Development and characterization of a bioinspired bone matrix with aligned nanocrystalline hydroxyapatite on collagen nanofibers

Hsi Chin Wu, Tzu Wei Wang, Jui Sheng Sun, Yi Hsuan Lee, Meng Han Shen, Zong Ruei Tsai, Chih Yu Chen, Horng Chaung Hsu

Research output: Contribution to journalArticle

14 Citations (Scopus)


Various kinds of three-dimensional (3D) scaffolds have been designed to mimic the biological spontaneous bone formation characteristics by providing a suitable microenvironment for osteogenesis. In view of this, a natural bone-liked composite scaffold, which was combined with inorganic (hydroxyapatite, Hap) and organic (type I collagen, Col) phases, has been developed through a self-assembly process. This 3D porous scaffold consisting of a c-axis of Hap nanocrystals (nHap) aligning along Col fibrils arrangement is similar to natural bone architecture. A significant increase in mechanical strength and elastic modulus of nHap/Col scaffold is achieved through biomimetic mineralization process when compared with simple mixture of collagen and hydroxyapatite method. It is suggested that the self-organization of Hap and Col produced in vivo could also be achieved in vitro. The oriented nHap/Col composite not only possesses bone-like microstructure and adequate mechanical properties but also enhances the regeneration and reorganization abilities of bone tissue. These results demonstrated that biomimetic nHap/Col can be successfully reconstructed as a bone graft substitute in bone tissue engineering.

Original languageEnglish
Article number198
Issue number3
Publication statusPublished - Mar 15 2016
Externally publishedYes



  • Biomimetic scaffold
  • Hydroxyapatite nanocrystal
  • Nanocomposite

ASJC Scopus subject areas

  • Materials Science(all)

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