Development of a new HPLC method for the simultaneous determination of ticarcillin and clavulanic acid in pharmaceutical formulations

Tai Li Tsou, Chiu Wey Lee, Hsian-Jenn Wang, Ya Chung Cheng, Yu Tien Liu, Su Hwei Chen

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A new HPLC method has been developed and validated for the simultaneous determination of ticarcillin (TIC) and clavulanic acid (CA) in pharmaceutical formulations. The HPLC separation was achieved on a β-cyclodextrin column (Cyclobond I, 250 × 4.6 mm, 5 μm) with methanol-16 mM pH 6.0 ammonium acetate buffer (50 + 50, v/v) mobile phase at a flow rate of 0.8 mL/min. Detection was at 220 nm. Validation of the method was performed by evaluating specificity, robustness, accuracy, and precision. The calibration curves were linear in the range of 1-100 μg/mL for CA and 2-200 μg/mL for TIC. The LOQs based on the standard regression lines were 0.42 and 1.42 μg/mL for CA and TIC, respectively, and the LOD were 0.14 and 0.47 μg/mL, respectively. Total recoveries of synthetic mixtures (CA:TIC = 1:10, 1:15, and 1:30) were 99.25-100.99% for CA and 99.54-100.82% for TIC. Compared with the U.S. Pharmacopeia method, the proposed method has the advantage of a relatively low flow rate and short analysis time. The proposed method was successfully applied for the simultaneous determination of these two drugs in sterilized H 2O and 5% dextrose injection solutions.

Original languageEnglish
Pages (from-to)1089-1094
Number of pages6
JournalJournal of AOAC International
Volume92
Issue number4
Publication statusPublished - Jul 1 2009

Fingerprint

ticarcillin
drug formulations
clavulanic acid
Drug Compounding
Ticarcillin
Clavulanic Acid
drug
high performance liquid chromatography
High Pressure Liquid Chromatography
acid
Flow rate
Pharmaceutical Preparations
Cyclodextrins
Methanol
Buffers
methodology
ammonium acetate
Pharmacopoeias
cyclodextrins
Calibration

ASJC Scopus subject areas

  • Analytical Chemistry
  • Food Science
  • Environmental Chemistry
  • Agronomy and Crop Science
  • Pharmacology

Cite this

Development of a new HPLC method for the simultaneous determination of ticarcillin and clavulanic acid in pharmaceutical formulations. / Tsou, Tai Li; Lee, Chiu Wey; Wang, Hsian-Jenn; Cheng, Ya Chung; Liu, Yu Tien; Chen, Su Hwei.

In: Journal of AOAC International, Vol. 92, No. 4, 01.07.2009, p. 1089-1094.

Research output: Contribution to journalArticle

Tsou, Tai Li ; Lee, Chiu Wey ; Wang, Hsian-Jenn ; Cheng, Ya Chung ; Liu, Yu Tien ; Chen, Su Hwei. / Development of a new HPLC method for the simultaneous determination of ticarcillin and clavulanic acid in pharmaceutical formulations. In: Journal of AOAC International. 2009 ; Vol. 92, No. 4. pp. 1089-1094.
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