Abstract

Activated microglial cells are present in dengue virus (DENV)-infected brains; however, the possible effects of DENV on microglia remain unclear. Here, we demonstrated DENV caused infection, including viral entry, RNA replication, viral protein expression, and virus release, in the murine microglial cell line BV2. DENV infection caused an increase in the formation of the multipolar phenotype in vitro and in vivo without affecting cell growth and cytotoxicity. DENV infection considerably increased cell motility and disrupting either actin filaments or clathrin retarded such effect. Increase in cell migration was only occurred by DENV infection following a clathrin-regulated endocytosis of DENV entry. Ultravioletinactivated DENV did not affect cell migration, and pharmacologically blocking toll-like receptor (TLR) 3 and TLR3-related signaling pathways reduced the DENV-induced increase in cell migration. These results demonstrate an advanced effect of DENV infection on microglial migration via a mechanism involving viral entry, RNA release, and TLR3 signal activation.

Original languageEnglish
Article number91
JournalScientific Reports
Volume7
Issue number1
DOIs
Publication statusPublished - Jan 1 2017

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Dengue Virus
Virus Diseases
Cell Movement
Clathrin
Viral RNA
Toll-Like Receptor 3
Virus Release
Virus Internalization
Microglia
Viral Proteins
Endocytosis
Actin Cytoskeleton
Phenotype
Cell Line

ASJC Scopus subject areas

  • General

Cite this

Dengue virus infection increases microglial cell migration. / Jhan, Ming Kai; Tsai, Tsung Ting; Chen, Chia Ling; Tsai, Cheng Chieh; Cheng, Yi Lin; Lee, Yi Chao; Ko, Chiung Yuan; Lin, Yee Shin; Chang, Chih Peng; Lin, Liang Tzung; Lin, Chiou Feng.

In: Scientific Reports, Vol. 7, No. 1, 91, 01.01.2017.

Research output: Contribution to journalArticle

Jhan, Ming Kai ; Tsai, Tsung Ting ; Chen, Chia Ling ; Tsai, Cheng Chieh ; Cheng, Yi Lin ; Lee, Yi Chao ; Ko, Chiung Yuan ; Lin, Yee Shin ; Chang, Chih Peng ; Lin, Liang Tzung ; Lin, Chiou Feng. / Dengue virus infection increases microglial cell migration. In: Scientific Reports. 2017 ; Vol. 7, No. 1.
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AU - Ko, Chiung Yuan

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