Detection and identification of human parechoviruses from clinical specimens

Bao Chen Chen, Ming Fang Cheng, Tsi S. Huang, Yung Ching Liu, Chia W. Tang, Chiao Shan Chen, Yao Shen Chen

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Human parechovirus (HPeV) may cause various illnesses; however, technologists and clinicians often overlook it. This study, designed to detect HPeV in 3124 clinical specimens from 2849 patients between January and August 2007, presents the first report of HPeVs confirmed by RNA sequences in Taiwan. Reverse transcriptase polymerase chain reaction (RT-PCR) and phylogenetic tree analysis identified the isolates as HPeV1 (n = 5), HPeV3 (n = 1), and HPeV4 (n = 2) from 6 children. Although the prevalence is low, HPeVs do cause significant clinical manifestations in children. Phylogenetic analysis has separated the 8 HPeV1 strains as a new lineage from the prototype strain (L02971) in evolutionary transition. Clinicians and technologists should have a high index of suspicion and apply RT-PCR for identification when presented with slower Enterovirus-like cytopathic effect (CPE) in cell cultures and negative or equivocal results of staining with indirect immunofluorescence assay, particularly if the CPE is larger, smoother, and more refractive and relatively slow.

Original languageEnglish
Pages (from-to)254-260
Number of pages7
JournalDiagnostic Microbiology and Infectious Disease
Volume65
Issue number3
DOIs
Publication statusPublished - Nov 2009

Fingerprint

Parechovirus
Reverse Transcriptase Polymerase Chain Reaction
Enterovirus
Indirect Fluorescent Antibody Technique
Taiwan
Cell Culture Techniques
Staining and Labeling

Keywords

  • Children
  • Cytopathic effect
  • Human parechovirus
  • Pediatrics
  • RT-PCR

ASJC Scopus subject areas

  • Infectious Diseases
  • Microbiology (medical)

Cite this

Detection and identification of human parechoviruses from clinical specimens. / Chen, Bao Chen; Cheng, Ming Fang; Huang, Tsi S.; Liu, Yung Ching; Tang, Chia W.; Chen, Chiao Shan; Chen, Yao Shen.

In: Diagnostic Microbiology and Infectious Disease, Vol. 65, No. 3, 11.2009, p. 254-260.

Research output: Contribution to journalArticle

Chen, Bao Chen ; Cheng, Ming Fang ; Huang, Tsi S. ; Liu, Yung Ching ; Tang, Chia W. ; Chen, Chiao Shan ; Chen, Yao Shen. / Detection and identification of human parechoviruses from clinical specimens. In: Diagnostic Microbiology and Infectious Disease. 2009 ; Vol. 65, No. 3. pp. 254-260.
@article{ec949daef37f437b99f5ac89637a100e,
title = "Detection and identification of human parechoviruses from clinical specimens",
abstract = "Human parechovirus (HPeV) may cause various illnesses; however, technologists and clinicians often overlook it. This study, designed to detect HPeV in 3124 clinical specimens from 2849 patients between January and August 2007, presents the first report of HPeVs confirmed by RNA sequences in Taiwan. Reverse transcriptase polymerase chain reaction (RT-PCR) and phylogenetic tree analysis identified the isolates as HPeV1 (n = 5), HPeV3 (n = 1), and HPeV4 (n = 2) from 6 children. Although the prevalence is low, HPeVs do cause significant clinical manifestations in children. Phylogenetic analysis has separated the 8 HPeV1 strains as a new lineage from the prototype strain (L02971) in evolutionary transition. Clinicians and technologists should have a high index of suspicion and apply RT-PCR for identification when presented with slower Enterovirus-like cytopathic effect (CPE) in cell cultures and negative or equivocal results of staining with indirect immunofluorescence assay, particularly if the CPE is larger, smoother, and more refractive and relatively slow.",
keywords = "Children, Cytopathic effect, Human parechovirus, Pediatrics, RT-PCR",
author = "Chen, {Bao Chen} and Cheng, {Ming Fang} and Huang, {Tsi S.} and Liu, {Yung Ching} and Tang, {Chia W.} and Chen, {Chiao Shan} and Chen, {Yao Shen}",
year = "2009",
month = "11",
doi = "10.1016/j.diagmicrobio.2009.07.014",
language = "English",
volume = "65",
pages = "254--260",
journal = "Diagnostic Microbiology and Infectious Disease",
issn = "0732-8893",
publisher = "Elsevier Inc.",
number = "3",

}

TY - JOUR

T1 - Detection and identification of human parechoviruses from clinical specimens

AU - Chen, Bao Chen

AU - Cheng, Ming Fang

AU - Huang, Tsi S.

AU - Liu, Yung Ching

AU - Tang, Chia W.

AU - Chen, Chiao Shan

AU - Chen, Yao Shen

PY - 2009/11

Y1 - 2009/11

N2 - Human parechovirus (HPeV) may cause various illnesses; however, technologists and clinicians often overlook it. This study, designed to detect HPeV in 3124 clinical specimens from 2849 patients between January and August 2007, presents the first report of HPeVs confirmed by RNA sequences in Taiwan. Reverse transcriptase polymerase chain reaction (RT-PCR) and phylogenetic tree analysis identified the isolates as HPeV1 (n = 5), HPeV3 (n = 1), and HPeV4 (n = 2) from 6 children. Although the prevalence is low, HPeVs do cause significant clinical manifestations in children. Phylogenetic analysis has separated the 8 HPeV1 strains as a new lineage from the prototype strain (L02971) in evolutionary transition. Clinicians and technologists should have a high index of suspicion and apply RT-PCR for identification when presented with slower Enterovirus-like cytopathic effect (CPE) in cell cultures and negative or equivocal results of staining with indirect immunofluorescence assay, particularly if the CPE is larger, smoother, and more refractive and relatively slow.

AB - Human parechovirus (HPeV) may cause various illnesses; however, technologists and clinicians often overlook it. This study, designed to detect HPeV in 3124 clinical specimens from 2849 patients between January and August 2007, presents the first report of HPeVs confirmed by RNA sequences in Taiwan. Reverse transcriptase polymerase chain reaction (RT-PCR) and phylogenetic tree analysis identified the isolates as HPeV1 (n = 5), HPeV3 (n = 1), and HPeV4 (n = 2) from 6 children. Although the prevalence is low, HPeVs do cause significant clinical manifestations in children. Phylogenetic analysis has separated the 8 HPeV1 strains as a new lineage from the prototype strain (L02971) in evolutionary transition. Clinicians and technologists should have a high index of suspicion and apply RT-PCR for identification when presented with slower Enterovirus-like cytopathic effect (CPE) in cell cultures and negative or equivocal results of staining with indirect immunofluorescence assay, particularly if the CPE is larger, smoother, and more refractive and relatively slow.

KW - Children

KW - Cytopathic effect

KW - Human parechovirus

KW - Pediatrics

KW - RT-PCR

UR - http://www.scopus.com/inward/record.url?scp=71749116805&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=71749116805&partnerID=8YFLogxK

U2 - 10.1016/j.diagmicrobio.2009.07.014

DO - 10.1016/j.diagmicrobio.2009.07.014

M3 - Article

VL - 65

SP - 254

EP - 260

JO - Diagnostic Microbiology and Infectious Disease

JF - Diagnostic Microbiology and Infectious Disease

SN - 0732-8893

IS - 3

ER -