Development of an enzyme-linked immunosorbent assay for human plasma inter-α-trypsin inhibitor (ITI) using specific antibodies against each of the H1 and H2 heavy chains

C. Mizon, M. Balduyck, D. Albani, C. Michalski, T. Burnouf, J. Mizon

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Inter-α-trypsin inhibitor (ITI) is a serine-proteinase inhibitor of human plasma enzymes. ITI is composed of three polypeptide chains covalently linked: bikunin, responsible for the antiprotease activity and two heavy chains H1 and H2. Human plasma also contains other components immunologically related to ITI such as pre-α-trypsin inhibitor (pal), inter-α-like inhibitor (I α LI) and free bikunin. The ELISA procedure we propose exclusively measures native ITI within the range 12.5-200 μg/l. The intra- and interassay coefficients of variation were less than 5.6% and 8.7%, respectively. When ITI was added to plasma samples, full recovery was obtained, EDTA-plasma from 30 healthy individuals revealed a mean level of 241.5 mg/l (range 145.5-506). The high specificity, sensitivity, reproducibility and accuracy of the present assay should facilitate the specific measurement of native ITI in blood and thus might represent a useful tool for further physiopathological studies.

Original languageEnglish
Pages (from-to)61-70
Number of pages10
JournalJournal of Immunological Methods
Volume190
Issue number1
DOIs
Publication statusPublished - Mar 28 1996
Externally publishedYes

Fingerprint

Trypsin Inhibitors
Enzyme-Linked Immunosorbent Assay
Antibodies
Serine Proteinase Inhibitors
Protease Inhibitors
Edetic Acid
Sensitivity and Specificity
Peptides
Enzymes

Keywords

  • ELISA
  • Inter-α-trypsin inhibitor, human

ASJC Scopus subject areas

  • Biotechnology
  • Immunology

Cite this

Development of an enzyme-linked immunosorbent assay for human plasma inter-α-trypsin inhibitor (ITI) using specific antibodies against each of the H1 and H2 heavy chains. / Mizon, C.; Balduyck, M.; Albani, D.; Michalski, C.; Burnouf, T.; Mizon, J.

In: Journal of Immunological Methods, Vol. 190, No. 1, 28.03.1996, p. 61-70.

Research output: Contribution to journalArticle

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AU - Albani, D.

AU - Michalski, C.

AU - Burnouf, T.

AU - Mizon, J.

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AB - Inter-α-trypsin inhibitor (ITI) is a serine-proteinase inhibitor of human plasma enzymes. ITI is composed of three polypeptide chains covalently linked: bikunin, responsible for the antiprotease activity and two heavy chains H1 and H2. Human plasma also contains other components immunologically related to ITI such as pre-α-trypsin inhibitor (pal), inter-α-like inhibitor (I α LI) and free bikunin. The ELISA procedure we propose exclusively measures native ITI within the range 12.5-200 μg/l. The intra- and interassay coefficients of variation were less than 5.6% and 8.7%, respectively. When ITI was added to plasma samples, full recovery was obtained, EDTA-plasma from 30 healthy individuals revealed a mean level of 241.5 mg/l (range 145.5-506). The high specificity, sensitivity, reproducibility and accuracy of the present assay should facilitate the specific measurement of native ITI in blood and thus might represent a useful tool for further physiopathological studies.

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