Development of bovine serum albumin-modified hybrid nanoclusters for magnetofluorescence imaging and drug delivery

Mochamad Zakki Fahmi, Keng Liang Ou, Jem Kun Chen, Ming Hua Ho, Shin Hwa Tzing, Jia Yaw Chang

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

In this study, bovine serum albumin (BSA) was used for simultaneously clustering and phase-transferring both oil-soluble AgInS2-ZnS quantum dots (QDs) and MnFe2O4 magnetic nanoparticles (MNPs) under ultrasonication. The hybrid nanoclusters, BSA(QMs), thus produced were conjugated with folic acid (FA) and doxorubicin (DOX) to improve their target specificity and drug delivery to HeLa cancer cells. The resulting nanoclusters were characterized by employing different analytical techniques, and the results showed the nanocluster magnetofluorescence property derived from the clustering process. It was also found that the hybrid nanoclusters were biocompatible, non-toxic, and considerably stable over a wide range of pH values and at high ionic strengths. In addition, the in vitro confocal microscopy and MR relaxation studies revealed the yellow fluorescence and T2 contrast-enhancing property of FA-BSA(QMs), as well as their cellular pathway to enter HeLa cells via folate receptor-mediated endocytosis. Furthermore, the cell viability data and flow cytometry results demonstrated the selective uptake of DOX-FA-BSA(QMs) by the HeLa cells, which significantly enhanced cell cytotoxicity. These results suggest that the proposed nanoclusters can be used as an effective and efficient strategy for magnetofluorescent probing and cancer drug delivery. This journal is

Original languageEnglish
Pages (from-to)32762-32772
Number of pages11
JournalRSC Advances
Volume4
Issue number62
DOIs
Publication statusPublished - 2014

Fingerprint

Nanoclusters
Bovine Serum Albumin
Drug delivery
Folic Acid
Imaging techniques
Doxorubicin
Acids
Cells
Flow cytometry
Confocal microscopy
Cytotoxicity
Ionic strength
Semiconductor quantum dots
Oils
Fluorescence
Nanoparticles

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Chemistry(all)

Cite this

Fahmi, M. Z., Ou, K. L., Chen, J. K., Ho, M. H., Tzing, S. H., & Chang, J. Y. (2014). Development of bovine serum albumin-modified hybrid nanoclusters for magnetofluorescence imaging and drug delivery. RSC Advances, 4(62), 32762-32772. https://doi.org/10.1039/c4ra05785f

Development of bovine serum albumin-modified hybrid nanoclusters for magnetofluorescence imaging and drug delivery. / Fahmi, Mochamad Zakki; Ou, Keng Liang; Chen, Jem Kun; Ho, Ming Hua; Tzing, Shin Hwa; Chang, Jia Yaw.

In: RSC Advances, Vol. 4, No. 62, 2014, p. 32762-32772.

Research output: Contribution to journalArticle

Fahmi, Mochamad Zakki ; Ou, Keng Liang ; Chen, Jem Kun ; Ho, Ming Hua ; Tzing, Shin Hwa ; Chang, Jia Yaw. / Development of bovine serum albumin-modified hybrid nanoclusters for magnetofluorescence imaging and drug delivery. In: RSC Advances. 2014 ; Vol. 4, No. 62. pp. 32762-32772.
@article{b3ce1ab9adc84ce58f9967bcb23e602f,
title = "Development of bovine serum albumin-modified hybrid nanoclusters for magnetofluorescence imaging and drug delivery",
abstract = "In this study, bovine serum albumin (BSA) was used for simultaneously clustering and phase-transferring both oil-soluble AgInS2-ZnS quantum dots (QDs) and MnFe2O4 magnetic nanoparticles (MNPs) under ultrasonication. The hybrid nanoclusters, BSA(QMs), thus produced were conjugated with folic acid (FA) and doxorubicin (DOX) to improve their target specificity and drug delivery to HeLa cancer cells. The resulting nanoclusters were characterized by employing different analytical techniques, and the results showed the nanocluster magnetofluorescence property derived from the clustering process. It was also found that the hybrid nanoclusters were biocompatible, non-toxic, and considerably stable over a wide range of pH values and at high ionic strengths. In addition, the in vitro confocal microscopy and MR relaxation studies revealed the yellow fluorescence and T2 contrast-enhancing property of FA-BSA(QMs), as well as their cellular pathway to enter HeLa cells via folate receptor-mediated endocytosis. Furthermore, the cell viability data and flow cytometry results demonstrated the selective uptake of DOX-FA-BSA(QMs) by the HeLa cells, which significantly enhanced cell cytotoxicity. These results suggest that the proposed nanoclusters can be used as an effective and efficient strategy for magnetofluorescent probing and cancer drug delivery. This journal is",
author = "Fahmi, {Mochamad Zakki} and Ou, {Keng Liang} and Chen, {Jem Kun} and Ho, {Ming Hua} and Tzing, {Shin Hwa} and Chang, {Jia Yaw}",
year = "2014",
doi = "10.1039/c4ra05785f",
language = "English",
volume = "4",
pages = "32762--32772",
journal = "RSC Advances",
issn = "2046-2069",
publisher = "The Royal Society of Chemistry",
number = "62",

