Diabetic hyperglycemia aggravates seizures and status epilepticus-induced hippocampal damage

Chin Wei Huang, Juei Tang Cheng, Jing Jane Tsai, Sheng Nan Wu, Chao Ching Huang

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Epileptic seizures in diabetic hyperglycemia (DH) are not uncommon. This study aimed to determine the acute behavioral, pathological, and electrophysiological effects of status epilepticus (SE) on diabetic animals. Adult male Sprague-Dawley rats were first divided into groups with and without streptozotocin (STZ)-induced diabetes, and then into treatment groups given a normal saline (NS) (STZ-only and NS-only) or a lithium-pilocarpine injection to induce status epilepticus (STZ + SE and NS + SE). Seizure susceptibility, severity, and mortality were evaluated. Serial Morris water maze test and hippocampal histopathology results were examined before and 24 h after SE. Tetanic stimulation-induced long-term potentiation (LTP) in a hippocampal slice was recorded in a multi-electrode dish system. We also used a simulation model to evaluate intracellular adenosine triphosphate (ATP) and neuroexcitability. The STZ + SE group had a significantly higher percentage of severe seizures and SE-related death and worse learning and memory performances than the other three groups 24 h after SE. The STZ + SE group, and then the NS + SE group, showed the most severe neuronal loss and mossy fiber sprouting in the hippocampal CA3 area. In addition, LTP was markedly attenuated in the STZ + SE group, and then the NS + SE group. In the simulation, increased intracellular ATP concentration promoted action potential firing. This finding that rats with DH had more brain damage after SE than rats without diabetes suggests the importance of intensively treating hyperglycemia and seizures in diabetic patients with epilepsy.

Original languageEnglish
Pages (from-to)71-81
Number of pages11
JournalNeurotoxicity Research
Volume15
Issue number1
DOIs
Publication statusPublished - 2009
Externally publishedYes

Fingerprint

Status Epilepticus
Streptozocin
Hyperglycemia
Seizures
Rats
Medical problems
Adenosine Triphosphate
Pilocarpine
Long-Term Potentiation
Lithium
Brain
Animals
Epilepsy
Data storage equipment
Electrodes
Water
Fibers
Experimental Diabetes Mellitus
Action Potentials
Sprague Dawley Rats

Keywords

  • Diabetes
  • Seizures
  • Status epilepticus

ASJC Scopus subject areas

  • Neuroscience(all)
  • Toxicology

Cite this

Diabetic hyperglycemia aggravates seizures and status epilepticus-induced hippocampal damage. / Huang, Chin Wei; Cheng, Juei Tang; Tsai, Jing Jane; Wu, Sheng Nan; Huang, Chao Ching.

In: Neurotoxicity Research, Vol. 15, No. 1, 2009, p. 71-81.

Research output: Contribution to journalArticle

Huang, Chin Wei ; Cheng, Juei Tang ; Tsai, Jing Jane ; Wu, Sheng Nan ; Huang, Chao Ching. / Diabetic hyperglycemia aggravates seizures and status epilepticus-induced hippocampal damage. In: Neurotoxicity Research. 2009 ; Vol. 15, No. 1. pp. 71-81.
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