Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in human serum using a localized surface plasmon coupled fluorescence fiber-optic biosensor

Jason C. Huang, Ying Feng Chang, Kuan Hsuan Chen, Li Chen Su, Chun Wei Lee, Chii Chang Chen, Yi Ming Arthur Chen, Chien Chou

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

In order to enhance the sensitivity of conventional immunoassay technology for the detection of SARS coronavirus (SARS-CoV) nucleocapsid protein (N protein), we developed a localized surface plasmon coupled fluorescence (LSPCF) fiber-optic biosensor that combines sandwich immunoassay with the LSP technique. Experimentally, a linear relationship between the fluorescence signal and the concentration of recombinant SARS-CoV N (GST-N) protein in buffer solution could be observed from 0.1 pg/mL to 1 ng/mL. In addition, the concentration of GST-N protein in diluted serum across a similar range could also be measured. The correlation coefficients (linear scale) for these two measurements were 0.9469 and 0.9624, respectively. In comparison with conventional enzyme linked immunosorbent assay (ELISA), the detection limit of the LSPCF fiber-optic biosensor for the GST-N protein was improved at least 104-fold using the same monoclonal antibodies. Therefore, the LSPCF fiber-optic biosensor shows an ability to detect very low concentration (∼1 pg/mL) of SARS-CoV N protein in serum. The biosensor should help with the early diagnosis of SARS infection.

Original languageEnglish
Pages (from-to)320-325
Number of pages6
JournalBiosensors and Bioelectronics
Volume25
Issue number2
DOIs
Publication statusPublished - Oct 15 2009
Externally publishedYes

Fingerprint

Severe Acute Respiratory Syndrome
Biosensing Techniques
Nucleocapsid Proteins
Biosensors
Fiber optics
Fluorescence
Proteins
Serum
Immunoassay
Immunosorbents
Monoclonal antibodies
Respiratory Tract Infections
Limit of Detection
Early Diagnosis
Assays
Buffers
Enzyme-Linked Immunosorbent Assay
Monoclonal Antibodies
Technology
Enzymes

Keywords

  • Fiber-optic biosensor
  • Gold nanoparticle
  • Localized surface plasmon
  • Nucleocapsid protein
  • Severe acute respiratory syndrome (SARS)

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

Cite this

Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in human serum using a localized surface plasmon coupled fluorescence fiber-optic biosensor. / Huang, Jason C.; Chang, Ying Feng; Chen, Kuan Hsuan; Su, Li Chen; Lee, Chun Wei; Chen, Chii Chang; Chen, Yi Ming Arthur; Chou, Chien.

In: Biosensors and Bioelectronics, Vol. 25, No. 2, 15.10.2009, p. 320-325.

Research output: Contribution to journalArticle

Huang, Jason C. ; Chang, Ying Feng ; Chen, Kuan Hsuan ; Su, Li Chen ; Lee, Chun Wei ; Chen, Chii Chang ; Chen, Yi Ming Arthur ; Chou, Chien. / Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in human serum using a localized surface plasmon coupled fluorescence fiber-optic biosensor. In: Biosensors and Bioelectronics. 2009 ; Vol. 25, No. 2. pp. 320-325.
@article{db1ccddb00744ba39c37cb2564db3c5b,
title = "Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in human serum using a localized surface plasmon coupled fluorescence fiber-optic biosensor",
abstract = "In order to enhance the sensitivity of conventional immunoassay technology for the detection of SARS coronavirus (SARS-CoV) nucleocapsid protein (N protein), we developed a localized surface plasmon coupled fluorescence (LSPCF) fiber-optic biosensor that combines sandwich immunoassay with the LSP technique. Experimentally, a linear relationship between the fluorescence signal and the concentration of recombinant SARS-CoV N (GST-N) protein in buffer solution could be observed from 0.1 pg/mL to 1 ng/mL. In addition, the concentration of GST-N protein in diluted serum across a similar range could also be measured. The correlation coefficients (linear scale) for these two measurements were 0.9469 and 0.9624, respectively. In comparison with conventional enzyme linked immunosorbent assay (ELISA), the detection limit of the LSPCF fiber-optic biosensor for the GST-N protein was improved at least 104-fold using the same monoclonal antibodies. Therefore, the LSPCF fiber-optic biosensor shows an ability to detect very low concentration (∼1 pg/mL) of SARS-CoV N protein in serum. The biosensor should help with the early diagnosis of SARS infection.",
keywords = "Fiber-optic biosensor, Gold nanoparticle, Localized surface plasmon, Nucleocapsid protein, Severe acute respiratory syndrome (SARS)",
author = "Huang, {Jason C.} and Chang, {Ying Feng} and Chen, {Kuan Hsuan} and Su, {Li Chen} and Lee, {Chun Wei} and Chen, {Chii Chang} and Chen, {Yi Ming Arthur} and Chien Chou",
year = "2009",
month = "10",
day = "15",
doi = "10.1016/j.bios.2009.07.012",
language = "English",
volume = "25",
pages = "320--325",
journal = "Biosensors and Bioelectronics",
issn = "0956-5663",
publisher = "Elsevier Ltd",
number = "2",

