Detection of lower levels of SNAP25 using multiple microarray systems and its functional significance in medulloblastoma

Chi Jung Huang, Chia Long Lee, Chih Yi Liu, Shih Hung Huang, Jia Woei Hou, Yi Hou Chen, Chih Cheng Chien, Chih Ming Ho, Wen Cheng Lo, Kun Long Hung

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Medulloblastoma (MB) is the most common pediatric malignant brain tumor and patients with high-risk or recurrent MB respond poorly to current therapies, and have a higher related mortality. For this reason, potential molecules related to MB need be identified in order to develop targets for the development of novel therapeutics. In the present study, we compared MB microarray data obtained using different microarray systems and significant targets were selected by gene annotation and enrichment analysis. Genes for soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) annotated with the function 'vesicle' were identified and one of these proteins, synaptosomal-associated protein 25 (SNAP25), was found to have significantly lower expression levels in MB. In addition, SNAP25 was detected in a very low number of MB cells as shown by western blot analysis and immunohistochemical analyses of archived and formalin-fixed/paraffin-embedded human MB specimens. We found that SNAP25 altered the morphology and the chemotherapeutic effects of arabinofuranosyl cytidine (Ara-C) on SNAP25-expressing MB cells. On the whole, our data indicate that the expression of SNAP25 is crucial for dendrite formation and is associated with the effects of targeted chemotherapy. The detection of SNAP25 expression in MB cells may thus be essential for the chemotherapeutic application of Ara-C.

Original languageEnglish
Pages (from-to)1195-1205
Number of pages11
JournalInternational Journal of Molecular Medicine
Volume39
Issue number5
DOIs
Publication statusPublished - May 1 2017

Keywords

  • Arabinofuranosyl cytidine
  • Dendrite density
  • Gene Expression Omnibus
  • Medulloblastoma
  • Synaptosomal-associated protein 25

ASJC Scopus subject areas

  • Genetics

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