Detection of mycobacterial infection in paraffin-embedded pathologic tissues by DNA polymerase chain reaction: Comparison with conventional histochemical stain

I. Ping Chiang, Lai Fong Kok, Chi Long Chen

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Abstract

Objectives. Tuberculosis caused by Mycobacterium tuberculosis is one of the most common infectious diseases worldwide. Pathologic confirmation by demonstrating mycobacterial bacillus in tissue section using histochemical stain or culture is the traditional diagnostic method. However, the diagnostic rate by both histochemical stain and culture is low. Polymerase chain reaction (PCR) is able to detect trace amounts of nucleic acid in paraffin sections. We detected mycobacterial DNA in pathological samples by PCR and compared the results with those of histochemical stain and culture. Methods. Formalin-fixed paraffin-embedded tissue sections of 16 acid-fast positive and 17 acid-fast negative tissue samples showing granulomatous inflammation were retrieved retrospectively for PCR study. Results. PCR detected mycobacterial DNA in 14 out of 16 acid-fast positive and 14 out of 17 acid-fast negative samples. The sensitivity and specificity for detecting Mycobacterium by PCR were much higher than acid-fast stain and culture. Conclusions. PCR method was very useful in detecting mycobacterial infection in pathologic sections. We believe that the establishment of the PCR method in the department of pathology will improve the quality of clinical and pathologic diagnosis of tuberculosis.

Original languageEnglish
Pages (from-to)25-31
Number of pages7
JournalMid-Taiwan Journal of Medicine
Volume10
Issue number1
Publication statusPublished - Mar 2005

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DNA-Directed DNA Polymerase
Paraffin
Coloring Agents
Polymerase Chain Reaction
Infection
Acids
Tuberculosis
DNA
Mycobacterium
Mycobacterium tuberculosis
Nucleic Acids
Bacillus
Formaldehyde
Communicable Diseases
Pathology
Inflammation
Sensitivity and Specificity

Keywords

  • Mycobacterium
  • PCR
  • Tuberculosis

ASJC Scopus subject areas

  • Medicine(all)

Cite this

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title = "Detection of mycobacterial infection in paraffin-embedded pathologic tissues by DNA polymerase chain reaction: Comparison with conventional histochemical stain",
abstract = "Objectives. Tuberculosis caused by Mycobacterium tuberculosis is one of the most common infectious diseases worldwide. Pathologic confirmation by demonstrating mycobacterial bacillus in tissue section using histochemical stain or culture is the traditional diagnostic method. However, the diagnostic rate by both histochemical stain and culture is low. Polymerase chain reaction (PCR) is able to detect trace amounts of nucleic acid in paraffin sections. We detected mycobacterial DNA in pathological samples by PCR and compared the results with those of histochemical stain and culture. Methods. Formalin-fixed paraffin-embedded tissue sections of 16 acid-fast positive and 17 acid-fast negative tissue samples showing granulomatous inflammation were retrieved retrospectively for PCR study. Results. PCR detected mycobacterial DNA in 14 out of 16 acid-fast positive and 14 out of 17 acid-fast negative samples. The sensitivity and specificity for detecting Mycobacterium by PCR were much higher than acid-fast stain and culture. Conclusions. PCR method was very useful in detecting mycobacterial infection in pathologic sections. We believe that the establishment of the PCR method in the department of pathology will improve the quality of clinical and pathologic diagnosis of tuberculosis.",
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AU - Chiang, I. Ping

AU - Kok, Lai Fong

AU - Chen, Chi Long

PY - 2005/3

Y1 - 2005/3

N2 - Objectives. Tuberculosis caused by Mycobacterium tuberculosis is one of the most common infectious diseases worldwide. Pathologic confirmation by demonstrating mycobacterial bacillus in tissue section using histochemical stain or culture is the traditional diagnostic method. However, the diagnostic rate by both histochemical stain and culture is low. Polymerase chain reaction (PCR) is able to detect trace amounts of nucleic acid in paraffin sections. We detected mycobacterial DNA in pathological samples by PCR and compared the results with those of histochemical stain and culture. Methods. Formalin-fixed paraffin-embedded tissue sections of 16 acid-fast positive and 17 acid-fast negative tissue samples showing granulomatous inflammation were retrieved retrospectively for PCR study. Results. PCR detected mycobacterial DNA in 14 out of 16 acid-fast positive and 14 out of 17 acid-fast negative samples. The sensitivity and specificity for detecting Mycobacterium by PCR were much higher than acid-fast stain and culture. Conclusions. PCR method was very useful in detecting mycobacterial infection in pathologic sections. We believe that the establishment of the PCR method in the department of pathology will improve the quality of clinical and pathologic diagnosis of tuberculosis.

AB - Objectives. Tuberculosis caused by Mycobacterium tuberculosis is one of the most common infectious diseases worldwide. Pathologic confirmation by demonstrating mycobacterial bacillus in tissue section using histochemical stain or culture is the traditional diagnostic method. However, the diagnostic rate by both histochemical stain and culture is low. Polymerase chain reaction (PCR) is able to detect trace amounts of nucleic acid in paraffin sections. We detected mycobacterial DNA in pathological samples by PCR and compared the results with those of histochemical stain and culture. Methods. Formalin-fixed paraffin-embedded tissue sections of 16 acid-fast positive and 17 acid-fast negative tissue samples showing granulomatous inflammation were retrieved retrospectively for PCR study. Results. PCR detected mycobacterial DNA in 14 out of 16 acid-fast positive and 14 out of 17 acid-fast negative samples. The sensitivity and specificity for detecting Mycobacterium by PCR were much higher than acid-fast stain and culture. Conclusions. PCR method was very useful in detecting mycobacterial infection in pathologic sections. We believe that the establishment of the PCR method in the department of pathology will improve the quality of clinical and pathologic diagnosis of tuberculosis.

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