應用於骨缺損之快速塑形支架研發

Translated title of the contribution: Development of Fast Conformable Scaffold Applied in Bone Defects

Research output: Contribution to journalArticle

Abstract

Orthopaedic, craniomaxillofacial surgery and accidental injury frequently results in large bony defects require the bone grafts to enhance bone healing. Synthetic bone graft substitutes such as hydroxyapatite, tricalcium phosphate and calcium sulfate have shortcomings in the sense that they have prolonged working and setting time, as well as poor compressive strength. Therefore, the aim of this study was to develop a new, convenient and easily moldable bone filling material based on the poly-lactic acid (PLA)/polycaprolactone (PCL) matrix with a pore-former filler (calcium sulfate). The self-formation of interconnected pores within this thermo-moldable scaffold can be observed only after the implantation due to the fast absorption characteristic of calcium sulfate within the body. The optimum ratio of the composition is 18% PLA: 42% PCL: 40% CaSO4. The compressive strength, three point bending strength and elastic modulus were 38.2±1.2MPa, 67.5±7.2MPa, 7.0±0.7GPa, respectively while the shaping temperature was 50±2℃, the setting and hardening time did not exceed 6 minutes. Cytotoxicity revealed bullet-shaped and intact cells with no mutation or apoptosis present. In addition, good cell attachment was observed. MTT test indicated that cell viability observed in the experimental group is higher than 100%. After immersion in phosphate-buffered solution (PBS) for 8~12 weeks, 50-200μm sized pores were observed under scanning electron microscope (SEM). It will be the good choice for bone grafting.
Original languageTraditional Chinese
Pages (from-to)201-216
Number of pages16
Journal中華牙醫學雜誌(中文版)
Volume1
Issue number3
Publication statusPublished - 2005

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Calcium Sulfate
Compressive Strength
Bone and Bones
Transplants
Bone Substitutes
Bone Transplantation
Elastic Modulus
Immersion
Durapatite
Orthopedics
Cell Survival
Phosphates
Electrons
Apoptosis
Mutation
Temperature
Wounds and Injuries
poly(lactic acid)
polycaprolactone

Keywords

  • polylactic acid
  • polycaprolactone
  • calcium sulfate
  • bone graft
  • scaffold

Cite this

應用於骨缺損之快速塑形支架研發. / 楊正昌(Jen-Chang Yang); 李憲坤(Hsien-Kun Lee); 陳建中(Chien-Chung Chen); 鄧乃嘉(Nai-Chia Teng); 陳長志(Chang-Chih Chen); 李勝揚(Sheng-Yang Lee).

In: 中華牙醫學雜誌(中文版), Vol. 1, No. 3, 2005, p. 201-216.

Research output: Contribution to journalArticle

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abstract = "口腔及顱顏外科手術或意外傷害時,倘若造成大範圍之骨缺損,常因癒合不易而需要以骨移植術加速骨的癒合,目前雖有氫氧磷灰石,磷酸鈣與硫酸鈣合成骨替代物等選擇,但這些產品操作及固化時間長與抗壓強度較低,因而造成手術時間變長,因此本研究目的在研發一種新型且操作便利,可快速塑形,符合骨組織工程原理,應用於骨缺損的骨填補複合材。材料以聚乳酸與聚己內酯為基體,選擇材料分解速率大於骨癒合增生速率之硫酸鈣為填充物,利用其過水快速溶解吸收的特性,自然形成具連續性孔洞的支架。找出最佳化條件並對此支架進行物理與機械性質測試,軟化塑形溫度,固化時間,細胞毒性測試及觀察孔洞的形成。結果顯示上述三種材料依序的配比為18:42:40(wt{\%})具有最佳機械性質,其抗壓強度、三點彎曲強度與彈性係數分別為38.2±1.2MPa、67.5±7.2MPa及7.0±0.7GPa,塑形溫度50±2℃,固化時間不超過6分鐘,細胞毒性及細胞貼附性測試顯示細胞成梭型且外型完整,並無突起變細或細胞凋亡情形,且細胞對骨填補材的貼附性良好,MTT test亦顯示細胞存活率高,浸泡於磷酸緩衝溶液經8~12週以掃描式電子顯微鏡觀察可見50~200μm的孔洞結構,相較於傳統材料具有較佳強度,塑形溫度低,固化時間短,具骨填補材料應用之潛力。",
keywords = "聚乳酸, 聚己內酯, 硫酸鈣, 骨移植物, 支架, polylactic acid, polycaprolactone, calcium sulfate, bone graft, scaffold",
author = "楊, {正昌(Jen-Chang Yang)} and 李, {憲坤(Hsien-Kun Lee)} and 陳, {建中(Chien-Chung Chen)} and 鄧, {乃嘉(Nai-Chia Teng)} and 陳, {長志(Chang-Chih Chen)} and 李, {勝揚(Sheng-Yang Lee)}",
year = "2005",
language = "繁體中文",
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publisher = "中華牙醫學會",
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TY - JOUR

