49 Citations (Scopus)

Abstract

Aim of the study: Upregulation of pulmonary inflammatory molecules is crucial in mediating the development of acute lung injury induced by haemorrhagic shock. Dexmedetomidine and ketamine possess potent anti-inflammatory capacity. We sought to elucidate whether dexmedetomidine, ketamine, or dexmedetomidine-ketamine combination could mitigate acute lung injury in haemorrhagic shock rats. Methods: Fifty adult male Sprague-Dawley rats were randomized to the sham-instrumented, haemorrhagic shock (HS), HS plus dexmedetomidine (HS-D), HS plus ketamine (HS-K), or HS plus dexmedetomidine-ketamine (HS-D + K) group (n = 10 in each group). Haemorrhagic shock was induced by blood withdrawing and the mean blood pressure was maintained at 40-45 mmHg for 120 min. Resuscitation was then performed by infusion of shed blood/saline mixtures. After monitoring for another 8 h, rats were sacrificed. Results: Histology findings and lung injury score analysis revealed moderate lung injury in rats of the HS, HS-D, and HS-K groups, whereas those of the HS-D + K group revealed mild lung injury. The effects of haemorrhagic shock on increasing cell number and protein concentration in bronchoalveolar lavage fluid as well as water content, leukocyte infiltration, and myeloperoxidase activity of lung tissues were significantly attenuated by dexmedetomidine-ketamine combination but not by dexmedetomidine or ketamine alone. Dexmedetomidine-ketamine combination, but not dexmedetomidine or ketamine alone, also significantly inhibited haemorrhagic shock-induced upregulation of pulmonary inflammatory molecules, including nitric oxide, prostaglandin E2, chemokine (e.g., macrophage inflammatory protein-2), and cytokines [e.g., interleukin (IL)-1β, and IL-6]. Conclusions: Dexmedetomidine-ketamine combination mitigates acute lung injury in haemorrhagic shock rats.

Original languageEnglish
Pages (from-to)1204-1210
Number of pages7
JournalResuscitation
Volume80
Issue number10
DOIs
Publication statusPublished - Oct 2009

Fingerprint

Dexmedetomidine
Hemorrhagic Shock
Acute Lung Injury
Ketamine
Lung Injury
Lung
Up-Regulation
Chemokine CXCL2
Bronchoalveolar Lavage Fluid

Keywords

  • Cyclooxygenase-2
  • Cytokine
  • Inducible nitric oxide synthase
  • Inflammation
  • Resuscitation

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Emergency Medicine
  • Emergency

Cite this

Dexmedetomidine-ketamine combination mitigates acute lung injury in haemorrhagic shock rats. / Yang, Chen Hsien; Tsai, Pei-Shan; Wang, Tao Yeuan; Huang, Chun J.

In: Resuscitation, Vol. 80, No. 10, 10.2009, p. 1204-1210.

Research output: Contribution to journalArticle

@article{b703a22a5af148a38e970d0bc59c74fa,
title = "Dexmedetomidine-ketamine combination mitigates acute lung injury in haemorrhagic shock rats",
abstract = "Aim of the study: Upregulation of pulmonary inflammatory molecules is crucial in mediating the development of acute lung injury induced by haemorrhagic shock. Dexmedetomidine and ketamine possess potent anti-inflammatory capacity. We sought to elucidate whether dexmedetomidine, ketamine, or dexmedetomidine-ketamine combination could mitigate acute lung injury in haemorrhagic shock rats. Methods: Fifty adult male Sprague-Dawley rats were randomized to the sham-instrumented, haemorrhagic shock (HS), HS plus dexmedetomidine (HS-D), HS plus ketamine (HS-K), or HS plus dexmedetomidine-ketamine (HS-D + K) group (n = 10 in each group). Haemorrhagic shock was induced by blood withdrawing and the mean blood pressure was maintained at 40-45 mmHg for 120 min. Resuscitation was then performed by infusion of shed blood/saline mixtures. After monitoring for another 8 h, rats were sacrificed. Results: Histology findings and lung injury score analysis revealed moderate lung injury in rats of the HS, HS-D, and HS-K groups, whereas those of the HS-D + K group revealed mild lung injury. The effects of haemorrhagic shock on increasing cell number and protein concentration in bronchoalveolar lavage fluid as well as water content, leukocyte infiltration, and myeloperoxidase activity of lung tissues were significantly attenuated by dexmedetomidine-ketamine combination but not by dexmedetomidine or ketamine alone. Dexmedetomidine-ketamine combination, but not dexmedetomidine or ketamine alone, also significantly inhibited haemorrhagic shock-induced upregulation of pulmonary inflammatory molecules, including nitric oxide, prostaglandin E2, chemokine (e.g., macrophage inflammatory protein-2), and cytokines [e.g., interleukin (IL)-1β, and IL-6]. Conclusions: Dexmedetomidine-ketamine combination mitigates acute lung injury in haemorrhagic shock rats.",
keywords = "Cyclooxygenase-2, Cytokine, Inducible nitric oxide synthase, Inflammation, Resuscitation",
author = "Yang, {Chen Hsien} and Pei-Shan Tsai and Wang, {Tao Yeuan} and Huang, {Chun J.}",
year = "2009",
month = "10",
doi = "10.1016/j.resuscitation.2009.06.017",
language = "English",
volume = "80",
pages = "1204--1210",
journal = "Resuscitation",
issn = "0300-9572",
publisher = "Elsevier Ireland Ltd",
number = "10",

}

TY - JOUR

T1 - Dexmedetomidine-ketamine combination mitigates acute lung injury in haemorrhagic shock rats

AU - Yang, Chen Hsien

AU - Tsai, Pei-Shan

AU - Wang, Tao Yeuan

AU - Huang, Chun J.

