Development of an RPLC-based method for measurement of indoprofen stability and validation of the method

Yi B. Liou, Fu A. Chen, Jen A. Lee, Hsiu O. Ho, An-Bang Wu

Research output: Contribution to journalArticle

Abstract

A method for quantitative measurement of the photochemical decomposition of the anti-inflammatory agent, indoprofen (INP) is descriped. An RPLC-based assay that could determine the extent of degradation of INP in a rapid, sensitive, and accurate manner was developed. The method was validated under photoirradiation. Quantitation was monitored with an Inertsil ODS-3V column using a mobile phase of acetonitril and 1% HOAc solution in deionized H 2O. Statistics relevant to the system criteria, peak integrity and resolution among the parent drug and its degradation products were performed. From the intra- and inter-day tests, the coefficients of variation were found to range from 0.59% to 4.25% for the former and from 0.71% to 4.86% for the latter. The good selectivity and specificity of this RPLC-based procedure render it suitable for measurements of INP stability.

Original languageEnglish
Pages (from-to)1570-1574
Number of pages5
JournalChinese Journal of Chemistry
Volume24
Issue number11
DOIs
Publication statusPublished - Nov 2006

Keywords

  • Indoprofen
  • Photodegradation
  • Quantitation

ASJC Scopus subject areas

  • Chemistry(all)

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