Detection of K-ras point mutations in codons 12 and 13 in non-small cell lung cancers

Nan Yung Hsu, Chih Yi Chen, Chii Huei Wu, Tse Fia Liu, Po Cheung Kwam, Chung Ping Hsu, Jiun Yi Hsia, Wu Tsung Chang

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Point mutations of the K-ras gene have been reported in a wide variety of human tumors. By using polymerase chain reaction followed by direct DNA sequencing, we screened for point mutations at codons 12 and 13 of the K-ras gene in specimens obtained from fresh frozen tumors in 38 patients with non-small cell lung cancers. Point mutations were detected in two of 38 (5.3%) resected non-small cell lung cancers. Both of them were G to T transversions. One patient was found to have a K-ras codon 13 point mutation (GGC to TGC, gly to cys), while the other had a codon 12 point mutation (GGT to GTT, gly to val). Based on the limited numbers in this study, we found that the frequency of K-ras point mutations in codons 12 and 13 among Asian patients with lung adenocarcinomas was lower than that detected among Caucasian patients.

Original languageEnglish
Pages (from-to)741-745
Number of pages5
JournalJournal of the Formosan Medical Association = Taiwan yi zhi
Volume95
Issue number10
Publication statusPublished - 1996
Externally publishedYes

Fingerprint

Point Mutation
Non-Small Cell Lung Carcinoma
Codon
ras Genes
DNA Sequence Analysis
Neoplasms
Polymerase Chain Reaction

Keywords

  • codon 12
  • codon 13
  • K-ras gene
  • mutation
  • non-small cell lung cancer

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Hsu, N. Y., Chen, C. Y., Wu, C. H., Liu, T. F., Kwam, P. C., Hsu, C. P., ... Chang, W. T. (1996). Detection of K-ras point mutations in codons 12 and 13 in non-small cell lung cancers. Journal of the Formosan Medical Association = Taiwan yi zhi, 95(10), 741-745.

Detection of K-ras point mutations in codons 12 and 13 in non-small cell lung cancers. / Hsu, Nan Yung; Chen, Chih Yi; Wu, Chii Huei; Liu, Tse Fia; Kwam, Po Cheung; Hsu, Chung Ping; Hsia, Jiun Yi; Chang, Wu Tsung.

In: Journal of the Formosan Medical Association = Taiwan yi zhi, Vol. 95, No. 10, 1996, p. 741-745.

Research output: Contribution to journalArticle

Hsu, NY, Chen, CY, Wu, CH, Liu, TF, Kwam, PC, Hsu, CP, Hsia, JY & Chang, WT 1996, 'Detection of K-ras point mutations in codons 12 and 13 in non-small cell lung cancers', Journal of the Formosan Medical Association = Taiwan yi zhi, vol. 95, no. 10, pp. 741-745.
Hsu, Nan Yung ; Chen, Chih Yi ; Wu, Chii Huei ; Liu, Tse Fia ; Kwam, Po Cheung ; Hsu, Chung Ping ; Hsia, Jiun Yi ; Chang, Wu Tsung. / Detection of K-ras point mutations in codons 12 and 13 in non-small cell lung cancers. In: Journal of the Formosan Medical Association = Taiwan yi zhi. 1996 ; Vol. 95, No. 10. pp. 741-745.
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AU - Kwam, Po Cheung

AU - Hsu, Chung Ping

AU - Hsia, Jiun Yi

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AB - Point mutations of the K-ras gene have been reported in a wide variety of human tumors. By using polymerase chain reaction followed by direct DNA sequencing, we screened for point mutations at codons 12 and 13 of the K-ras gene in specimens obtained from fresh frozen tumors in 38 patients with non-small cell lung cancers. Point mutations were detected in two of 38 (5.3%) resected non-small cell lung cancers. Both of them were G to T transversions. One patient was found to have a K-ras codon 13 point mutation (GGC to TGC, gly to cys), while the other had a codon 12 point mutation (GGT to GTT, gly to val). Based on the limited numbers in this study, we found that the frequency of K-ras point mutations in codons 12 and 13 among Asian patients with lung adenocarcinomas was lower than that detected among Caucasian patients.

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