Development of an automated immunoassay for advanced glycosylation end products in human serum

Chuai Yu Lin, Chuan Sern Chen, Ming Song Shieh, Chih Hsiung Wu, Horng Mo Lee

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Objective: Nonenzymatic reaction of protein and carbohydrate produce a series of brown fluorescent advanced glycosylation end products (AGEs). However, a convenient and rapid assay for serum AGEs level is currently unavailable. Methods: We raised AGEs-specific polyclonal antibodies, which were used to develop a fully automated, noncompetitive, homogeneous, light-scattering immunoassay for serum AGEs. Results: The assay requires a sample volume of 2 μL and takes a reaction time of 2 min. The coefficient of variation range from 1.8 to 6.1%, and the mean recovery rate was 98.6%. Comparative analysis shows moderate correlation with competitive ELISA (r = 0.8209, n = 52). The mean ± SD concentration of AGEs in young and in older healthy subjects were 4.6 ± 1.5 (n = 39) and 4.9 ± 1.4 (n = 40), respectively. The level of AGEs was significantly higher in serum from patients with type II DM 7.8 ± 4.8 (n = 89) than that from the normal subjects (p <0.05). Conclusions: The automatic immunoassay for AGEs is appropriate for clinical use.

Original languageEnglish
Pages (from-to)189-195
Number of pages7
JournalClinical Biochemistry
Volume35
Issue number3
DOIs
Publication statusPublished - 2002

Keywords

  • Advanced glycosylation end products
  • Competitive ELISA
  • Diabetes mellitus
  • End stage renal diseases
  • Light-scattering immunoassay

ASJC Scopus subject areas

  • Clinical Biochemistry

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