Determination of nalbuphine by high-performance liquid chromatography with ultraviolet detection: Application to human and rabbit pharmacokinetic studies

Shung Tai Ho, Jhi Joung Wang, Oliver Yoa Pu Hu, Pei Shan Chiang, Shih Chun Lee

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

A rapid, sensitive, precise and accurate high-performance liquid chromatographic assay with ultraviolet detection was developed for the determination of nalbuphine in human, rabbit, pig and dog plasma. It is comprised of only a one-step extraction procedure with hexane-isoamyl alcohol at pH 9.25 and reversed-phase chromatography on a μPorasil column. The recoveries of nalbuphine and ethylmorphine (internal standard) were greater than 86%. Calibration graphs were linear over the concentration range 0.75-150 ng/ml with a coefficient of variation, both within-day and between-day, of less than 10% at any level. The limit of quantitation was 0.75 ng/ml of plasma based on a signal-to-noise ratio of 3. Seven other clinically used analgesics were investigated to check for potential interferences and their analytical conditions. The specificity of this assay was checked with a metabolite of nalbuphine (noroxymorphine). Nalbuphine in plasma did not decompose significantly at -20°C for six weeks. Pharmacokinetic application in three surgical patients and four rabbits revealed that nalbuphine followed a linear three-compartment model with two distribution phases. The two distribution and one elimination half-lives and the plasma clearance of nalbuphine were 0.9, 5.8 and 157 min and 370 ml/min in human, and 3.5, 28 and 117 min and 21 166 ml/min in rabbits.

Original languageEnglish
Pages (from-to)289-296
Number of pages8
JournalJournal of Chromatography B: Biomedical Applications
Volume678
Issue number2
DOIs
Publication statusPublished - Apr 12 1996
Externally publishedYes

Fingerprint

Nalbuphine
Pharmacokinetics
High performance liquid chromatography
Plasmas
Assays
Ethylmorphine
Hexanes
Metabolites
Chromatography
Analgesics
Signal to noise ratio
Calibration
Recovery
Liquids

Keywords

  • Nalbuphine

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Determination of nalbuphine by high-performance liquid chromatography with ultraviolet detection : Application to human and rabbit pharmacokinetic studies. / Ho, Shung Tai; Wang, Jhi Joung; Hu, Oliver Yoa Pu; Chiang, Pei Shan; Lee, Shih Chun.

In: Journal of Chromatography B: Biomedical Applications, Vol. 678, No. 2, 12.04.1996, p. 289-296.

Research output: Contribution to journalArticle

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