Development of immunoassays for serum tartrate-resistant acid phosphatase isoform 5a

Tsu Yi Chao, Su Huei Lee, Mary M. Chen, David H. Neustadt, Uzma A. Chaudhry, Lung T. Yam, Anthony J. Janckila

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Background: Serum tartrate-resistant acid phosphatase (TRACP) consists of 2 structurally related isoforms, TRACP 5a and 5b. TRACP 5b is from bone-resorbing osteoclasts. TRACP 5a may be a macrophage product of inflammation. We used a novel antibody to TRACP 5a to standardize immunoassays for serum TRACP 5a activity and protein. Methods: Biotinylated anti-TRACP antibodies were used to immobilize serum TRACP isoforms. TRACP activity was measured using 4-nitrophenyl phosphate as substrate. TRACP 5a protein was measured with an independent peroxidase-conjugated anti-TRACP antibody. Immunoassays were standardized for linearity of serum dose response, sensitivity and precision. Reference ranges for TRACP 5a were established from serum of 50 healthy males and 50 healthy age-matched females. Serum TRACP 5a activity and protein were determined in 29 cases of rheumatoid arthritis. Results: Serum matrix interference in both TRACP 5a assays required dilution to 10% serum to approach linearity. Intra-assay and inter-assay CV% were

Original languageEnglish
Pages (from-to)132-140
Number of pages9
JournalClinica Chimica Acta
Volume359
Issue number1-2
DOIs
Publication statusPublished - Sep 2005
Externally publishedYes

Fingerprint

Acid Phosphatase
Immunoassay
Protein Isoforms
Serum
Assays
Tartrate-Resistant Acid Phosphatase
tartaric acid
Antibodies
Proteins
Macrophages
Osteoclasts
Peroxidase
Dilution
Rheumatoid Arthritis
Bone
Reference Values

Keywords

  • Acid phosphatase
  • Bone resorption
  • Immunoassay
  • Inflammation

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

Cite this

Chao, T. Y., Lee, S. H., Chen, M. M., Neustadt, D. H., Chaudhry, U. A., Yam, L. T., & Janckila, A. J. (2005). Development of immunoassays for serum tartrate-resistant acid phosphatase isoform 5a. Clinica Chimica Acta, 359(1-2), 132-140. https://doi.org/10.1016/j.cccn.2005.03.039

Development of immunoassays for serum tartrate-resistant acid phosphatase isoform 5a. / Chao, Tsu Yi; Lee, Su Huei; Chen, Mary M.; Neustadt, David H.; Chaudhry, Uzma A.; Yam, Lung T.; Janckila, Anthony J.

In: Clinica Chimica Acta, Vol. 359, No. 1-2, 09.2005, p. 132-140.

Research output: Contribution to journalArticle

Chao, TY, Lee, SH, Chen, MM, Neustadt, DH, Chaudhry, UA, Yam, LT & Janckila, AJ 2005, 'Development of immunoassays for serum tartrate-resistant acid phosphatase isoform 5a', Clinica Chimica Acta, vol. 359, no. 1-2, pp. 132-140. https://doi.org/10.1016/j.cccn.2005.03.039
Chao, Tsu Yi ; Lee, Su Huei ; Chen, Mary M. ; Neustadt, David H. ; Chaudhry, Uzma A. ; Yam, Lung T. ; Janckila, Anthony J. / Development of immunoassays for serum tartrate-resistant acid phosphatase isoform 5a. In: Clinica Chimica Acta. 2005 ; Vol. 359, No. 1-2. pp. 132-140.
@article{2f109e2d71d7453aada7853156f49304,
title = "Development of immunoassays for serum tartrate-resistant acid phosphatase isoform 5a",
abstract = "Background: Serum tartrate-resistant acid phosphatase (TRACP) consists of 2 structurally related isoforms, TRACP 5a and 5b. TRACP 5b is from bone-resorbing osteoclasts. TRACP 5a may be a macrophage product of inflammation. We used a novel antibody to TRACP 5a to standardize immunoassays for serum TRACP 5a activity and protein. Methods: Biotinylated anti-TRACP antibodies were used to immobilize serum TRACP isoforms. TRACP activity was measured using 4-nitrophenyl phosphate as substrate. TRACP 5a protein was measured with an independent peroxidase-conjugated anti-TRACP antibody. Immunoassays were standardized for linearity of serum dose response, sensitivity and precision. Reference ranges for TRACP 5a were established from serum of 50 healthy males and 50 healthy age-matched females. Serum TRACP 5a activity and protein were determined in 29 cases of rheumatoid arthritis. Results: Serum matrix interference in both TRACP 5a assays required dilution to 10{\%} serum to approach linearity. Intra-assay and inter-assay CV{\%} were",
keywords = "Acid phosphatase, Bone resorption, Immunoassay, Inflammation",
author = "Chao, {Tsu Yi} and Lee, {Su Huei} and Chen, {Mary M.} and Neustadt, {David H.} and Chaudhry, {Uzma A.} and Yam, {Lung T.} and Janckila, {Anthony J.}",
year = "2005",
month = "9",
doi = "10.1016/j.cccn.2005.03.039",
language = "English",
volume = "359",
pages = "132--140",
journal = "Clinica Chimica Acta",
issn = "0009-8981",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Development of immunoassays for serum tartrate-resistant acid phosphatase isoform 5a

