Development of bovine serum albumin-modified hybrid nanoclusters for magnetofluorescence imaging and drug delivery

Mochamad Zakki Fahmi, Keng Liang Ou, Jem Kun Chen, Ming Hua Ho, Shin Hwa Tzing, Jia Yaw Chang

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

In this study, bovine serum albumin (BSA) was used for simultaneously clustering and phase-transferring both oil-soluble AgInS2-ZnS quantum dots (QDs) and MnFe2O4 magnetic nanoparticles (MNPs) under ultrasonication. The hybrid nanoclusters, BSA(QMs), thus produced were conjugated with folic acid (FA) and doxorubicin (DOX) to improve their target specificity and drug delivery to HeLa cancer cells. The resulting nanoclusters were characterized by employing different analytical techniques, and the results showed the nanocluster magnetofluorescence property derived from the clustering process. It was also found that the hybrid nanoclusters were biocompatible, non-toxic, and considerably stable over a wide range of pH values and at high ionic strengths. In addition, the in vitro confocal microscopy and MR relaxation studies revealed the yellow fluorescence and T2 contrast-enhancing property of FA-BSA(QMs), as well as their cellular pathway to enter HeLa cells via folate receptor-mediated endocytosis. Furthermore, the cell viability data and flow cytometry results demonstrated the selective uptake of DOX-FA-BSA(QMs) by the HeLa cells, which significantly enhanced cell cytotoxicity. These results suggest that the proposed nanoclusters can be used as an effective and efficient strategy for magnetofluorescent probing and cancer drug delivery. This journal is

Original languageEnglish
Pages (from-to)32762-32772
Number of pages11
JournalRSC Advances
Volume4
Issue number62
DOIs
Publication statusPublished - 2014

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Chemistry(all)

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