}

TY - JOUR

T1 - Development of bovine serum albumin-modified hybrid nanoclusters for magnetofluorescence imaging and drug delivery

AU - Fahmi, Mochamad Zakki

AU - Ou, Keng Liang

AU - Chen, Jem Kun

AU - Ho, Ming Hua

AU - Tzing, Shin Hwa

AU - Chang, Jia Yaw

PY - 2014

Y1 - 2014

N2 - In this study, bovine serum albumin (BSA) was used for simultaneously clustering and phase-transferring both oil-soluble AgInS2-ZnS quantum dots (QDs) and MnFe2O4 magnetic nanoparticles (MNPs) under ultrasonication. The hybrid nanoclusters, BSA(QMs), thus produced were conjugated with folic acid (FA) and doxorubicin (DOX) to improve their target specificity and drug delivery to HeLa cancer cells. The resulting nanoclusters were characterized by employing different analytical techniques, and the results showed the nanocluster magnetofluorescence property derived from the clustering process. It was also found that the hybrid nanoclusters were biocompatible, non-toxic, and considerably stable over a wide range of pH values and at high ionic strengths. In addition, the in vitro confocal microscopy and MR relaxation studies revealed the yellow fluorescence and T2 contrast-enhancing property of FA-BSA(QMs), as well as their cellular pathway to enter HeLa cells via folate receptor-mediated endocytosis. Furthermore, the cell viability data and flow cytometry results demonstrated the selective uptake of DOX-FA-BSA(QMs) by the HeLa cells, which significantly enhanced cell cytotoxicity. These results suggest that the proposed nanoclusters can be used as an effective and efficient strategy for magnetofluorescent probing and cancer drug delivery. This journal is

AB - In this study, bovine serum albumin (BSA) was used for simultaneously clustering and phase-transferring both oil-soluble AgInS2-ZnS quantum dots (QDs) and MnFe2O4 magnetic nanoparticles (MNPs) under ultrasonication. The hybrid nanoclusters, BSA(QMs), thus produced were conjugated with folic acid (FA) and doxorubicin (DOX) to improve their target specificity and drug delivery to HeLa cancer cells. The resulting nanoclusters were characterized by employing different analytical techniques, and the results showed the nanocluster magnetofluorescence property derived from the clustering process. It was also found that the hybrid nanoclusters were biocompatible, non-toxic, and considerably stable over a wide range of pH values and at high ionic strengths. In addition, the in vitro confocal microscopy and MR relaxation studies revealed the yellow fluorescence and T2 contrast-enhancing property of FA-BSA(QMs), as well as their cellular pathway to enter HeLa cells via folate receptor-mediated endocytosis. Furthermore, the cell viability data and flow cytometry results demonstrated the selective uptake of DOX-FA-BSA(QMs) by the HeLa cells, which significantly enhanced cell cytotoxicity. These results suggest that the proposed nanoclusters can be used as an effective and efficient strategy for magnetofluorescent probing and cancer drug delivery. This journal is

UR - http://www.scopus.com/inward/record.url?scp=84905758111&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84905758111&partnerID=8YFLogxK

U2 - 10.1039/c4ra05785f

DO - 10.1039/c4ra05785f

M3 - Article

VL - 4

SP - 32762

EP - 32772

JO - RSC Advances

JF - RSC Advances

SN - 2046-2069

IS - 62

ER -