Detection of K-ras point mutations in codons 12 and 13 in non-small cell lung cancers

Nan Yung Hsu, Chih Yi Chen, Chii Huei Wu, Tse Fia Liu, Po Cheung Kwam, Chung Ping Hsu, Jiun Yi Hsia, Wu Tsung Chang

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3 Citations (Scopus)

Abstract

Point mutations of the K-ras gene have been reported in a wide variety of human tumors. By using polymerase chain reaction followed by direct DNA sequencing, we screened for point mutations at codons 12 and 13 of the K-ras gene in specimens obtained from fresh frozen tumors in 38 patients with non-small cell lung cancers. Point mutations were detected in two of 38 (5.3%) resected non-small cell lung cancers. Both of them were G to T transversions. One patient was found to have a K-ras codon 13 point mutation (GGC to TGC, gly to cys), while the other had a codon 12 point mutation (GGT to GTT, gly to val). Based on the limited numbers in this study, we found that the frequency of K-ras point mutations in codons 12 and 13 among Asian patients with lung adenocarcinomas was lower than that detected among Caucasian patients.

Original languageEnglish
Pages (from-to)741-745
Number of pages5
JournalJournal of the Formosan Medical Association = Taiwan yi zhi
Volume95
Issue number10
Publication statusPublished - 1996
Externally publishedYes

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Keywords

  • codon 12
  • codon 13
  • K-ras gene
  • mutation
  • non-small cell lung cancer

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Hsu, N. Y., Chen, C. Y., Wu, C. H., Liu, T. F., Kwam, P. C., Hsu, C. P., Hsia, J. Y., & Chang, W. T. (1996). Detection of K-ras point mutations in codons 12 and 13 in non-small cell lung cancers. Journal of the Formosan Medical Association = Taiwan yi zhi, 95(10), 741-745.