Development of immunoassays for serum tartrate-resistant acid phosphatase isoform 5a

Tsu Yi Chao, Su Huei Lee, Mary M. Chen, David H. Neustadt, Uzma A. Chaudhry, Lung T. Yam, Anthony J. Janckila

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)


Background: Serum tartrate-resistant acid phosphatase (TRACP) consists of 2 structurally related isoforms, TRACP 5a and 5b. TRACP 5b is from bone-resorbing osteoclasts. TRACP 5a may be a macrophage product of inflammation. We used a novel antibody to TRACP 5a to standardize immunoassays for serum TRACP 5a activity and protein. Methods: Biotinylated anti-TRACP antibodies were used to immobilize serum TRACP isoforms. TRACP activity was measured using 4-nitrophenyl phosphate as substrate. TRACP 5a protein was measured with an independent peroxidase-conjugated anti-TRACP antibody. Immunoassays were standardized for linearity of serum dose response, sensitivity and precision. Reference ranges for TRACP 5a were established from serum of 50 healthy males and 50 healthy age-matched females. Serum TRACP 5a activity and protein were determined in 29 cases of rheumatoid arthritis. Results: Serum matrix interference in both TRACP 5a assays required dilution to 10% serum to approach linearity. Intra-assay and inter-assay CV% were

Original languageEnglish
Pages (from-to)132-140
Number of pages9
JournalClinica Chimica Acta
Issue number1-2
Publication statusPublished - Sep 2005
Externally publishedYes


  • Acid phosphatase
  • Bone resorption
  • Immunoassay
  • Inflammation

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

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