Detection of Severe Acute Respiratory Syndrome-Associated Coronavirus in Pneumocytes of the Lung

Kuan Chih Chow, Cheng Hsiang Hsiao, Tze Yi Lin, Chi Long Chen, Shiow Her Chiou

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Previous reports have indicated that patients with severe acute respiratory syndrome (SARS)-associated coronavirus infection could develop atypical pneumonia with fulminant pulmonary edema. However, the target cells of SARS viral infection have not been characterized in detail. We report the pathologic findings of the lung in 3 cases of SARS. Chest radiographs at 2 to 3 weeks of infection revealed an atypical pneumonia with pulmonary consolidation, a clinical characteristic of SARS infection. The presence of the SARS virus was determined by nested reverse transcription-polymerase chain reaction (RT-PCR), and the infected cells were identified by in situ hybridization in open-lung biopsy and postmortem necropsy specimens. Expression of SARS virus-encoded RNA was detected in all 3 cases by RT-PCR, and the SARS viral signal was localized in pneumocytes by using in situ hybridization.

Original languageEnglish
Pages (from-to)574-580
Number of pages7
JournalAmerican Journal of Clinical Pathology
Volume121
Issue number4
DOIs
Publication statusPublished - Apr 2004
Externally publishedYes

Fingerprint

Alveolar Epithelial Cells
Severe Acute Respiratory Syndrome
Coronavirus
Lung
SARS Virus
Reverse Transcription
In Situ Hybridization
Pneumonia
Coronavirus Infections
Polymerase Chain Reaction
Virus Diseases
Pulmonary Edema
Respiratory Tract Infections
Thorax
RNA
Biopsy
Infection

Keywords

  • In situ hybridization
  • Pneumocyte
  • SARS virus
  • Surfactant

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Detection of Severe Acute Respiratory Syndrome-Associated Coronavirus in Pneumocytes of the Lung. / Chow, Kuan Chih; Hsiao, Cheng Hsiang; Lin, Tze Yi; Chen, Chi Long; Chiou, Shiow Her.

In: American Journal of Clinical Pathology, Vol. 121, No. 4, 04.2004, p. 574-580.

Research output: Contribution to journalArticle

Chow, Kuan Chih ; Hsiao, Cheng Hsiang ; Lin, Tze Yi ; Chen, Chi Long ; Chiou, Shiow Her. / Detection of Severe Acute Respiratory Syndrome-Associated Coronavirus in Pneumocytes of the Lung. In: American Journal of Clinical Pathology. 2004 ; Vol. 121, No. 4. pp. 574-580.
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