Determination of nimetazepam and 7-aminonimetazepam in human urine by using liquid chromatography-tandem mass spectrometry

Kuang Chuan Wang, Min Chi Cheng, Chin Lin Hsieh, Jung Fa Hsu, Jen Der Wu, Ching Kuo Lee

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

We report determination of metabolites of popular drugs of abuse, including nimetazepam and nitrazepam, in urine by using liquid chromatography/mass spectrometry. Nimetazepam and its metabolites, 7-aminonimetazepam and nitrazepam, were extracted by solid-phase extraction using a DAU cartridge. An ammonium acetate buffer solution (pH 4) and a Luna polar-RP column were selected as the mobile and stationary phase, respectively, for liquid chromatography. Mass spectrometry was used for analysis and was optimized for operation in the positive mode for all analytes. The urine specimens were screened for the presence of nimetazepam and its metabolites nitrazepam and 7-aminonimetazepam at a concentration of 0.1. ng/mL. Presence of 7-aminonimetazepam in the urine was an indicator of the subject being a probable abuser of nimetazepam.

Original languageEnglish
Pages (from-to)84-89
Number of pages6
JournalForensic Science International
Volume224
Issue number1-3
DOIs
Publication statusPublished - Jan 10 2013

Keywords

  • 7-Aminonimetazepam
  • LC-MS
  • Nimetazepam
  • Nitrazepam

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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