Detection and identification of human parechoviruses from clinical specimens

Bao Chen Chen, Ming Fang Cheng, Tsi S. Huang, Yung Ching Liu, Chia W. Tang, Chiao Shan Chen, Yao Shen Chen

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Human parechovirus (HPeV) may cause various illnesses; however, technologists and clinicians often overlook it. This study, designed to detect HPeV in 3124 clinical specimens from 2849 patients between January and August 2007, presents the first report of HPeVs confirmed by RNA sequences in Taiwan. Reverse transcriptase polymerase chain reaction (RT-PCR) and phylogenetic tree analysis identified the isolates as HPeV1 (n = 5), HPeV3 (n = 1), and HPeV4 (n = 2) from 6 children. Although the prevalence is low, HPeVs do cause significant clinical manifestations in children. Phylogenetic analysis has separated the 8 HPeV1 strains as a new lineage from the prototype strain (L02971) in evolutionary transition. Clinicians and technologists should have a high index of suspicion and apply RT-PCR for identification when presented with slower Enterovirus-like cytopathic effect (CPE) in cell cultures and negative or equivocal results of staining with indirect immunofluorescence assay, particularly if the CPE is larger, smoother, and more refractive and relatively slow.

Original languageEnglish
Pages (from-to)254-260
Number of pages7
JournalDiagnostic Microbiology and Infectious Disease
Volume65
Issue number3
DOIs
Publication statusPublished - Nov 2009

Keywords

  • Children
  • Cytopathic effect
  • Human parechovirus
  • Pediatrics
  • RT-PCR

ASJC Scopus subject areas

  • Infectious Diseases
  • Microbiology (medical)

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