Development of N,O-(carboxymethyl)chitosan/collagen matrixes as a wound dressing

Ray Neng Chen, Gen Ming Wang, Chien Ho Chen, Hsiu O. Ho, Ming Thau Sheu

Research output: Contribution to journalArticle

140 Citations (Scopus)

Abstract

In an attempt to accelerate wound healing by stimulating the recruitment of fibroblasts and improve the mechanical properties of collagen matrixes, N,O-(carboxymethyl)chitosan (NOCC) was incorporated into the backbone of a collagen (COL) matrix without or with chondroitin sulfate (CS) or an acellular dermal matrix (ADM). The result of a cell migration study demonstrated that the migration of fibroblasts was significantly enhanced by NOCC in a concentration-dependent manner. In the analysis with a dynamic mechanical analyzer, NOCC/CS/COL matrixes presented higher tensile strengths than did NOCC/ADM/COL matrixes. Skin fibroblasts cultured on the matrixes containing NOCC showed increased proliferation and secretion of three kinds of cytokines compared with the control. Results of the in vivo wound healing study showed that matrixes incorporating NOCC showed markedly enhanced wound healing compared with the control. Therefore, the above results clearly suggest that NOCC/COL matrixes containing CS or ADM can be potential wound dressings for clinical applications.

Original languageEnglish
Pages (from-to)1058-1064
Number of pages7
JournalBiomacromolecules
Volume7
Issue number4
DOIs
Publication statusPublished - Apr 2006

Fingerprint

Bandages
Chitosan
Collagen
Acellular Dermis
Wounds and Injuries
Chondroitin Sulfates
Fibroblasts
Wound Healing
Tensile Strength
O,N-carboxymethylchitosan
Cell Movement
Skin
Tensile strength
Cytokines
Mechanical properties
Sulfates

ASJC Scopus subject areas

  • Organic Chemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Polymers and Plastics
  • Materials Chemistry

Cite this

Development of N,O-(carboxymethyl)chitosan/collagen matrixes as a wound dressing. / Chen, Ray Neng; Wang, Gen Ming; Chen, Chien Ho; Ho, Hsiu O.; Sheu, Ming Thau.

In: Biomacromolecules, Vol. 7, No. 4, 04.2006, p. 1058-1064.

Research output: Contribution to journalArticle

Chen, Ray Neng ; Wang, Gen Ming ; Chen, Chien Ho ; Ho, Hsiu O. ; Sheu, Ming Thau. / Development of N,O-(carboxymethyl)chitosan/collagen matrixes as a wound dressing. In: Biomacromolecules. 2006 ; Vol. 7, No. 4. pp. 1058-1064.
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