Development of Irinotecan Liposome Armed with Dual-Target Anti-Epidermal Growth Factor Receptor and Anti-Fibroblast Activation Protein-Specific Antibody for Pancreatic Cancer Treatment

Hung Jun Lin, Tien Li Liang, Yao Yuan Chang, Der Zen Liu, Jia Yu Fan, Steve R. Roffler, Shyr Yi Lin

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Pancreatic cancer is one of the most common causes of death in Taiwan. Previous studies have shown that more than 90% of pancreatic cancer cells presented epidermal growth factor receptor (EGFR) cell marker, and this marker is thought to be important as it is related to activation of cancer cell proliferation, angiogenesis, and cancer progression. Moreover, tumor-associated fibroblasts were involved in tumor proliferation and progression. In this study, we fabricated an anti-EGFR and anti-fibroblast activation protein bispecific antibody-targeted liposomal irinotecan (BS−LipoIRI), which could specifically bind to pancreatic cancer cells and tumor-associated fibroblasts. The drug encapsulation efficiency of BS−LipoIRI was 80.95%, and the drug loading was 8.41%. We proved that both pancreatic cancer cells and fibroblasts could be targeted by BS−LipoIRI, which showed better cellular uptake efficacy compared to LipoIRI. Furthermore, an in vivo mouse tumor test indicated that BS−LipoIRI could inhibit pancreatic cancer growth up to 46.2% compared to phosphate-buffered saline control, suggesting that BS−LipoIRI could be useful in clinical cancer treatment.

Original languageEnglish
Article number1202
JournalPharmaceutics
Volume14
Issue number6
DOIs
Publication statusPublished - Jun 2022

Keywords

  • bispecific targeting antibody
  • liposomal drug
  • pancreatic cancer
  • targeted liposome

ASJC Scopus subject areas

  • Pharmaceutical Science

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