Detection of cefotaxime-resistant CTX-M-3 in clinical isolates of Serratia marcescens

Mei Fen Tsou, Wen Liang Yu, Wan Hung Siu, Chizh Ju Chi, [No Value] Chieh-Ju, Ching Chen Ssu, Lii Tzu Wu

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Strains of Serratia marcescens (isolated in a hospital during April and August 2000) resistant to ampicillin, chloramphenicol, trimethoprim- sulfamethoxazole, streptomycin, tetracycline, and gentamicin were characterized. Out of a total of 34 clinical isolates 6 (17.6 %) exhibited the extended spectrum β-lactamases (ESBL) resistance; they were also resistant to cefotaxime (minimum inhibitory concentration, MIC ≥ 128 μg/mL) but susceptible to imipenem (MIC ≤ 0.5 μg/mL). This multidrug resistance was shown to be transferred by a conjugative plasmid. Transconjugants revealed similar MIC profiles when compared to the parental strains. Isoelectric focusing revealed one major transferable β-lactamase (pI 8.4) which was further identified as CTX-M-3 by PCR and gene sequencing. The presence of strains with this type of ESBL showed the evolution of bla genes and their dissemination among at least three species of the family Enterobacteriaceae isolated within a single hospital. The predominance of CTX-M type enzymes found in this area of Taiwan appeared to be similar to that described in Poland.

Original languageEnglish
Pages (from-to)452-456
Number of pages5
JournalFolia Microbiologica
Volume49
Issue number4
Publication statusPublished - 2004
Externally publishedYes

Fingerprint

Serratia marcescens
Cefotaxime
Imipenem
Sulfamethoxazole Drug Combination Trimethoprim
Isoelectric Focusing
Microbial Sensitivity Tests
Multiple Drug Resistance
Streptomycin
Enterobacteriaceae
Chloramphenicol
Poland
Ampicillin
Gentamicins
Tetracycline
Taiwan
Genes
Plasmids
Polymerase Chain Reaction
Enzymes

ASJC Scopus subject areas

  • Microbiology

Cite this

Tsou, M. F., Yu, W. L., Siu, W. H., Chi, C. J., Chieh-Ju, N. V., Ssu, C. C., & Wu, L. T. (2004). Detection of cefotaxime-resistant CTX-M-3 in clinical isolates of Serratia marcescens. Folia Microbiologica, 49(4), 452-456.

Detection of cefotaxime-resistant CTX-M-3 in clinical isolates of Serratia marcescens. / Tsou, Mei Fen; Yu, Wen Liang; Siu, Wan Hung; Chi, Chizh Ju; Chieh-Ju, [No Value]; Ssu, Ching Chen; Wu, Lii Tzu.

In: Folia Microbiologica, Vol. 49, No. 4, 2004, p. 452-456.

Research output: Contribution to journalArticle

Tsou, MF, Yu, WL, Siu, WH, Chi, CJ, Chieh-Ju, NV, Ssu, CC & Wu, LT 2004, 'Detection of cefotaxime-resistant CTX-M-3 in clinical isolates of Serratia marcescens', Folia Microbiologica, vol. 49, no. 4, pp. 452-456.
Tsou MF, Yu WL, Siu WH, Chi CJ, Chieh-Ju NV, Ssu CC et al. Detection of cefotaxime-resistant CTX-M-3 in clinical isolates of Serratia marcescens. Folia Microbiologica. 2004;49(4):452-456.
Tsou, Mei Fen ; Yu, Wen Liang ; Siu, Wan Hung ; Chi, Chizh Ju ; Chieh-Ju, [No Value] ; Ssu, Ching Chen ; Wu, Lii Tzu. / Detection of cefotaxime-resistant CTX-M-3 in clinical isolates of Serratia marcescens. In: Folia Microbiologica. 2004 ; Vol. 49, No. 4. pp. 452-456.
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