Development of a new type of multifunctional fucoidan-based nanoparticles for anticancer drug delivery

Kun Ying Lu, Rou Li, Chun Hua Hsu, Cheng Wei Lin, Shen Chieh Chou, Min Lang Tsai, Fwu Long Mi

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Fucoidan, a sulfated marine polysaccharide, has many potential biological functions, including anticancer activity. Recently, fucoidan has been reported to target P-selectin expressed on metastatic cancer cells. Increasing research attention has been devoted to the developments of fucoidan-based nanomedicine. However, the application of traditional chitosan/fucoidan nanoparticles in anticancer drug delivery may be limited due to the deprotonation of chitosan at a pH greater than 6.5. In this study, a mutli-stimuli-responsive nanoparticle self-assembled by fucoidan and a cationic polypeptide (protamine) was developed, and their pH-/enzyme-responsive properties were characterized by circular dichroism (CD) spectroscopy, dynamic light scattering (DLS), and zeta potential analysis. Enzymatic digestion and acidic intracellular microenvironment (pH 4.5–5.5) in cancer cells triggered the release of an anticancer drug (doxorubicin) from the nanoparticles. The protamine/fucoidan complex nanoparticles with P-selectin mediated endocytosis, charge conversion and stimuli-tunable release properties showed an improved inhibitory effect against a metastatic breast cancer cell line (MDA-MB-231).

Original languageEnglish
Pages (from-to)410-420
Number of pages11
JournalCarbohydrate Polymers
Volume165
DOIs
Publication statusPublished - Jun 1 2017

Fingerprint

Drug delivery
Nanoparticles
Cells
Chitosan
Protamines
P-Selectin
Circular dichroism spectroscopy
Medical nanotechnology
Deprotonation
Polypeptides
Dynamic light scattering
Zeta potential
Polysaccharides
Enzymes
Doxorubicin
fucoidan
Peptides
Pharmaceutical Preparations
Selectins

Keywords

  • Drug delivery
  • Fucoidan
  • Metastatic cancer cells
  • Nanoparticles
  • P-selectin
  • Polypeptide

ASJC Scopus subject areas

  • Polymers and Plastics
  • Organic Chemistry
  • Materials Chemistry

Cite this

Development of a new type of multifunctional fucoidan-based nanoparticles for anticancer drug delivery. / Lu, Kun Ying; Li, Rou; Hsu, Chun Hua; Lin, Cheng Wei; Chou, Shen Chieh; Tsai, Min Lang; Mi, Fwu Long.

In: Carbohydrate Polymers, Vol. 165, 01.06.2017, p. 410-420.

Research output: Contribution to journalArticle

Lu, Kun Ying ; Li, Rou ; Hsu, Chun Hua ; Lin, Cheng Wei ; Chou, Shen Chieh ; Tsai, Min Lang ; Mi, Fwu Long. / Development of a new type of multifunctional fucoidan-based nanoparticles for anticancer drug delivery. In: Carbohydrate Polymers. 2017 ; Vol. 165. pp. 410-420.
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