TY - JOUR
T1 - Detection of cefotaxime-resistant CTX-M-3 in clinical isolates of Serratia marcescens
AU - Tsou, Mei Fen
AU - Yu, Wen Liang
AU - Siu, Wan Hung
AU - Chi, Chizh Ju
AU - Chieh-Ju, [No Value]
AU - Ssu, Ching Chen
AU - Wu, Lii Tzu
PY - 2004
Y1 - 2004
N2 - Strains of Serratia marcescens (isolated in a hospital during April and August 2000) resistant to ampicillin, chloramphenicol, trimethoprim- sulfamethoxazole, streptomycin, tetracycline, and gentamicin were characterized. Out of a total of 34 clinical isolates 6 (17.6 %) exhibited the extended spectrum β-lactamases (ESBL) resistance; they were also resistant to cefotaxime (minimum inhibitory concentration, MIC ≥ 128 μg/mL) but susceptible to imipenem (MIC ≤ 0.5 μg/mL). This multidrug resistance was shown to be transferred by a conjugative plasmid. Transconjugants revealed similar MIC profiles when compared to the parental strains. Isoelectric focusing revealed one major transferable β-lactamase (pI 8.4) which was further identified as CTX-M-3 by PCR and gene sequencing. The presence of strains with this type of ESBL showed the evolution of bla genes and their dissemination among at least three species of the family Enterobacteriaceae isolated within a single hospital. The predominance of CTX-M type enzymes found in this area of Taiwan appeared to be similar to that described in Poland.
AB - Strains of Serratia marcescens (isolated in a hospital during April and August 2000) resistant to ampicillin, chloramphenicol, trimethoprim- sulfamethoxazole, streptomycin, tetracycline, and gentamicin were characterized. Out of a total of 34 clinical isolates 6 (17.6 %) exhibited the extended spectrum β-lactamases (ESBL) resistance; they were also resistant to cefotaxime (minimum inhibitory concentration, MIC ≥ 128 μg/mL) but susceptible to imipenem (MIC ≤ 0.5 μg/mL). This multidrug resistance was shown to be transferred by a conjugative plasmid. Transconjugants revealed similar MIC profiles when compared to the parental strains. Isoelectric focusing revealed one major transferable β-lactamase (pI 8.4) which was further identified as CTX-M-3 by PCR and gene sequencing. The presence of strains with this type of ESBL showed the evolution of bla genes and their dissemination among at least three species of the family Enterobacteriaceae isolated within a single hospital. The predominance of CTX-M type enzymes found in this area of Taiwan appeared to be similar to that described in Poland.
UR - http://www.scopus.com/inward/record.url?scp=5444275961&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=5444275961&partnerID=8YFLogxK
M3 - Article
C2 - 15530012
AN - SCOPUS:5444275961
VL - 49
SP - 452
EP - 456
JO - Folia Microbiologica
JF - Folia Microbiologica
SN - 0015-5632
IS - 4
ER -