Determination of citrus juice coumarins, furanocoumarins and methoxylated flavones using solid phase extraction and HPLC with photodiode array and fluorescence detection

Gui Jie Li, Hou Jiu Wu, Yu Wang, Wei Lun Hung, Russell L. Rouseff

Research output: Contribution to journalArticle

  • 1 Citations

Abstract

A synergistic combination of analytical techniques was developed for the simultaneous determination of the three most biologically active chemical families in citrus juices: methoxylated flavones, coumarins, and furanocoumarins. No rapid methodology has been available to determine them together. A solid phase extraction concentrated these groups and a ternary reverse phase HPLC gradient completely resolved them from other juice components. Two coumarins, isomeranzin and osthole, were identified in a sweet orange (C. sinensis) cultivar, Changyecheng, for the first time. Pummelo juice was characterized by coumarin and furanocoumarin epoxides such as meranzin and epoxybergamottin. No epoxides were observed in the more acidic juices. Added furanocoumarin epoxides hydrolyzed rapidly in the most acidic juices. The ratios of the UV peak areas at 320 nm to the fluorescence emission peaks as well as the ratio of fluorescence emission peaks at 450–400 nm could be used to identify chromatographic peaks.

LanguageEnglish
Pages29-38
Number of pages10
JournalFood Chemistry
Volume271
DOIs
Publication statusPublished - Jan 15 2019
Externally publishedYes

Fingerprint

citrus juices
Flavones
Coumarins
psoralens
coumarins
Citrus
Epoxy Compounds
Solid Phase Extraction
flavones
solid phase extraction
Photodiodes
juices
high performance liquid chromatography
Fluorescence
epoxides
High Pressure Liquid Chromatography
fluorescence
coumarin
analytical methods
Furocoumarins

Keywords

  • 6′,7′-Dihydroxybergamottin (PubChem CID: 6440498)
  • Citropten (PubChem CID: 2775)
  • Epoxybergamottin (PubChem CID: 9946625)
  • Fully methoxylated flavone
  • Furocoumarin
  • Isomeranzin (PubChem CID: 473252)
  • Meranzin (PubChem CID: 1803558)
  • Nobiletin (PubChem CID: 72344)
  • Osthole (PubChem CID: 10228)
  • Oxypeucedanin (PubChem CID: 160544)
  • Polymethoxyflavones
  • Psoralen
  • Sample preparation
  • Scopoletin (PubChem CID: 5280460)
  • Sinensetin (PubChem CID: 145659)

ASJC Scopus subject areas

  • Analytical Chemistry
  • Food Science

Cite this

Determination of citrus juice coumarins, furanocoumarins and methoxylated flavones using solid phase extraction and HPLC with photodiode array and fluorescence detection. / Li, Gui Jie; Wu, Hou Jiu; Wang, Yu; Hung, Wei Lun; Rouseff, Russell L.

In: Food Chemistry, Vol. 271, 15.01.2019, p. 29-38.

Research output: Contribution to journalArticle

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abstract = "A synergistic combination of analytical techniques was developed for the simultaneous determination of the three most biologically active chemical families in citrus juices: methoxylated flavones, coumarins, and furanocoumarins. No rapid methodology has been available to determine them together. A solid phase extraction concentrated these groups and a ternary reverse phase HPLC gradient completely resolved them from other juice components. Two coumarins, isomeranzin and osthole, were identified in a sweet orange (C. sinensis) cultivar, Changyecheng, for the first time. Pummelo juice was characterized by coumarin and furanocoumarin epoxides such as meranzin and epoxybergamottin. No epoxides were observed in the more acidic juices. Added furanocoumarin epoxides hydrolyzed rapidly in the most acidic juices. The ratios of the UV peak areas at 320 nm to the fluorescence emission peaks as well as the ratio of fluorescence emission peaks at 450–400 nm could be used to identify chromatographic peaks.",
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