Desipramine activated Bcl-2 expression and inhibited lipopolysaccharide- induced apoptosis in hippocampus-derived adult neural stem cells

Yu Yin Huang, Chi Hsien Peng, Yi Ping Yang, Chih Chiau Wu, Wen-Ming Hsu, Hsiao Jung Wang, Kwok Han Chan, Yi Pen Chou, Shih Jen Chen, Yuh Lih Chang

Research output: Contribution to journalArticle

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Abstract

Desipramine (DP) is a tricyclic antidepressant used for treating depression and numerous other psychiatric disorders. Recent studies have shown that DP can promote neurogenesis and improve the survival rate of hippocampal neurons. However, whether DP induces neuroprotection or promotes the differentiation of neural stem cells (NSCs) needs to be elucidated. In this study, we cultured NSCs derived from the hippocampal tissues of adult rats as an in vitro model to evaluate the modulation effect of DP on NSCs. First, we demonstrated that the expression of Bcl-2 mRNA and nestin in 2 μM DP-treated NSCs were up-regulated and detected by real-time reverse transcriptase polymerase chain reaction (RT-PCR). The results of Western blotting and immunofluorescent study confirmed that Bcl-2 protein expression was significantly increased in Day 3 DP-treated NSCs. Using the Bcl-2 small interfering RNA (siRNA) method, our results further showed that DP protects the lipopolysaccharide (LPS)-induced apoptosis in NSCs, in part by activating the expression of Bcl-2. Furthermore, DP treatment significantly inhibited the induction of proinflammatory factor interleukin (IL)-1β, IL-6, and tumor necrosis factor-α in the culture medium of LPS-treated NSCs mediated by Bcl-2 modulation. The results of high performance liquid chromatography coupled to electro-chemical detection further confirmed that DP significantly increased the functional production of serotonin (26 ± 3.5 μM, DP-treated 96 h) and noradrenaline (50 ± 8.9 μM, DP-treated 96 h) in NSCs through activation of the MAPK/ERK pathway and partially mediated by Bcl-2. In conclusion, the present results indicate that DP can increase neuroprotection ability by inhibiting the LPS-induced inflammatory process in NSCs via the modulation of Bcl-2 expression, as confirmed by the siRNA method.

Original languageEnglish
Pages (from-to)61-72
Number of pages12
JournalJournal of Pharmacological Sciences
Volume104
Issue number1
DOIs
Publication statusPublished - May 28 2007
Externally publishedYes

Fingerprint

Adult Stem Cells
Desipramine
Neural Stem Cells
Lipopolysaccharides
Hippocampus
Apoptosis
Small Interfering RNA
Nestin
MAP Kinase Signaling System
Tricyclic Antidepressive Agents
Neurogenesis
Reverse Transcriptase Polymerase Chain Reaction
Interleukin-1
Psychiatry
Culture Media
Real-Time Polymerase Chain Reaction
Interleukin-6
Serotonin
Norepinephrine
Tumor Necrosis Factor-alpha

Keywords

  • Desipramine
  • Neural stem cell
  • Real-time RT-PCR
  • Small interfering rna (siRNA)

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology

Cite this

Desipramine activated Bcl-2 expression and inhibited lipopolysaccharide- induced apoptosis in hippocampus-derived adult neural stem cells. / Huang, Yu Yin; Peng, Chi Hsien; Yang, Yi Ping; Wu, Chih Chiau; Hsu, Wen-Ming; Wang, Hsiao Jung; Chan, Kwok Han; Chou, Yi Pen; Chen, Shih Jen; Chang, Yuh Lih.

In: Journal of Pharmacological Sciences, Vol. 104, No. 1, 28.05.2007, p. 61-72.

Research output: Contribution to journalArticle

Huang, Yu Yin ; Peng, Chi Hsien ; Yang, Yi Ping ; Wu, Chih Chiau ; Hsu, Wen-Ming ; Wang, Hsiao Jung ; Chan, Kwok Han ; Chou, Yi Pen ; Chen, Shih Jen ; Chang, Yuh Lih. / Desipramine activated Bcl-2 expression and inhibited lipopolysaccharide- induced apoptosis in hippocampus-derived adult neural stem cells. In: Journal of Pharmacological Sciences. 2007 ; Vol. 104, No. 1. pp. 61-72.
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AU - Hsu, Wen-Ming

AU - Wang, Hsiao Jung

AU - Chan, Kwok Han

AU - Chou, Yi Pen

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AU - Chang, Yuh Lih

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