Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in human serum using a localized surface plasmon coupled fluorescence fiber-optic biosensor

Jason C. Huang, Ying Feng Chang, Kuan Hsuan Chen, Li Chen Su, Chun Wei Lee, Chii Chang Chen, Yi Ming Arthur Chen, Chien Chou

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

In order to enhance the sensitivity of conventional immunoassay technology for the detection of SARS coronavirus (SARS-CoV) nucleocapsid protein (N protein), we developed a localized surface plasmon coupled fluorescence (LSPCF) fiber-optic biosensor that combines sandwich immunoassay with the LSP technique. Experimentally, a linear relationship between the fluorescence signal and the concentration of recombinant SARS-CoV N (GST-N) protein in buffer solution could be observed from 0.1 pg/mL to 1 ng/mL. In addition, the concentration of GST-N protein in diluted serum across a similar range could also be measured. The correlation coefficients (linear scale) for these two measurements were 0.9469 and 0.9624, respectively. In comparison with conventional enzyme linked immunosorbent assay (ELISA), the detection limit of the LSPCF fiber-optic biosensor for the GST-N protein was improved at least 104-fold using the same monoclonal antibodies. Therefore, the LSPCF fiber-optic biosensor shows an ability to detect very low concentration (∼1 pg/mL) of SARS-CoV N protein in serum. The biosensor should help with the early diagnosis of SARS infection.

Original languageEnglish
Pages (from-to)320-325
Number of pages6
JournalBiosensors and Bioelectronics
Volume25
Issue number2
DOIs
Publication statusPublished - Oct 15 2009
Externally publishedYes

Keywords

  • Fiber-optic biosensor
  • Gold nanoparticle
  • Localized surface plasmon
  • Nucleocapsid protein
  • Severe acute respiratory syndrome (SARS)

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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