Determination of caffeic acid in rabbit plasma by high-performance liquid chromatography

Yow Shieng Uang, Fang Lien Kang, Kuang Yang Hsu

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21 Citations (Scopus)

Abstract

A simple and sensitive high-performance liquid chromatographic method involving UV detection was developed for determination of caffeic acid in rabbit plasma. A Lichrosphere CN column (250 mm × 4 mm I.D., 5 μm) was used as the stationary phase and the mobile phase consisted of 2% acetic acid solution at a flow-rate of 1.0 ml/min. The UV absorbance was monitored at 320 nm. The plasma sample was acidified by the addition of 0.01 parts of concentrated phosphoric acid (85%) to maintain caffeic acid stability. After a simple clean-up procedure, the limit of quantitation achieved was 0.1 μg/ml, and the standard curve was found to be linear over the concentration ranges of 0.1-2.0 μg/ml and 0.1-40 μg/ml. The coefficient of variation for within- and between-run precision and accuracy was less than 10%, and the recovery was 82.3%.

Original languageEnglish
Pages (from-to)43-49
Number of pages7
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume673
Issue number1
DOIs
Publication statusPublished - Nov 3 1995

ASJC Scopus subject areas

  • Chemistry(all)

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