Detection of Epstein-Barr virus genome within thymic epithelial tumours in Taiwanese patients by nested PCR, PCR in situ hybridization, and RNA in situ hybridization

P. C H Chen, Chin Chen Pan, An Hang Yang, Liang Shun Wang, Hung Chiang

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Epstein-Barr virus (EBV) is known to be associated with a variety of tumours, including Burkitt's lymphoma, nasopharyngeal carcinoma, and some carcinomas of other organs with similar lymphoepithelioma-like features. The association between EBV and thymic epithelial tumours is inconclusive, as reports in this regard are not entirely consistent and the methods employed are of different sensitivity and specificity. This study examined 78 thymomas and 21 thymic carcinomas in Taiwanese patients, to detect the viral genome at both DNA and RNA levels. The tissue blocks were first screened by nested polymerase chain reaction (PCR) targeting on the first tandem internal repeats. The positive cases were further submitted for viral localization by in situ PCR in situ hybridization (ISH) and Epstein-Barr-encoded RNA-1 (EBER-1) ISH. None of the thymomas showed a detectable EBV genome. Eight thymic carcinomas were positive for EBV by nested PCR, of which six displayed signals within the tumour cells by in situ PCR ISH and/or RNA ISH, one displayed signals within the lymphocytes, and one showed no discernible in situ signals. Most of them exhibited a lymphoepithelioma-like morphology. These results show that nested PCR is a sensitive method for screening the EBV genome in thymic epithelial tumours. In situ PCR ISH is reliable for localization of the virus, in addition to EBER-1 RNA ISH. Thymomas are not related to EBV, even in this endemic area. Thymic carcinomas, especially the lymphoepithelioma-like thymic carcinomas, are more often associated with the virus.

Original languageEnglish
Pages (from-to)684-688
Number of pages5
JournalJournal of Pathology
Volume197
Issue number5
DOIs
Publication statusPublished - 2002
Externally publishedYes

Fingerprint

Thymoma
Human Herpesvirus 4
In Situ Hybridization
Genome
RNA
Polymerase Chain Reaction
Satellite Viruses
Tandem Repeat Sequences
Burkitt Lymphoma
Viral Genome
Thymic epithelial tumor
Neoplasms
Lymphocytes
Viruses
Carcinoma
Sensitivity and Specificity
DNA

Keywords

  • EBER-1 RNA
  • Epstein-Barr virus (EBV)
  • In situ hybridization
  • Lymphoepithelioma-like thymic carcinoma
  • Nested polymerase chain reaction situ PCR
  • Thymic carcinoma
  • Thymoma

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Detection of Epstein-Barr virus genome within thymic epithelial tumours in Taiwanese patients by nested PCR, PCR in situ hybridization, and RNA in situ hybridization. / Chen, P. C H; Pan, Chin Chen; Yang, An Hang; Wang, Liang Shun; Chiang, Hung.

In: Journal of Pathology, Vol. 197, No. 5, 2002, p. 684-688.

Research output: Contribution to journalArticle

Chen, P. C H ; Pan, Chin Chen ; Yang, An Hang ; Wang, Liang Shun ; Chiang, Hung. / Detection of Epstein-Barr virus genome within thymic epithelial tumours in Taiwanese patients by nested PCR, PCR in situ hybridization, and RNA in situ hybridization. In: Journal of Pathology. 2002 ; Vol. 197, No. 5. pp. 684-688.
@article{60066628bd19426ca62cc068d7ee1e85,
title = "Detection of Epstein-Barr virus genome within thymic epithelial tumours in Taiwanese patients by nested PCR, PCR in situ hybridization, and RNA in situ hybridization",
abstract = "Epstein-Barr virus (EBV) is known to be associated with a variety of tumours, including Burkitt's lymphoma, nasopharyngeal carcinoma, and some carcinomas of other organs with similar lymphoepithelioma-like features. The association between EBV and thymic epithelial tumours is inconclusive, as reports in this regard are not entirely consistent and the methods employed are of different sensitivity and specificity. This study examined 78 thymomas and 21 thymic carcinomas in Taiwanese patients, to detect the viral genome at both DNA and RNA levels. The tissue blocks were first screened by nested polymerase chain reaction (PCR) targeting on the first tandem internal repeats. The positive cases were further submitted for viral localization by in situ PCR in situ hybridization (ISH) and Epstein-Barr-encoded RNA-1 (EBER-1) ISH. None of the thymomas showed a detectable EBV genome. Eight thymic carcinomas were positive for EBV by nested PCR, of which six displayed signals within the tumour cells by in situ PCR ISH and/or RNA ISH, one displayed signals within the lymphocytes, and one showed no discernible in situ signals. Most of them exhibited a lymphoepithelioma-like morphology. These results show that nested PCR is a sensitive method for screening the EBV genome in thymic epithelial tumours. In situ PCR ISH is reliable for localization of the virus, in addition to EBER-1 RNA ISH. Thymomas are not related to EBV, even in this endemic area. Thymic carcinomas, especially the lymphoepithelioma-like thymic carcinomas, are more often associated with the virus.",
keywords = "EBER-1 RNA, Epstein-Barr virus (EBV), In situ hybridization, Lymphoepithelioma-like thymic carcinoma, Nested polymerase chain reaction situ PCR, Thymic carcinoma, Thymoma",
author = "Chen, {P. C H} and Pan, {Chin Chen} and Yang, {An Hang} and Wang, {Liang Shun} and Hung Chiang",
year = "2002",
doi = "10.1002/path.1141",
language = "English",
volume = "197",
pages = "684--688",
journal = "Journal of Pathology",
issn = "0022-3417",
publisher = "John Wiley and Sons Ltd",
number = "5",

