Detection of Epstein-Barr virus in esophageal squamous cell carcinoma in Taiwan

Liang Shun Wang, Kuan Chih Chow, Yu Chung Wu, Wing Y. Li, Min Hsiung Huang

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

OBJECTIVE: Recently, an association between viral infection and the development of esophageal carcinoma has been reported, particularly the human papilloma virus (HPV) and Epstein-Barr virus (EBV). However, geographic variation in carcinogenesis is realized. In this study, we investigate the viral carcinogenesis and the biologic effect of viral infection on esophageal squamous cell carcinoma (ESCC) in Taiwan. METHODS: To determine the association of viral infection (EBV and HPV) with ESCC, we applied polymerase chain reaction (PCR), immunohistochemistry, and in situ hybridization (ISH) to examine 119 surgical specimens from different sites of esophagus in 31 ESCC patients. Additionally, an immunoperoxidase method was used to detect EBV latent membrane protein-1 (LMP-1), p53, CD45RO (UCHL-1), Fas ligand (Fas L), and RNA ISH with oligonucleotide sequences was used to detected interleukin-6 (IL-6) mRNA. RESULTS: By PCR, EBV DNA was detected in 11 cases (35.5%). Expression of EBERs in ESCC was further confirmed with ISH. Nonetheless, no LMP-1 expression was detected. On the other hand, human papillomavirus (HPV) was identified in only one case (3.2%) of ESCC. Furthermore, HPV was located by ISH in the distant normal region rather than in tumor cells. In EBV-positive cases, accumulation of p53 protein was detected in 10 lesions (91%); CD45RO+ lymphocytes together with expressions of FasL and IL-6 were respectively identified in 100%, 63.6%, and 54.5% of 11 EBV-positive lesions. Interestingly, in the EBV-negative cases (n = 20), p53 protein was detected in 40% of lesions; CD45RO 30%; FasL 50%, and IL-6 10%. CONCLUSIONS: In this study, no correlation was found between the presence of EBV in ESCC and the patients' age, sex, as well as survival. Although our results indicate that EBV could be associated with ESCC, the clinical role of EBV in ESCC remains to be determined.

Original languageEnglish
Pages (from-to)2834-2839
Number of pages6
JournalAmerican Journal of Gastroenterology
Volume94
Issue number10
DOIs
Publication statusPublished - Oct 1999
Externally publishedYes

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Human Herpesvirus 4
Taiwan
Papillomaviridae
In Situ Hybridization
Virus Diseases
Interleukin-6
Carcinogenesis
Esophageal Squamous Cell Carcinoma
Polymerase Chain Reaction
Fas Ligand Protein
Oligonucleotides
Interleukin-10
Esophagus
Membrane Proteins
Proteins
Immunohistochemistry
Lymphocytes
RNA
Carcinoma
Messenger RNA

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Detection of Epstein-Barr virus in esophageal squamous cell carcinoma in Taiwan. / Wang, Liang Shun; Chow, Kuan Chih; Wu, Yu Chung; Li, Wing Y.; Huang, Min Hsiung.

In: American Journal of Gastroenterology, Vol. 94, No. 10, 10.1999, p. 2834-2839.

Research output: Contribution to journalArticle

Wang, Liang Shun ; Chow, Kuan Chih ; Wu, Yu Chung ; Li, Wing Y. ; Huang, Min Hsiung. / Detection of Epstein-Barr virus in esophageal squamous cell carcinoma in Taiwan. In: American Journal of Gastroenterology. 1999 ; Vol. 94, No. 10. pp. 2834-2839.
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abstract = "OBJECTIVE: Recently, an association between viral infection and the development of esophageal carcinoma has been reported, particularly the human papilloma virus (HPV) and Epstein-Barr virus (EBV). However, geographic variation in carcinogenesis is realized. In this study, we investigate the viral carcinogenesis and the biologic effect of viral infection on esophageal squamous cell carcinoma (ESCC) in Taiwan. METHODS: To determine the association of viral infection (EBV and HPV) with ESCC, we applied polymerase chain reaction (PCR), immunohistochemistry, and in situ hybridization (ISH) to examine 119 surgical specimens from different sites of esophagus in 31 ESCC patients. Additionally, an immunoperoxidase method was used to detect EBV latent membrane protein-1 (LMP-1), p53, CD45RO (UCHL-1), Fas ligand (Fas L), and RNA ISH with oligonucleotide sequences was used to detected interleukin-6 (IL-6) mRNA. RESULTS: By PCR, EBV DNA was detected in 11 cases (35.5{\%}). Expression of EBERs in ESCC was further confirmed with ISH. Nonetheless, no LMP-1 expression was detected. On the other hand, human papillomavirus (HPV) was identified in only one case (3.2{\%}) of ESCC. Furthermore, HPV was located by ISH in the distant normal region rather than in tumor cells. In EBV-positive cases, accumulation of p53 protein was detected in 10 lesions (91{\%}); CD45RO+ lymphocytes together with expressions of FasL and IL-6 were respectively identified in 100{\%}, 63.6{\%}, and 54.5{\%} of 11 EBV-positive lesions. Interestingly, in the EBV-negative cases (n = 20), p53 protein was detected in 40{\%} of lesions; CD45RO 30{\%}; FasL 50{\%}, and IL-6 10{\%}. CONCLUSIONS: In this study, no correlation was found between the presence of EBV in ESCC and the patients' age, sex, as well as survival. Although our results indicate that EBV could be associated with ESCC, the clinical role of EBV in ESCC remains to be determined.",
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