TY - JOUR
T1 - Detection of serum antibodies to three different recombinant antigens of human herpesvirus 8 by immunoblotting
T2 - Seroprevalence studies in Taiwan
AU - Wang, Yi Fen
AU - Lee, Sung Bau
AU - Cheng, Luca C.W.
AU - Tai, Ming Hong
AU - Su, Ih Jen
N1 - Funding Information:
This work was supported by National Science Council of R.O.C. Grant NSC89-2320-B-242-001. The BCBL-1 cells were provided by Dr. Jeang Kuan-Teh (NIH). We would like to thank Dr. Gary R. Whittaker for proofreading this manuscript.
PY - 2002
Y1 - 2002
N2 - Background: Human herpesvirus 8 (HHV-8), a γ-herpesvirus, is associated with Kaposi's sarcoma (KS). A variety of assays for the diagnosis HHV-8 infection have been developed. However, the accuracy of current HHV-8 antibody testing is uncertain, mainly in patients with asymptomatic HHV-8 infection. Methods: We utilized a new immunoblot assay, comprised of three recombinant HHV-8 encoded proteins (ORF16, ORF57, and ORF71) to determine the seroprevalence of HHV-8 in Taiwan. ORF16 encodes a Bcl-2 homologue and is expressed only in the lytic cycle. ORF57 is an immediate-early gene product and can activate gene expression by a post-transcriptional mechanism. ORF71 encodes a viral FLICE inhibitory protein and is a latently expressed protein. We collected 6 KS(+)/HIV(-) patients, 10 KS(-)/HIV(+) individuals, 174 blood donors, and 62 non-KS cancer patients in this study. Results: Among the different populations, ORF57 showed highest reactivities. The frequency of positive results for KS(+)/HIV(-), KS(-)/HIV(+), blood donors, and non-KS cancer patients were 83.3%, 40%, 23%, and 12.9%, respectively. Compared to the widely used lytic immunofluorescence assay, our immunoblotting assay using ORF57 showed higher reactivities among the blood donors. Conclusions: ORF57 is useful for the detection of HHV-8 infection, especially during the asyptomatic infections.
AB - Background: Human herpesvirus 8 (HHV-8), a γ-herpesvirus, is associated with Kaposi's sarcoma (KS). A variety of assays for the diagnosis HHV-8 infection have been developed. However, the accuracy of current HHV-8 antibody testing is uncertain, mainly in patients with asymptomatic HHV-8 infection. Methods: We utilized a new immunoblot assay, comprised of three recombinant HHV-8 encoded proteins (ORF16, ORF57, and ORF71) to determine the seroprevalence of HHV-8 in Taiwan. ORF16 encodes a Bcl-2 homologue and is expressed only in the lytic cycle. ORF57 is an immediate-early gene product and can activate gene expression by a post-transcriptional mechanism. ORF71 encodes a viral FLICE inhibitory protein and is a latently expressed protein. We collected 6 KS(+)/HIV(-) patients, 10 KS(-)/HIV(+) individuals, 174 blood donors, and 62 non-KS cancer patients in this study. Results: Among the different populations, ORF57 showed highest reactivities. The frequency of positive results for KS(+)/HIV(-), KS(-)/HIV(+), blood donors, and non-KS cancer patients were 83.3%, 40%, 23%, and 12.9%, respectively. Compared to the widely used lytic immunofluorescence assay, our immunoblotting assay using ORF57 showed higher reactivities among the blood donors. Conclusions: ORF57 is useful for the detection of HHV-8 infection, especially during the asyptomatic infections.
KW - Human herpesvirus 8
KW - Immunoblotting
KW - Seroprevalence
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U2 - 10.1016/S0009-8981(02)00023-2
DO - 10.1016/S0009-8981(02)00023-2
M3 - Article
C2 - 11983198
AN - SCOPUS:0036238424
SN - 0009-8981
VL - 320
SP - 37
EP - 42
JO - Clinica Chimica Acta
JF - Clinica Chimica Acta
IS - 1-2
ER -