T1 - 應用於骨缺損之快速塑形支架研發

AU - 楊, 正昌(Jen-Chang Yang)

AU - 李, 憲坤(Hsien-Kun Lee)

AU - 陳, 建中(Chien-Chung Chen)

AU - 鄧, 乃嘉(Nai-Chia Teng)

AU - 陳, 長志(Chang-Chih Chen)

AU - 李, 勝揚(Sheng-Yang Lee)

PY - 2005

Y1 - 2005

N2 - 口腔及顱顏外科手術或意外傷害時,倘若造成大範圍之骨缺損,常因癒合不易而需要以骨移植術加速骨的癒合,目前雖有氫氧磷灰石,磷酸鈣與硫酸鈣合成骨替代物等選擇,但這些產品操作及固化時間長與抗壓強度較低,因而造成手術時間變長,因此本研究目的在研發一種新型且操作便利,可快速塑形,符合骨組織工程原理,應用於骨缺損的骨填補複合材。材料以聚乳酸與聚己內酯為基體,選擇材料分解速率大於骨癒合增生速率之硫酸鈣為填充物,利用其過水快速溶解吸收的特性,自然形成具連續性孔洞的支架。找出最佳化條件並對此支架進行物理與機械性質測試,軟化塑形溫度,固化時間,細胞毒性測試及觀察孔洞的形成。結果顯示上述三種材料依序的配比為18:42:40(wt%)具有最佳機械性質,其抗壓強度、三點彎曲強度與彈性係數分別為38.2±1.2MPa、67.5±7.2MPa及7.0±0.7GPa,塑形溫度50±2℃,固化時間不超過6分鐘,細胞毒性及細胞貼附性測試顯示細胞成梭型且外型完整,並無突起變細或細胞凋亡情形,且細胞對骨填補材的貼附性良好,MTT test亦顯示細胞存活率高,浸泡於磷酸緩衝溶液經8~12週以掃描式電子顯微鏡觀察可見50~200μm的孔洞結構,相較於傳統材料具有較佳強度,塑形溫度低,固化時間短,具骨填補材料應用之潛力。

AB - 口腔及顱顏外科手術或意外傷害時,倘若造成大範圍之骨缺損,常因癒合不易而需要以骨移植術加速骨的癒合,目前雖有氫氧磷灰石,磷酸鈣與硫酸鈣合成骨替代物等選擇,但這些產品操作及固化時間長與抗壓強度較低,因而造成手術時間變長,因此本研究目的在研發一種新型且操作便利,可快速塑形,符合骨組織工程原理,應用於骨缺損的骨填補複合材。材料以聚乳酸與聚己內酯為基體,選擇材料分解速率大於骨癒合增生速率之硫酸鈣為填充物,利用其過水快速溶解吸收的特性,自然形成具連續性孔洞的支架。找出最佳化條件並對此支架進行物理與機械性質測試,軟化塑形溫度,固化時間,細胞毒性測試及觀察孔洞的形成。結果顯示上述三種材料依序的配比為18:42:40(wt%)具有最佳機械性質,其抗壓強度、三點彎曲強度與彈性係數分別為38.2±1.2MPa、67.5±7.2MPa及7.0±0.7GPa,塑形溫度50±2℃,固化時間不超過6分鐘,細胞毒性及細胞貼附性測試顯示細胞成梭型且外型完整,並無突起變細或細胞凋亡情形,且細胞對骨填補材的貼附性良好,MTT test亦顯示細胞存活率高,浸泡於磷酸緩衝溶液經8~12週以掃描式電子顯微鏡觀察可見50~200μm的孔洞結構,相較於傳統材料具有較佳強度,塑形溫度低,固化時間短,具骨填補材料應用之潛力。

KW - 聚乳酸

KW - 聚己內酯

KW - 硫酸鈣

KW - 骨移植物

KW - 支架

KW - polylactic acid

KW - polycaprolactone

KW - calcium sulfate

KW - bone graft

KW - scaffold

M3 - 文章

VL - 1

SP - 201

EP - 216

JO - 中華牙醫學雜誌(中文版)

JF - 中華牙醫學雜誌(中文版)

SN - 1010-3287

IS - 3

ER -