}

TY - JOUR

T1 - Detection of Epstein-Barr virus genome within thymic epithelial tumours in Taiwanese patients by nested PCR, PCR in situ hybridization, and RNA in situ hybridization

AU - Chen, P. C H

AU - Pan, Chin Chen

AU - Yang, An Hang

AU - Wang, Liang Shun

AU - Chiang, Hung

PY - 2002

Y1 - 2002

N2 - Epstein-Barr virus (EBV) is known to be associated with a variety of tumours, including Burkitt's lymphoma, nasopharyngeal carcinoma, and some carcinomas of other organs with similar lymphoepithelioma-like features. The association between EBV and thymic epithelial tumours is inconclusive, as reports in this regard are not entirely consistent and the methods employed are of different sensitivity and specificity. This study examined 78 thymomas and 21 thymic carcinomas in Taiwanese patients, to detect the viral genome at both DNA and RNA levels. The tissue blocks were first screened by nested polymerase chain reaction (PCR) targeting on the first tandem internal repeats. The positive cases were further submitted for viral localization by in situ PCR in situ hybridization (ISH) and Epstein-Barr-encoded RNA-1 (EBER-1) ISH. None of the thymomas showed a detectable EBV genome. Eight thymic carcinomas were positive for EBV by nested PCR, of which six displayed signals within the tumour cells by in situ PCR ISH and/or RNA ISH, one displayed signals within the lymphocytes, and one showed no discernible in situ signals. Most of them exhibited a lymphoepithelioma-like morphology. These results show that nested PCR is a sensitive method for screening the EBV genome in thymic epithelial tumours. In situ PCR ISH is reliable for localization of the virus, in addition to EBER-1 RNA ISH. Thymomas are not related to EBV, even in this endemic area. Thymic carcinomas, especially the lymphoepithelioma-like thymic carcinomas, are more often associated with the virus.

AB - Epstein-Barr virus (EBV) is known to be associated with a variety of tumours, including Burkitt's lymphoma, nasopharyngeal carcinoma, and some carcinomas of other organs with similar lymphoepithelioma-like features. The association between EBV and thymic epithelial tumours is inconclusive, as reports in this regard are not entirely consistent and the methods employed are of different sensitivity and specificity. This study examined 78 thymomas and 21 thymic carcinomas in Taiwanese patients, to detect the viral genome at both DNA and RNA levels. The tissue blocks were first screened by nested polymerase chain reaction (PCR) targeting on the first tandem internal repeats. The positive cases were further submitted for viral localization by in situ PCR in situ hybridization (ISH) and Epstein-Barr-encoded RNA-1 (EBER-1) ISH. None of the thymomas showed a detectable EBV genome. Eight thymic carcinomas were positive for EBV by nested PCR, of which six displayed signals within the tumour cells by in situ PCR ISH and/or RNA ISH, one displayed signals within the lymphocytes, and one showed no discernible in situ signals. Most of them exhibited a lymphoepithelioma-like morphology. These results show that nested PCR is a sensitive method for screening the EBV genome in thymic epithelial tumours. In situ PCR ISH is reliable for localization of the virus, in addition to EBER-1 RNA ISH. Thymomas are not related to EBV, even in this endemic area. Thymic carcinomas, especially the lymphoepithelioma-like thymic carcinomas, are more often associated with the virus.

KW - EBER-1 RNA

KW - Epstein-Barr virus (EBV)

KW - In situ hybridization

KW - Lymphoepithelioma-like thymic carcinoma

KW - Nested polymerase chain reaction situ PCR

KW - Thymic carcinoma

KW - Thymoma

UR - http://www.scopus.com/inward/record.url?scp=0036059692&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036059692&partnerID=8YFLogxK

U2 - 10.1002/path.1141

DO - 10.1002/path.1141

M3 - Article

VL - 197

SP - 684

EP - 688

JO - Journal of Pathology

JF - Journal of Pathology

SN - 0022-3417

IS - 5

ER -