Detection and identification of human parechoviruses from clinical specimens

Bao Chen Chen; Ming Fang Cheng; Tsi Shu Huang; Yung Ching Liu; Chia Wan Tang; Chiao Shan Chen; Yao Shen Chen

doi:10.1016/j.diagmicrobio.2009.07.014

Detection and identification of human parechoviruses from clinical specimens

Bao Chen Chen, Ming Fang Cheng, Tsi Shu Huang, Yung Ching Liu, Chia Wan Tang, Chiao Shan Chen, Yao Shen Chen

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

Human parechovirus (HPeV) may cause various illnesses; however, technologists and clinicians often overlook it. This study, designed to detect HPeV in 3124 clinical specimens from 2849 patients between January and August 2007, presents the first report of HPeVs confirmed by RNA sequences in Taiwan. Reverse transcriptase polymerase chain reaction (RT-PCR) and phylogenetic tree analysis identified the isolates as HPeV1 (n = 5), HPeV3 (n = 1), and HPeV4 (n = 2) from 6 children. Although the prevalence is low, HPeVs do cause significant clinical manifestations in children. Phylogenetic analysis has separated the 8 HPeV1 strains as a new lineage from the prototype strain (L02971) in evolutionary transition. Clinicians and technologists should have a high index of suspicion and apply RT-PCR for identification when presented with slower Enterovirus-like cytopathic effect (CPE) in cell cultures and negative or equivocal results of staining with indirect immunofluorescence assay, particularly if the CPE is larger, smoother, and more refractive and relatively slow.

Original language	English
Pages (from-to)	254-260
Number of pages	7
Journal	Diagnostic Microbiology and Infectious Disease
Volume	65
Issue number	3
DOIs	https://doi.org/10.1016/j.diagmicrobio.2009.07.014
Publication status	Published - Nov 2009

Keywords

Children
Cytopathic effect
Human parechovirus
Pediatrics
RT-PCR

ASJC Scopus subject areas

Microbiology (medical)
Infectious Diseases

UN SDGs

This output contributes to the following Sustainable Development Goal(s)

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10.1016/j.diagmicrobio.2009.07.014

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title = "Detection and identification of human parechoviruses from clinical specimens",

abstract = "Human parechovirus (HPeV) may cause various illnesses; however, technologists and clinicians often overlook it. This study, designed to detect HPeV in 3124 clinical specimens from 2849 patients between January and August 2007, presents the first report of HPeVs confirmed by RNA sequences in Taiwan. Reverse transcriptase polymerase chain reaction (RT-PCR) and phylogenetic tree analysis identified the isolates as HPeV1 (n = 5), HPeV3 (n = 1), and HPeV4 (n = 2) from 6 children. Although the prevalence is low, HPeVs do cause significant clinical manifestations in children. Phylogenetic analysis has separated the 8 HPeV1 strains as a new lineage from the prototype strain (L02971) in evolutionary transition. Clinicians and technologists should have a high index of suspicion and apply RT-PCR for identification when presented with slower Enterovirus-like cytopathic effect (CPE) in cell cultures and negative or equivocal results of staining with indirect immunofluorescence assay, particularly if the CPE is larger, smoother, and more refractive and relatively slow.",

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author = "Chen, {Bao Chen} and Cheng, {Ming Fang} and Huang, {Tsi Shu} and Liu, {Yung Ching} and Tang, {Chia Wan} and Chen, {Chiao Shan} and Chen, {Yao Shen}",

note = "Funding Information: The authors thank Prof Cheng T. Cho (University of Kansas Medical Center, Kansas City, Kansas) for his review of the manuscript. This work was supported by a grant (VGHKS 97-069) from the Veterans General Hospital-Kaohsiung, Kaohsiung, Taiwan. Copyright: Copyright 2010 Elsevier B.V., All rights reserved.",

year = "2009",

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doi = "10.1016/j.diagmicrobio.2009.07.014",

language = "English",

volume = "65",

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AU - Chen, Bao Chen

AU - Cheng, Ming Fang

AU - Huang, Tsi Shu

AU - Liu, Yung Ching

AU - Tang, Chia Wan

AU - Chen, Chiao Shan

AU - Chen, Yao Shen

N1 - Funding Information: The authors thank Prof Cheng T. Cho (University of Kansas Medical Center, Kansas City, Kansas) for his review of the manuscript. This work was supported by a grant (VGHKS 97-069) from the Veterans General Hospital-Kaohsiung, Kaohsiung, Taiwan. Copyright: Copyright 2010 Elsevier B.V., All rights reserved.

PY - 2009/11

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N2 - Human parechovirus (HPeV) may cause various illnesses; however, technologists and clinicians often overlook it. This study, designed to detect HPeV in 3124 clinical specimens from 2849 patients between January and August 2007, presents the first report of HPeVs confirmed by RNA sequences in Taiwan. Reverse transcriptase polymerase chain reaction (RT-PCR) and phylogenetic tree analysis identified the isolates as HPeV1 (n = 5), HPeV3 (n = 1), and HPeV4 (n = 2) from 6 children. Although the prevalence is low, HPeVs do cause significant clinical manifestations in children. Phylogenetic analysis has separated the 8 HPeV1 strains as a new lineage from the prototype strain (L02971) in evolutionary transition. Clinicians and technologists should have a high index of suspicion and apply RT-PCR for identification when presented with slower Enterovirus-like cytopathic effect (CPE) in cell cultures and negative or equivocal results of staining with indirect immunofluorescence assay, particularly if the CPE is larger, smoother, and more refractive and relatively slow.

AB - Human parechovirus (HPeV) may cause various illnesses; however, technologists and clinicians often overlook it. This study, designed to detect HPeV in 3124 clinical specimens from 2849 patients between January and August 2007, presents the first report of HPeVs confirmed by RNA sequences in Taiwan. Reverse transcriptase polymerase chain reaction (RT-PCR) and phylogenetic tree analysis identified the isolates as HPeV1 (n = 5), HPeV3 (n = 1), and HPeV4 (n = 2) from 6 children. Although the prevalence is low, HPeVs do cause significant clinical manifestations in children. Phylogenetic analysis has separated the 8 HPeV1 strains as a new lineage from the prototype strain (L02971) in evolutionary transition. Clinicians and technologists should have a high index of suspicion and apply RT-PCR for identification when presented with slower Enterovirus-like cytopathic effect (CPE) in cell cultures and negative or equivocal results of staining with indirect immunofluorescence assay, particularly if the CPE is larger, smoother, and more refractive and relatively slow.

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Detection and identification of human parechoviruses from clinical specimens

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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