}

TY - JOUR

T1 - Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in human serum using a localized surface plasmon coupled fluorescence fiber-optic biosensor

AU - Huang, Jason C.

AU - Chang, Ying Feng

AU - Chen, Kuan Hsuan

AU - Su, Li Chen

AU - Lee, Chun Wei

AU - Chen, Chii Chang

AU - Chen, Yi Ming Arthur

AU - Chou, Chien

PY - 2009/10/15

Y1 - 2009/10/15

N2 - In order to enhance the sensitivity of conventional immunoassay technology for the detection of SARS coronavirus (SARS-CoV) nucleocapsid protein (N protein), we developed a localized surface plasmon coupled fluorescence (LSPCF) fiber-optic biosensor that combines sandwich immunoassay with the LSP technique. Experimentally, a linear relationship between the fluorescence signal and the concentration of recombinant SARS-CoV N (GST-N) protein in buffer solution could be observed from 0.1 pg/mL to 1 ng/mL. In addition, the concentration of GST-N protein in diluted serum across a similar range could also be measured. The correlation coefficients (linear scale) for these two measurements were 0.9469 and 0.9624, respectively. In comparison with conventional enzyme linked immunosorbent assay (ELISA), the detection limit of the LSPCF fiber-optic biosensor for the GST-N protein was improved at least 104-fold using the same monoclonal antibodies. Therefore, the LSPCF fiber-optic biosensor shows an ability to detect very low concentration (∼1 pg/mL) of SARS-CoV N protein in serum. The biosensor should help with the early diagnosis of SARS infection.

AB - In order to enhance the sensitivity of conventional immunoassay technology for the detection of SARS coronavirus (SARS-CoV) nucleocapsid protein (N protein), we developed a localized surface plasmon coupled fluorescence (LSPCF) fiber-optic biosensor that combines sandwich immunoassay with the LSP technique. Experimentally, a linear relationship between the fluorescence signal and the concentration of recombinant SARS-CoV N (GST-N) protein in buffer solution could be observed from 0.1 pg/mL to 1 ng/mL. In addition, the concentration of GST-N protein in diluted serum across a similar range could also be measured. The correlation coefficients (linear scale) for these two measurements were 0.9469 and 0.9624, respectively. In comparison with conventional enzyme linked immunosorbent assay (ELISA), the detection limit of the LSPCF fiber-optic biosensor for the GST-N protein was improved at least 104-fold using the same monoclonal antibodies. Therefore, the LSPCF fiber-optic biosensor shows an ability to detect very low concentration (∼1 pg/mL) of SARS-CoV N protein in serum. The biosensor should help with the early diagnosis of SARS infection.

KW - Fiber-optic biosensor

KW - Gold nanoparticle

KW - Localized surface plasmon

KW - Nucleocapsid protein

KW - Severe acute respiratory syndrome (SARS)

UR - http://www.scopus.com/inward/record.url?scp=69949164146&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=69949164146&partnerID=8YFLogxK

U2 - 10.1016/j.bios.2009.07.012

DO - 10.1016/j.bios.2009.07.012

M3 - Article

VL - 25

SP - 320

EP - 325

JO - Biosensors and Bioelectronics

JF - Biosensors and Bioelectronics

SN - 0956-5663

IS - 2

ER -