Development of a reversed-phase high-performance liquid Chromatographie method for analyzing furanocoμmarin components in citrus fruit juices and Chinese herbal medicines

Ying Ku Lin, Ming Thau Sheu, Chia Hui Huang, Hsiu O. Ho

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16 Citations (Scopus)

Abstract

A rapid and sensitive reversed-phase high performance liquid Chromatographie method for the quantitation of five furanocoumarins (bergaptol, psoralen, bergapten, 6',7'-dihydroxybergamottin, and bergamottin) is developed and validated. HPLC analysis of these five furanocoumarins is performed on a reversed-phase Inertsil ODS-2 column with a particle size of 5 μm Using only water and acetonitrile as solvents, good separation, good precision, and high accuracy are obtained for the analysis of furanocoμmarin components. This method is validated and applied to analyze the composition of five furanocoumarins in four citrus fruit juices (grapefruit, pomelo I, pomelo II, and shaddock) and ten Chinese herbal medicines (Bai-Zhi, Qiang-Huo, Du-Huo, Fang-Feng, Dang-Gui, Huang-Qin, Gan-Cao, Chen-Pi, Ge-Gen, and Yin-Chen-Hao) prepared by water decoction or an alcohol infusion. Results show that four of the five furanocoumarins (but not bergapten) are detected in grapefruit, pomelo I, and pomelo II, and the highest amount of these components is found in grapefruit juice. In the ten Chinese herbal medicines, the five furanocoumarins are not detected in Ge-Gen or Yin-Chen-Hao. The remaining herbs contain various compositions and amounts of furanocoμmarins. In general, Chinese herbal medicines prepared by the 40% ethanol infusion contain larger amounts of furanocoumarins than those prepared by hot water decoction.

Original languageEnglish
Pages (from-to)211-215
Number of pages5
JournalJournal of Chromatographic Science
Volume47
Issue number3
Publication statusPublished - Mar 2009

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Citrus fruits
Fruit juices
Liquids
Water
Ficusin
Chemical analysis
Furocoumarins
Ethanol
Particle size
Alcohols

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

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title = "Development of a reversed-phase high-performance liquid Chromatographie method for analyzing furanocoμmarin components in citrus fruit juices and Chinese herbal medicines",
abstract = "A rapid and sensitive reversed-phase high performance liquid Chromatographie method for the quantitation of five furanocoumarins (bergaptol, psoralen, bergapten, 6',7'-dihydroxybergamottin, and bergamottin) is developed and validated. HPLC analysis of these five furanocoumarins is performed on a reversed-phase Inertsil ODS-2 column with a particle size of 5 μm Using only water and acetonitrile as solvents, good separation, good precision, and high accuracy are obtained for the analysis of furanocoμmarin components. This method is validated and applied to analyze the composition of five furanocoumarins in four citrus fruit juices (grapefruit, pomelo I, pomelo II, and shaddock) and ten Chinese herbal medicines (Bai-Zhi, Qiang-Huo, Du-Huo, Fang-Feng, Dang-Gui, Huang-Qin, Gan-Cao, Chen-Pi, Ge-Gen, and Yin-Chen-Hao) prepared by water decoction or an alcohol infusion. Results show that four of the five furanocoumarins (but not bergapten) are detected in grapefruit, pomelo I, and pomelo II, and the highest amount of these components is found in grapefruit juice. In the ten Chinese herbal medicines, the five furanocoumarins are not detected in Ge-Gen or Yin-Chen-Hao. The remaining herbs contain various compositions and amounts of furanocoμmarins. In general, Chinese herbal medicines prepared by the 40{\%} ethanol infusion contain larger amounts of furanocoumarins than those prepared by hot water decoction.",
author = "Lin, {Ying Ku} and Sheu, {Ming Thau} and Huang, {Chia Hui} and Ho, {Hsiu O.}",
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T1 - Development of a reversed-phase high-performance liquid Chromatographie method for analyzing furanocoμmarin components in citrus fruit juices and Chinese herbal medicines

AU - Lin, Ying Ku

AU - Sheu, Ming Thau

AU - Huang, Chia Hui

AU - Ho, Hsiu O.

PY - 2009/3

Y1 - 2009/3

N2 - A rapid and sensitive reversed-phase high performance liquid Chromatographie method for the quantitation of five furanocoumarins (bergaptol, psoralen, bergapten, 6',7'-dihydroxybergamottin, and bergamottin) is developed and validated. HPLC analysis of these five furanocoumarins is performed on a reversed-phase Inertsil ODS-2 column with a particle size of 5 μm Using only water and acetonitrile as solvents, good separation, good precision, and high accuracy are obtained for the analysis of furanocoμmarin components. This method is validated and applied to analyze the composition of five furanocoumarins in four citrus fruit juices (grapefruit, pomelo I, pomelo II, and shaddock) and ten Chinese herbal medicines (Bai-Zhi, Qiang-Huo, Du-Huo, Fang-Feng, Dang-Gui, Huang-Qin, Gan-Cao, Chen-Pi, Ge-Gen, and Yin-Chen-Hao) prepared by water decoction or an alcohol infusion. Results show that four of the five furanocoumarins (but not bergapten) are detected in grapefruit, pomelo I, and pomelo II, and the highest amount of these components is found in grapefruit juice. In the ten Chinese herbal medicines, the five furanocoumarins are not detected in Ge-Gen or Yin-Chen-Hao. The remaining herbs contain various compositions and amounts of furanocoμmarins. In general, Chinese herbal medicines prepared by the 40% ethanol infusion contain larger amounts of furanocoumarins than those prepared by hot water decoction.

AB - A rapid and sensitive reversed-phase high performance liquid Chromatographie method for the quantitation of five furanocoumarins (bergaptol, psoralen, bergapten, 6',7'-dihydroxybergamottin, and bergamottin) is developed and validated. HPLC analysis of these five furanocoumarins is performed on a reversed-phase Inertsil ODS-2 column with a particle size of 5 μm Using only water and acetonitrile as solvents, good separation, good precision, and high accuracy are obtained for the analysis of furanocoμmarin components. This method is validated and applied to analyze the composition of five furanocoumarins in four citrus fruit juices (grapefruit, pomelo I, pomelo II, and shaddock) and ten Chinese herbal medicines (Bai-Zhi, Qiang-Huo, Du-Huo, Fang-Feng, Dang-Gui, Huang-Qin, Gan-Cao, Chen-Pi, Ge-Gen, and Yin-Chen-Hao) prepared by water decoction or an alcohol infusion. Results show that four of the five furanocoumarins (but not bergapten) are detected in grapefruit, pomelo I, and pomelo II, and the highest amount of these components is found in grapefruit juice. In the ten Chinese herbal medicines, the five furanocoumarins are not detected in Ge-Gen or Yin-Chen-Hao. The remaining herbs contain various compositions and amounts of furanocoμmarins. In general, Chinese herbal medicines prepared by the 40% ethanol infusion contain larger amounts of furanocoumarins than those prepared by hot water decoction.

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