Determination of sesamin and sesamolin by using HPLC with an internal standard

Wei Ju Lee, Poi Yik Teh, Nan Wei Su, Min Hsiung Lee

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Major lignans in sesame seeds are sesamin and sesamolin. Numerous studies have reported that these lignans have physiological functions. However, there are few researches regarding the methods for quantification of sesamin and sesamolin. Thus, the results varied significantly due to the different quantitative methods used in reported studies. In the present study, we used naphthalene as an internal standard to establish an HPLC method to quantify the contents of sesamin and sesamolin. The results of validation experiments indicated that this quantitative method developed in this study could provide a reasonable method for determination of sesamin and sesamolin with high recoveries, accuracy and precision. It takes 25 minutes to finish one analysis. The LOQ of sesamin is 75 μg/L, and the LOQ of sesamolin is 150 μg/L. We also used this method to determine the sesamin and sesamolin contents of the commercial products, and compared with their labeled amounts. This method could be a reference for the industry and authorities to regulate the lignan contents in sesame-related commercial products.

Original languageEnglish
Pages (from-to)102-108
Number of pages7
JournalTaiwanese Journal of Agricultural Chemistry and Food Science
Volume51
Issue number2
Publication statusPublished - Jan 1 2013
Externally publishedYes

Fingerprint

lignans
High Pressure Liquid Chromatography
Lignans
quantitative analysis
Sesamum
sesame seed
Sesamum indicum
naphthalene
research methods
methodology
industry
sesamin
sesamolin
Seeds
Industry
Research

Keywords

  • Lignans
  • Naphthalene
  • Sesame seeds
  • Sesamin
  • Sesamolin

ASJC Scopus subject areas

  • Food Science
  • Applied Microbiology and Biotechnology
  • Agronomy and Crop Science

Cite this

Determination of sesamin and sesamolin by using HPLC with an internal standard. / Lee, Wei Ju; Teh, Poi Yik; Su, Nan Wei; Lee, Min Hsiung.

In: Taiwanese Journal of Agricultural Chemistry and Food Science, Vol. 51, No. 2, 01.01.2013, p. 102-108.

Research output: Contribution to journalArticle

@article{6a32dfb05e7f4943be7ef58eb5b5b7ce,
title = "Determination of sesamin and sesamolin by using HPLC with an internal standard",
abstract = "Major lignans in sesame seeds are sesamin and sesamolin. Numerous studies have reported that these lignans have physiological functions. However, there are few researches regarding the methods for quantification of sesamin and sesamolin. Thus, the results varied significantly due to the different quantitative methods used in reported studies. In the present study, we used naphthalene as an internal standard to establish an HPLC method to quantify the contents of sesamin and sesamolin. The results of validation experiments indicated that this quantitative method developed in this study could provide a reasonable method for determination of sesamin and sesamolin with high recoveries, accuracy and precision. It takes 25 minutes to finish one analysis. The LOQ of sesamin is 75 μg/L, and the LOQ of sesamolin is 150 μg/L. We also used this method to determine the sesamin and sesamolin contents of the commercial products, and compared with their labeled amounts. This method could be a reference for the industry and authorities to regulate the lignan contents in sesame-related commercial products.",
keywords = "Lignans, Naphthalene, Sesame seeds, Sesamin, Sesamolin",
author = "Lee, {Wei Ju} and Teh, {Poi Yik} and Su, {Nan Wei} and Lee, {Min Hsiung}",
year = "2013",
month = "1",
day = "1",
language = "English",
volume = "51",
pages = "102--108",
journal = "Taiwanese Journal of Agricultural Chemistry and Food Science",
issn = "1605-2471",
publisher = "Chinese Agricultural Chemical Society",
number = "2",

}

TY - JOUR

T1 - Determination of sesamin and sesamolin by using HPLC with an internal standard

AU - Lee, Wei Ju

AU - Teh, Poi Yik

AU - Su, Nan Wei

AU - Lee, Min Hsiung

PY - 2013/1/1

Y1 - 2013/1/1

N2 - Major lignans in sesame seeds are sesamin and sesamolin. Numerous studies have reported that these lignans have physiological functions. However, there are few researches regarding the methods for quantification of sesamin and sesamolin. Thus, the results varied significantly due to the different quantitative methods used in reported studies. In the present study, we used naphthalene as an internal standard to establish an HPLC method to quantify the contents of sesamin and sesamolin. The results of validation experiments indicated that this quantitative method developed in this study could provide a reasonable method for determination of sesamin and sesamolin with high recoveries, accuracy and precision. It takes 25 minutes to finish one analysis. The LOQ of sesamin is 75 μg/L, and the LOQ of sesamolin is 150 μg/L. We also used this method to determine the sesamin and sesamolin contents of the commercial products, and compared with their labeled amounts. This method could be a reference for the industry and authorities to regulate the lignan contents in sesame-related commercial products.

AB - Major lignans in sesame seeds are sesamin and sesamolin. Numerous studies have reported that these lignans have physiological functions. However, there are few researches regarding the methods for quantification of sesamin and sesamolin. Thus, the results varied significantly due to the different quantitative methods used in reported studies. In the present study, we used naphthalene as an internal standard to establish an HPLC method to quantify the contents of sesamin and sesamolin. The results of validation experiments indicated that this quantitative method developed in this study could provide a reasonable method for determination of sesamin and sesamolin with high recoveries, accuracy and precision. It takes 25 minutes to finish one analysis. The LOQ of sesamin is 75 μg/L, and the LOQ of sesamolin is 150 μg/L. We also used this method to determine the sesamin and sesamolin contents of the commercial products, and compared with their labeled amounts. This method could be a reference for the industry and authorities to regulate the lignan contents in sesame-related commercial products.

KW - Lignans

KW - Naphthalene

KW - Sesame seeds

KW - Sesamin

KW - Sesamolin

UR - http://www.scopus.com/inward/record.url?scp=84901442797&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84901442797&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:84901442797

VL - 51

SP - 102

EP - 108

JO - Taiwanese Journal of Agricultural Chemistry and Food Science

JF - Taiwanese Journal of Agricultural Chemistry and Food Science

SN - 1605-2471

IS - 2

ER -