Determination of duffy genotypes in three populations of African descent using PCR and sequence-specific oligonucleotides

Renate G. Nickel, Stephanie Ann Willadsen, Linda R. Freidhoff, Shau Ku Huang, Luis Caraballo, Raana P. Naidu, Paul Levett, Malcolm Blumenthal, Susan Banks-Schlegel, Eugene Bleecker, Terri Beaty, Carole Ober, Kathleen C. Barnes

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The expression of the Duffy Antigen/Receptor for Chemokines (DARC) on red blood cells (RBC) has been commonly determined using hemagglutination tests. Because the vast majority of African individuals are Duffy-negative, screening for DARC expression on RBC is a valuable tool to assess Caucasian admixture in populations of African descent. Furthermore, blood group antigens have been frequently rested as potential risk factors for complex diseases. We established a dot-blotting protocol using sequence-specific oligonucleotides (SSOs) for tile DARC-46T ('Duffy-positive') and -46C ('Duffy-negative') alleles. With this method, but nor with serological methods, Duffy-positive individuals can be further characterized as homozygous or heterozygous for the dominant Duffy-positive allele, allowing more precise estimation of allele frequencies anti admixture in heterogeneous populations. In unrelated African American (n = 235), Afro-Caribbean (n = 90) and Colombian (n = 93) subjects, the frequency of the -46T allele was 21.7%, 12.2% and 74.7%, respectively. The percentage of Duffy-positive individuals (homozygous or heterozygous for the -46T allele) in each population was in accordance with published frequencies. As expected, the -46C allele was nor detected in 20 Caucasian subjects. This sensitive and specific method allows for the rapid and inexpensive screening of large samples for Duffy genotypes using small quantities of genomic DNA.

Original languageEnglish
Pages (from-to)738-742
Number of pages5
JournalHuman Immunology
Volume60
Issue number8
DOIs
Publication statusPublished - Aug 1 1999
Externally publishedYes

Fingerprint

Oligonucleotides
Antigen Receptors
Alleles
Genotype
Chemokines
Polymerase Chain Reaction
Gene Frequency
Population
Erythrocytes
Hemagglutination Tests
Blood Group Antigens
African Americans
DNA

Keywords

  • DARC
  • Duffy
  • SSO

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Determination of duffy genotypes in three populations of African descent using PCR and sequence-specific oligonucleotides. / Nickel, Renate G.; Willadsen, Stephanie Ann; Freidhoff, Linda R.; Huang, Shau Ku; Caraballo, Luis; Naidu, Raana P.; Levett, Paul; Blumenthal, Malcolm; Banks-Schlegel, Susan; Bleecker, Eugene; Beaty, Terri; Ober, Carole; Barnes, Kathleen C.

In: Human Immunology, Vol. 60, No. 8, 01.08.1999, p. 738-742.

Research output: Contribution to journalArticle

Nickel, RG, Willadsen, SA, Freidhoff, LR, Huang, SK, Caraballo, L, Naidu, RP, Levett, P, Blumenthal, M, Banks-Schlegel, S, Bleecker, E, Beaty, T, Ober, C & Barnes, KC 1999, 'Determination of duffy genotypes in three populations of African descent using PCR and sequence-specific oligonucleotides', Human Immunology, vol. 60, no. 8, pp. 738-742. https://doi.org/10.1016/S0198-8859(99)00039-7
Nickel, Renate G. ; Willadsen, Stephanie Ann ; Freidhoff, Linda R. ; Huang, Shau Ku ; Caraballo, Luis ; Naidu, Raana P. ; Levett, Paul ; Blumenthal, Malcolm ; Banks-Schlegel, Susan ; Bleecker, Eugene ; Beaty, Terri ; Ober, Carole ; Barnes, Kathleen C. / Determination of duffy genotypes in three populations of African descent using PCR and sequence-specific oligonucleotides. In: Human Immunology. 1999 ; Vol. 60, No. 8. pp. 738-742.
@article{b694d15d0f9e4e9fb9ca0746fba0e370,
title = "Determination of duffy genotypes in three populations of African descent using PCR and sequence-specific oligonucleotides",
abstract = "The expression of the Duffy Antigen/Receptor for Chemokines (DARC) on red blood cells (RBC) has been commonly determined using hemagglutination tests. Because the vast majority of African individuals are Duffy-negative, screening for DARC expression on RBC is a valuable tool to assess Caucasian admixture in populations of African descent. Furthermore, blood group antigens have been frequently rested as potential risk factors for complex diseases. We established a dot-blotting protocol using sequence-specific oligonucleotides (SSOs) for tile DARC-46T ('Duffy-positive') and -46C ('Duffy-negative') alleles. With this method, but nor with serological methods, Duffy-positive individuals can be further characterized as homozygous or heterozygous for the dominant Duffy-positive allele, allowing more precise estimation of allele frequencies anti admixture in heterogeneous populations. In unrelated African American (n = 235), Afro-Caribbean (n = 90) and Colombian (n = 93) subjects, the frequency of the -46T allele was 21.7{\%}, 12.2{\%} and 74.7{\%}, respectively. The percentage of Duffy-positive individuals (homozygous or heterozygous for the -46T allele) in each population was in accordance with published frequencies. As expected, the -46C allele was nor detected in 20 Caucasian subjects. This sensitive and specific method allows for the rapid and inexpensive screening of large samples for Duffy genotypes using small quantities of genomic DNA.",
keywords = "DARC, Duffy, SSO",
author = "Nickel, {Renate G.} and Willadsen, {Stephanie Ann} and Freidhoff, {Linda R.} and Huang, {Shau Ku} and Luis Caraballo and Naidu, {Raana P.} and Paul Levett and Malcolm Blumenthal and Susan Banks-Schlegel and Eugene Bleecker and Terri Beaty and Carole Ober and Barnes, {Kathleen C.}",
year = "1999",
month = "8",
day = "1",
doi = "10.1016/S0198-8859(99)00039-7",
language = "English",
volume = "60",
pages = "738--742",
journal = "Human Immunology",
issn = "0198-8859",
publisher = "Elsevier Inc.",
number = "8",

