Development of an anti-methoxy poly(ethylene glycol) (α-mPEG) cell-based capture system to measure mPEG and mPEGylated molecules

Kuo Hsiang Chuang, Chien Han Kao, Steve R. Roffler, Ssu Jung Lu, Ta Chun Cheng, Yun Ming Wang, Chih Hung Chuang, Yuan Chin Hsieh, Yeng Tseng Wang, Jaw Yuan Wang, Kuo Yi Weng, Tian Lu Cheng

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Quantitative pharmacokinetic analysis of methoxy-poly(ethylene glycol) (mPEG) and mPEGylated molecules is important for clinical drug development. Here we developed sensitive sandwich and competitive ELISAs by expressing an anti-mPEG antibody on the surface of fibroblasts for effective capture of mPEG molecules in biological samples. α-mPEG sandwich ELISA could quantify the higher-molecular-weight of mPEG (2, 5, and 20 kDa) and mPEGylated molecules. α-mPEG cell-based competitive ELISA was developed to measure the lower-molecular-weight of mPEG molecules (559, 750, and 1000 Da) at nanomolar levels. In addition, α-mPEG cell-based ELISA was unaffected by the presence of 10% human serum or murine serum. We further demonstrate that the α-mPEG cell-based ELISA determined similar pharmacokinetics of mPEG5K as traditional gamma counting of 131I-mPEG5K. The α-mPEG cell-based ELISA may provide an accurate, high sensitivity and easy-to-use tool for directly measuring mPEG and mPEGylated molecules in complex biological samples to accelerate the clinical development of mPEG drugs.

Original languageEnglish
Pages (from-to)6880-6888
Number of pages9
JournalMacromolecules
Volume47
Issue number19
DOIs
Publication statusPublished - Oct 14 2014

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Polyethylene glycols
Molecules
Pharmacokinetics
Molecular weight
Fibroblasts
Antibodies
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Organic Chemistry
  • Materials Chemistry
  • Polymers and Plastics
  • Inorganic Chemistry

Cite this

Development of an anti-methoxy poly(ethylene glycol) (α-mPEG) cell-based capture system to measure mPEG and mPEGylated molecules. / Chuang, Kuo Hsiang; Kao, Chien Han; Roffler, Steve R.; Lu, Ssu Jung; Cheng, Ta Chun; Wang, Yun Ming; Chuang, Chih Hung; Hsieh, Yuan Chin; Wang, Yeng Tseng; Wang, Jaw Yuan; Weng, Kuo Yi; Cheng, Tian Lu.

In: Macromolecules, Vol. 47, No. 19, 14.10.2014, p. 6880-6888.

Research output: Contribution to journalArticle

Chuang, KH, Kao, CH, Roffler, SR, Lu, SJ, Cheng, TC, Wang, YM, Chuang, CH, Hsieh, YC, Wang, YT, Wang, JY, Weng, KY & Cheng, TL 2014, 'Development of an anti-methoxy poly(ethylene glycol) (α-mPEG) cell-based capture system to measure mPEG and mPEGylated molecules', Macromolecules, vol. 47, no. 19, pp. 6880-6888. https://doi.org/10.1021/ma501156r
Chuang, Kuo Hsiang ; Kao, Chien Han ; Roffler, Steve R. ; Lu, Ssu Jung ; Cheng, Ta Chun ; Wang, Yun Ming ; Chuang, Chih Hung ; Hsieh, Yuan Chin ; Wang, Yeng Tseng ; Wang, Jaw Yuan ; Weng, Kuo Yi ; Cheng, Tian Lu. / Development of an anti-methoxy poly(ethylene glycol) (α-mPEG) cell-based capture system to measure mPEG and mPEGylated molecules. In: Macromolecules. 2014 ; Vol. 47, No. 19. pp. 6880-6888.
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