AU - Chao, Tsu Yi

AU - Lee, Su Huei

AU - Chen, Mary M.

AU - Neustadt, David H.

AU - Chaudhry, Uzma A.

AU - Yam, Lung T.

AU - Janckila, Anthony J.

PY - 2005/9

Y1 - 2005/9

N2 - Background: Serum tartrate-resistant acid phosphatase (TRACP) consists of 2 structurally related isoforms, TRACP 5a and 5b. TRACP 5b is from bone-resorbing osteoclasts. TRACP 5a may be a macrophage product of inflammation. We used a novel antibody to TRACP 5a to standardize immunoassays for serum TRACP 5a activity and protein. Methods: Biotinylated anti-TRACP antibodies were used to immobilize serum TRACP isoforms. TRACP activity was measured using 4-nitrophenyl phosphate as substrate. TRACP 5a protein was measured with an independent peroxidase-conjugated anti-TRACP antibody. Immunoassays were standardized for linearity of serum dose response, sensitivity and precision. Reference ranges for TRACP 5a were established from serum of 50 healthy males and 50 healthy age-matched females. Serum TRACP 5a activity and protein were determined in 29 cases of rheumatoid arthritis. Results: Serum matrix interference in both TRACP 5a assays required dilution to 10% serum to approach linearity. Intra-assay and inter-assay CV% were

AB - Background: Serum tartrate-resistant acid phosphatase (TRACP) consists of 2 structurally related isoforms, TRACP 5a and 5b. TRACP 5b is from bone-resorbing osteoclasts. TRACP 5a may be a macrophage product of inflammation. We used a novel antibody to TRACP 5a to standardize immunoassays for serum TRACP 5a activity and protein. Methods: Biotinylated anti-TRACP antibodies were used to immobilize serum TRACP isoforms. TRACP activity was measured using 4-nitrophenyl phosphate as substrate. TRACP 5a protein was measured with an independent peroxidase-conjugated anti-TRACP antibody. Immunoassays were standardized for linearity of serum dose response, sensitivity and precision. Reference ranges for TRACP 5a were established from serum of 50 healthy males and 50 healthy age-matched females. Serum TRACP 5a activity and protein were determined in 29 cases of rheumatoid arthritis. Results: Serum matrix interference in both TRACP 5a assays required dilution to 10% serum to approach linearity. Intra-assay and inter-assay CV% were

KW - Acid phosphatase

KW - Bone resorption

KW - Immunoassay

KW - Inflammation

UR - http://www.scopus.com/inward/record.url?scp=23644459497&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=23644459497&partnerID=8YFLogxK

U2 - 10.1016/j.cccn.2005.03.039

DO - 10.1016/j.cccn.2005.03.039

M3 - Article

C2 - 15993395

AN - SCOPUS:23644459497

VL - 359

SP - 132

EP - 140

JO - Clinica Chimica Acta

JF - Clinica Chimica Acta

SN - 0009-8981

IS - 1-2

ER -