PY - 2009/10

Y1 - 2009/10

N2 - Aim of the study: Upregulation of pulmonary inflammatory molecules is crucial in mediating the development of acute lung injury induced by haemorrhagic shock. Dexmedetomidine and ketamine possess potent anti-inflammatory capacity. We sought to elucidate whether dexmedetomidine, ketamine, or dexmedetomidine-ketamine combination could mitigate acute lung injury in haemorrhagic shock rats. Methods: Fifty adult male Sprague-Dawley rats were randomized to the sham-instrumented, haemorrhagic shock (HS), HS plus dexmedetomidine (HS-D), HS plus ketamine (HS-K), or HS plus dexmedetomidine-ketamine (HS-D + K) group (n = 10 in each group). Haemorrhagic shock was induced by blood withdrawing and the mean blood pressure was maintained at 40-45 mmHg for 120 min. Resuscitation was then performed by infusion of shed blood/saline mixtures. After monitoring for another 8 h, rats were sacrificed. Results: Histology findings and lung injury score analysis revealed moderate lung injury in rats of the HS, HS-D, and HS-K groups, whereas those of the HS-D + K group revealed mild lung injury. The effects of haemorrhagic shock on increasing cell number and protein concentration in bronchoalveolar lavage fluid as well as water content, leukocyte infiltration, and myeloperoxidase activity of lung tissues were significantly attenuated by dexmedetomidine-ketamine combination but not by dexmedetomidine or ketamine alone. Dexmedetomidine-ketamine combination, but not dexmedetomidine or ketamine alone, also significantly inhibited haemorrhagic shock-induced upregulation of pulmonary inflammatory molecules, including nitric oxide, prostaglandin E2, chemokine (e.g., macrophage inflammatory protein-2), and cytokines [e.g., interleukin (IL)-1β, and IL-6]. Conclusions: Dexmedetomidine-ketamine combination mitigates acute lung injury in haemorrhagic shock rats.

AB - Aim of the study: Upregulation of pulmonary inflammatory molecules is crucial in mediating the development of acute lung injury induced by haemorrhagic shock. Dexmedetomidine and ketamine possess potent anti-inflammatory capacity. We sought to elucidate whether dexmedetomidine, ketamine, or dexmedetomidine-ketamine combination could mitigate acute lung injury in haemorrhagic shock rats. Methods: Fifty adult male Sprague-Dawley rats were randomized to the sham-instrumented, haemorrhagic shock (HS), HS plus dexmedetomidine (HS-D), HS plus ketamine (HS-K), or HS plus dexmedetomidine-ketamine (HS-D + K) group (n = 10 in each group). Haemorrhagic shock was induced by blood withdrawing and the mean blood pressure was maintained at 40-45 mmHg for 120 min. Resuscitation was then performed by infusion of shed blood/saline mixtures. After monitoring for another 8 h, rats were sacrificed. Results: Histology findings and lung injury score analysis revealed moderate lung injury in rats of the HS, HS-D, and HS-K groups, whereas those of the HS-D + K group revealed mild lung injury. The effects of haemorrhagic shock on increasing cell number and protein concentration in bronchoalveolar lavage fluid as well as water content, leukocyte infiltration, and myeloperoxidase activity of lung tissues were significantly attenuated by dexmedetomidine-ketamine combination but not by dexmedetomidine or ketamine alone. Dexmedetomidine-ketamine combination, but not dexmedetomidine or ketamine alone, also significantly inhibited haemorrhagic shock-induced upregulation of pulmonary inflammatory molecules, including nitric oxide, prostaglandin E2, chemokine (e.g., macrophage inflammatory protein-2), and cytokines [e.g., interleukin (IL)-1β, and IL-6]. Conclusions: Dexmedetomidine-ketamine combination mitigates acute lung injury in haemorrhagic shock rats.

KW - Cyclooxygenase-2

KW - Cytokine

KW - Inducible nitric oxide synthase

KW - Inflammation

KW - Resuscitation

UR - http://www.scopus.com/inward/record.url?scp=70149090356&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70149090356&partnerID=8YFLogxK

U2 - 10.1016/j.resuscitation.2009.06.017

DO - 10.1016/j.resuscitation.2009.06.017

M3 - Article

C2 - 19608326

AN - SCOPUS:70149090356

VL - 80

SP - 1204

EP - 1210

JO - Resuscitation

JF - Resuscitation

SN - 0300-9572

IS - 10

ER -