Developement of valve-integrated nanofluidic preconcentrator for low-abundance protein detection

Chih Zong Deng, Yu Jui Fan, Horn Jiunn Sheen

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this study, we developed a new immunosensor that can achieve ultra-low detection limit and high sensitivity. This new device has an electrokinetic trapping (EKT)-based nanofluidic preconcentrator, which was integrated with oscillating bubble valves, to trap concentrated antigen and immunobeads. During the immunoassay process, oscillating bubbles rapidly grew and acted as control valves and to block the microchannel. Thereafter, the trapped preconcentrated antigen plug and antibody-coated nanobeads were preserved in the region between these two valves. Finally, the antigen concentration was quantitatively analyzed by a real-time measurement of Brownian diffusion of the immuno-beads. In this work, the test sample used was C-reactive protein (CRP) which is a risk indicator of coronary heart dis-ease and atherosclerosis.

Original languageEnglish
Title of host publication22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
PublisherChemical and Biological Microsystems Society
Pages853-856
Number of pages4
ISBN (Electronic)9781510897571
Publication statusPublished - Jan 1 2018
Event22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 - Kaohsiung, Taiwan
Duration: Nov 11 2018Nov 15 2018

Publication series

Name22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Volume2

Conference

Conference22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
CountryTaiwan
CityKaohsiung
Period11/11/1811/15/18

Keywords

  • Formatting
  • Layout
  • Manuscript
  • PDF-file

ASJC Scopus subject areas

  • Chemistry(all)
  • Bioengineering
  • Chemical Engineering (miscellaneous)
  • Control and Systems Engineering

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  • Cite this

    Deng, C. Z., Fan, Y. J., & Sheen, H. J. (2018). Developement of valve-integrated nanofluidic preconcentrator for low-abundance protein detection. In 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 (pp. 853-856). (22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018; Vol. 2). Chemical and Biological Microsystems Society.