}

TY - JOUR

T1 - Determination of duffy genotypes in three populations of African descent using PCR and sequence-specific oligonucleotides

AU - Nickel, Renate G.

AU - Willadsen, Stephanie Ann

AU - Freidhoff, Linda R.

AU - Huang, Shau Ku

AU - Caraballo, Luis

AU - Naidu, Raana P.

AU - Levett, Paul

AU - Blumenthal, Malcolm

AU - Banks-Schlegel, Susan

AU - Bleecker, Eugene

AU - Beaty, Terri

AU - Ober, Carole

AU - Barnes, Kathleen C.

PY - 1999/8/1

Y1 - 1999/8/1

N2 - The expression of the Duffy Antigen/Receptor for Chemokines (DARC) on red blood cells (RBC) has been commonly determined using hemagglutination tests. Because the vast majority of African individuals are Duffy-negative, screening for DARC expression on RBC is a valuable tool to assess Caucasian admixture in populations of African descent. Furthermore, blood group antigens have been frequently rested as potential risk factors for complex diseases. We established a dot-blotting protocol using sequence-specific oligonucleotides (SSOs) for tile DARC-46T ('Duffy-positive') and -46C ('Duffy-negative') alleles. With this method, but nor with serological methods, Duffy-positive individuals can be further characterized as homozygous or heterozygous for the dominant Duffy-positive allele, allowing more precise estimation of allele frequencies anti admixture in heterogeneous populations. In unrelated African American (n = 235), Afro-Caribbean (n = 90) and Colombian (n = 93) subjects, the frequency of the -46T allele was 21.7%, 12.2% and 74.7%, respectively. The percentage of Duffy-positive individuals (homozygous or heterozygous for the -46T allele) in each population was in accordance with published frequencies. As expected, the -46C allele was nor detected in 20 Caucasian subjects. This sensitive and specific method allows for the rapid and inexpensive screening of large samples for Duffy genotypes using small quantities of genomic DNA.

AB - The expression of the Duffy Antigen/Receptor for Chemokines (DARC) on red blood cells (RBC) has been commonly determined using hemagglutination tests. Because the vast majority of African individuals are Duffy-negative, screening for DARC expression on RBC is a valuable tool to assess Caucasian admixture in populations of African descent. Furthermore, blood group antigens have been frequently rested as potential risk factors for complex diseases. We established a dot-blotting protocol using sequence-specific oligonucleotides (SSOs) for tile DARC-46T ('Duffy-positive') and -46C ('Duffy-negative') alleles. With this method, but nor with serological methods, Duffy-positive individuals can be further characterized as homozygous or heterozygous for the dominant Duffy-positive allele, allowing more precise estimation of allele frequencies anti admixture in heterogeneous populations. In unrelated African American (n = 235), Afro-Caribbean (n = 90) and Colombian (n = 93) subjects, the frequency of the -46T allele was 21.7%, 12.2% and 74.7%, respectively. The percentage of Duffy-positive individuals (homozygous or heterozygous for the -46T allele) in each population was in accordance with published frequencies. As expected, the -46C allele was nor detected in 20 Caucasian subjects. This sensitive and specific method allows for the rapid and inexpensive screening of large samples for Duffy genotypes using small quantities of genomic DNA.

KW - DARC

KW - Duffy

KW - SSO

UR - http://www.scopus.com/inward/record.url?scp=0032800028&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032800028&partnerID=8YFLogxK

U2 - 10.1016/S0198-8859(99)00039-7

DO - 10.1016/S0198-8859(99)00039-7

M3 - Article

VL - 60

SP - 738

EP - 742

JO - Human Immunology

JF - Human Immunology

SN - 0198-8859

IS - 8

ER -