Development of an information-rich LC-MS/MS database for the analysis of drugs in postmortem specimens

Hsiu Chuan Liu, Ray H. Liu, Hsiu O. Ho, Dong Liang Lin

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

With several instrument configurations available from various manufacturers, liquid chromatography-tandem mass spectrometry (LC-MS/MS) technology is currently intensively studied for comprehensive drug screen and confirmation. An LC-MS/MS database, including 780 drug and toxic compounds, has been constructed, featuring information-rich MS/MS spectra derived from a novel fragmentation approach incorporating voltage ramping and broadened mass window for activation. The resulting spectra are rich in high- and low-mass fragment ions, highly effective for matching and proven reproducible over a 6 month test period. Coupled to effective sample preparation protocols, the database-searching process greatly improved the identification of drugs in postmortem specimens by the LC-electrospray ionization (ESI)-MS/MS technology. This method has significantly improved the efficiency of our routine laboratory operation that was based on a two-step [fluorescence polarization immunoassay (FPIA) and gas chromatography/mass spectrometry (GC/MS)] approach in the past.

Original languageEnglish
Pages (from-to)9002-9011
Number of pages10
JournalAnalytical Chemistry
Volume81
Issue number21
DOIs
Publication statusPublished - Nov 1 2009

ASJC Scopus subject areas

  • Analytical Chemistry

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