Development of a biochip using antibody-coated gold nanoparticles to detect specific bioparticles

Jung Tang Huang, Shao Y. Hou, Shiuh B. Fang, Hua W. Yu, Hung Chan Lee, Chu Z. Yang

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

This study developed a method of detecting bioparticles such as Salmonella that exist in the biological samples. The method employed a substrate with interlaced comb-like electrodes into which the mixtures of biological samples and antibody-coated gold nanoparticles were added. The alternative signals with appropriate frequency bands were then conducted into the comb-like electrodes to change the dielectrophoresis force. The gold-modified Salmonella can be adsorbed on the edges of the electrodes and isolated from various biological samples. The impedance of the adsorbed Salmonella on the edges of the electrodes was measured and comparison of the impedance between the electrodes with and without Salmonella can quantify the amount of the adsorbed Salmonella.

Original languageEnglish
Pages (from-to)1377-1385
Number of pages9
JournalJournal of Industrial Microbiology and Biotechnology
Volume35
Issue number11
DOIs
Publication statusPublished - Nov 2008
Externally publishedYes

Keywords

  • Biochip
  • Detection
  • Dielectrophoresis
  • Gold nanoparticles
  • Salmonella

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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