Detection of human cytomegalovirus retinitis and monitoring of ganciclovir treatment using conjunctival swab with polymerase chain reaction in AIDS patients

S. H. Chiou, J. H. Liu, W. W. Wong, Y. J. Chan, Y. C. Chang, J. J. Wang, C. Y. Liu, W. T. Liu, S. C. Chen, Wen-Ming Hsu

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

This report studies the accuracy of conjunctival swab polymerase chain reaction (CS-PCR) for the diagnosis of human cytomegalovirus retinitis (HCMV) in AIDS patients. PCR and virus culture were used for the detection of HCMV in conjunctival swab, serum, and urine specimens from 38 AIDS patients between April 1996 and April 1998. The clinical utility of the identification of HCMV retinitis by these 6 different methods was demonstrated by their prediction power to estimate AIDS patients at risk of contracting HCMV retinitis. The sensitivity, specificity, positive predictive value, and negative predictive value of CS-PCR for the detection of HCMV retinitis were 91.5%, 80.9%, 60.8%, and 92.7%, respectively; for serum PCR were 74.3%, 81.7%, 57.2%, and 90.3%; for urine PCR were 100%, 17.3%, 20.4%, and 100%; for conjunctival swab culture were 22.7%, 100%, 100%, and 86%; for serum culture were 27.3%, 98.1%, 75%, and 86.4%; and for urine culture were 90.9%, 44.2%, 25.6%, and 95.8%.

Original languageEnglish
Pages (from-to)85-91
Number of pages7
JournalInternational Journal of STD and AIDS
Volume11
Issue number2
DOIs
Publication statusPublished - Feb 16 2000
Externally publishedYes

Keywords

  • AIDS
  • Conjunctival swab
  • Human cytomegalovirus retinitis
  • Polymerase chain reaction

ASJC Scopus subject areas

  • Dermatology
  • Public Health, Environmental and Occupational Health
  • Pharmacology (medical)
  • Infectious Diseases

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