Since biocomputing plays an essential role in the biological and medical fields, the catalytic features of enzymes can be utilized in medical applications as biosensor. In this study, we tried to build an enzymatic system as biosensor equipment application to measure blood sugar with sequential reactions of enzymes, which express logic output signals with latent fluorophore as a reporter. The expected biosensor will analyze three molecules sucrose, maltose and ATP existing in human blood, by adding some blood into the system consisted of various enzymes. Sucrose and Maltose will be decomposed into glucose; moreover, glucose and ATP have critical influence of glycolysis which shows one crucial reaction of human metabolism. The output signal was visualized by high throughput fluorescence that originated from the long-wavelength latent fluorogenic substrate-Salicylate hydroxylase (SHL) reaction. The experiment results of this research have showed the possibility to determine the combinations of the three important components, by setting the concentration of each input molecules to specific threshold values. Further researches are required to find out the relationship between the specific concentration and measure glucose results, especially in the medical field.