih Yuh C. Wing, Hua Lin Wu

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Treatment of cultured bovine carotid artery endothelial cells with 10-7M plasmin increased the cellular diacylglycerol which was determined by the formation of [3H]palmitate-labeled diacylglycerol and diacylglycerol mass. Upon the stimulation with plasmin, a gradual increase in diacylglycerol formation was observed within 20 min then slightly declined. The maximal effect during the 1-h time course study was 45 and 55% increases in [3H]palmitate-labeled diacylgycerol and diacylglycerol mass, respectively, at 20 min after plasmin treatment. Formation of phosphatidylethanol was also studied in [3H]palmitate-prelabeled cells in the presence of ethanol. Treatment with plasmin for 20 min induced a significant 45% increase in phosphatidylethanol formation. The present results indicate that the plasmin-induced diacylglycerol formation in endothelial cells was at least in part mediated through the phospholipase D activation.

Original languageEnglish
Pages (from-to)89-95
Number of pages7
JournalThrombosis Research
Volume76
Issue number1
DOIs
Publication statusPublished - Oct 1 1994
Externally publishedYes

Keywords

  • diacylglycerol
  • endothelial cells
  • plasmin

ASJC Scopus subject areas

  • Hematology

Fingerprint Dive into the research topics of 'Diacylglycerol formation induced by human plasmin in cultured endothelial cells'. Together they form a unique fingerprint.

Cite this