Determination of the components in a Chinese prescription, Yu-Ping-Feng San, by RAPD analysis

Kur Ta Cheng, Hsin Sheng Tsay, Chieh Fu Chen, Tsue Wei Chou

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

In this study, the RAPD (random amplified polymorphic DNA) technique was employed for the first time to determine the components in a Chinese herbal prescription. Forty decamer oligonucleotide primers were screened in the RAPD analysis to identify three Chinese medicines, the dried root of Astragalus membranaceus (Fisch.) Bge., the dried root of Ledebouriella seseloides Wolff, and the dried rhizome of Atractylodes macrocephala Koidz, in a Chinese prescription. Only primer OPP-10 simultaneously generated three distinct markers were each specific to one component. The marker with 200 bp is specific to Astragalus membranaceus; the 440 bp marker is specific to Atractylodes macrocephala; and the remaining marker with 500 bp was present in Ledebouriella seseloides. The presence of the three herbal medicines in the mixed sample, the Chinese prescription, was determined when the primer OPP-10 RAPD reaction was performed. The technique was proved to contribute to the identification of components in the ChineSe medicinal preparations.

Original languageEnglish
Pages (from-to)563-565
Number of pages3
JournalPlanta Medica
Volume64
Issue number6
Publication statusPublished - Aug 1998

Keywords

  • Astragalus membronaceus
  • Atractylodes macrocephala
  • Chinese herbal prescription
  • Identification
  • Ledebouriella seseloides
  • RAPD

ASJC Scopus subject areas

  • Plant Science
  • Drug Discovery
  • Organic Chemistry
  • Pharmacology

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