Abstract
Human parechovirus (HPeV) may cause various illnesses; however, technologists and clinicians often overlook it. This study, designed to detect HPeV in 3124 clinical specimens from 2849 patients between January and August 2007, presents the first report of HPeVs confirmed by RNA sequences in Taiwan. Reverse transcriptase polymerase chain reaction (RT-PCR) and phylogenetic tree analysis identified the isolates as HPeV1 (n = 5), HPeV3 (n = 1), and HPeV4 (n = 2) from 6 children. Although the prevalence is low, HPeVs do cause significant clinical manifestations in children. Phylogenetic analysis has separated the 8 HPeV1 strains as a new lineage from the prototype strain (L02971) in evolutionary transition. Clinicians and technologists should have a high index of suspicion and apply RT-PCR for identification when presented with slower Enterovirus-like cytopathic effect (CPE) in cell cultures and negative or equivocal results of staining with indirect immunofluorescence assay, particularly if the CPE is larger, smoother, and more refractive and relatively slow.
| Original language | English |
|---|---|
| Pages (from-to) | 254-260 |
| Number of pages | 7 |
| Journal | Diagnostic Microbiology and Infectious Disease |
| Volume | 65 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Nov 2009 |
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Keywords
- Children
- Cytopathic effect
- Human parechovirus
- Pediatrics
- RT-PCR
ASJC Scopus subject areas
- Infectious Diseases
- Microbiology (medical)
Cite this
Detection and identification of human parechoviruses from clinical specimens. / Chen, Bao Chen; Cheng, Ming Fang; Huang, Tsi S.; Liu, Yung Ching; Tang, Chia W.; Chen, Chiao Shan; Chen, Yao Shen.
In: Diagnostic Microbiology and Infectious Disease, Vol. 65, No. 3, 11.2009, p. 254-260.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Detection and identification of human parechoviruses from clinical specimens
AU - Chen, Bao Chen
AU - Cheng, Ming Fang
AU - Huang, Tsi S.
AU - Liu, Yung Ching
AU - Tang, Chia W.
AU - Chen, Chiao Shan
AU - Chen, Yao Shen
PY - 2009/11
Y1 - 2009/11
N2 - Human parechovirus (HPeV) may cause various illnesses; however, technologists and clinicians often overlook it. This study, designed to detect HPeV in 3124 clinical specimens from 2849 patients between January and August 2007, presents the first report of HPeVs confirmed by RNA sequences in Taiwan. Reverse transcriptase polymerase chain reaction (RT-PCR) and phylogenetic tree analysis identified the isolates as HPeV1 (n = 5), HPeV3 (n = 1), and HPeV4 (n = 2) from 6 children. Although the prevalence is low, HPeVs do cause significant clinical manifestations in children. Phylogenetic analysis has separated the 8 HPeV1 strains as a new lineage from the prototype strain (L02971) in evolutionary transition. Clinicians and technologists should have a high index of suspicion and apply RT-PCR for identification when presented with slower Enterovirus-like cytopathic effect (CPE) in cell cultures and negative or equivocal results of staining with indirect immunofluorescence assay, particularly if the CPE is larger, smoother, and more refractive and relatively slow.
AB - Human parechovirus (HPeV) may cause various illnesses; however, technologists and clinicians often overlook it. This study, designed to detect HPeV in 3124 clinical specimens from 2849 patients between January and August 2007, presents the first report of HPeVs confirmed by RNA sequences in Taiwan. Reverse transcriptase polymerase chain reaction (RT-PCR) and phylogenetic tree analysis identified the isolates as HPeV1 (n = 5), HPeV3 (n = 1), and HPeV4 (n = 2) from 6 children. Although the prevalence is low, HPeVs do cause significant clinical manifestations in children. Phylogenetic analysis has separated the 8 HPeV1 strains as a new lineage from the prototype strain (L02971) in evolutionary transition. Clinicians and technologists should have a high index of suspicion and apply RT-PCR for identification when presented with slower Enterovirus-like cytopathic effect (CPE) in cell cultures and negative or equivocal results of staining with indirect immunofluorescence assay, particularly if the CPE is larger, smoother, and more refractive and relatively slow.
KW - Children
KW - Cytopathic effect
KW - Human parechovirus
KW - Pediatrics
KW - RT-PCR
UR - http://www.scopus.com/inward/record.url?scp=71749116805&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=71749116805&partnerID=8YFLogxK
U2 - 10.1016/j.diagmicrobio.2009.07.014
DO - 10.1016/j.diagmicrobio.2009.07.014
M3 - Article
VL - 65
SP - 254
EP - 260
JO - Diagnostic Microbiology and Infectious Disease
JF - Diagnostic Microbiology and Infectious Disease
SN - 0732-8893
IS - 3
ER -