Determination and pharmacokinetics of Di-(2-ethylhexyl) phthalate in rats by ultra performance liquid chromatography with tandem mass spectrometry

Wan Ling Chang-Liao, Mei Ling Hou, Li Wen Chang, Chia Jung Lee, Yin Meng Tsai, Lie Chwen Lin, Tung Hu Tsai

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11 Citations (Scopus)


Di-(2-ethylhexyl) phthalate (DEHP) is used to increase the flexibility of plastics for industrial products. However, the illegal use of the plasticizer DEHP in food and drinks has been reported in Taiwan in 2011. In order to assess the exact extent of the absorption of DEHP via the oral route, the aim of this study is to develop a reliable and validated ultra performance liquid chromatography with tandem mass spectrometry (UPLC-MS/MS) method to evaluate the oral bioavailability of DEHP in rats. The optimal chromatographic separation of DEHP and butyl benzyl phthalate (BBP; used as internal standard) were achieved on a C18 column. The mobile phase was consisted of 5 mM ammonium acetatemethanol (11:89, v/v) with a flow rate of 0.25 mL/min. The monitoring ion transitions were m/z 391.4 → 149.0 for DEHP and m/z 313.3 → 149.0 for BBP. The mean matrix effects of DEHP at low, medium and high concentrations were 94.5 ± 5.7% and 100.1 ± 2.3% in plasma and feces homogenate samples, respectively. In conclusion, the validated UPLCMS/ MS method is suitable for analyzing the rat plasma sample of DEHP and the oral bioavailability of DEHP was about 7% in rats.

Original languageEnglish
Pages (from-to)11452-11466
Number of pages15
Issue number9
Publication statusPublished - Sep 2013
Externally publishedYes



  • Di-(2-ethylhexyl) phthalate (DEHP)
  • Fecal excretion
  • Pharmacokinetics
  • Phthalates
  • Plasticizer

ASJC Scopus subject areas

  • Organic Chemistry
  • Medicine(all)

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