Dendritic cells respond to nasopharygeal carcinoma cells through annexin A2-recognizing DC-SIGN

Pin-Zhir Chao, Ming-Shium Hsieh, Chao-Wen Cheng, Tin Jui Hsu, Yun Tien Lin, Chang Hao Lai, Chen Chung Liao, Wei-Yu Chen, Ting-Kai Leung, Fei-Peng Lee, Yung-Feng Lin, Chien-Ho Chen

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Dendritic cells (DCs) play an essential role in immunity and are used in cancer immunotherapy. However, these cells can be tuned by tumors with immunosuppressive responses. DC-specific intercellular adhesion molecule 3-Grabbing Nonintegrin (DC-SIGN), a C-type lectin expressed on DCs, recognizes certain carbohydrate structures which can be found on cancer cells. Nasopharyngeal carcinoma (NPC) is an epithelial cell-derived malignant tumor, in which immune response remains unclear. This research is to reveal the molecular link on NPC cells that induces the immunosuppressive responses in DCs. In this article, we report identification of annexin A2 (ANXA2) on NPC cells as a ligand for DC-SIGN on DCs. N-linked mannoserich glycan on ANXA2 may mediate the interaction. ANXA2 was abundantly expressed in NPC, and knockdown of ANXA2 suppressed NPC xenograft in mice, suggesting a crucial role of ANXA2 in NPC growth. Interaction with NPC cells caused DC-SIGN activation in DCs. Consequently DC maturation and the proinflammatory interleukin (IL)-12 production were inhibited, and the immunosuppressive IL-10 production was promoted. Blockage of either DC-SIGN or ANXA2 eliminated the production of IL- 10 from DCs. This report suggests that suppression of ANXA2 at its expression or glycosylation on NPC may improve DC-mediated immunotherapy for the tumor.

Original languageEnglish
Pages (from-to)159-170
Number of pages12
JournalOncotarget
Volume6
Issue number1
Publication statusPublished - 2015

Fingerprint

Annexins
Dendritic Cells
Annexin A2
Carcinoma
Immunosuppressive Agents
Neoplasms
Interleukin-10
Immunotherapy
C-Type Lectins
Nasopharyngeal carcinoma
Cell Adhesion Molecules
Interleukin-12
Glycosylation
Heterografts
Polysaccharides
Immunity

Keywords

  • Annexin A2
  • DC-SIGN
  • Dendritic cell
  • IL-10
  • Immunosuppression
  • NPC

ASJC Scopus subject areas

  • Oncology

Cite this

Chao, P-Z., Hsieh, M-S., Cheng, C-W., Hsu, T. J., Lin, Y. T., Lai, C. H., ... Chen, C-H. (2015). Dendritic cells respond to nasopharygeal carcinoma cells through annexin A2-recognizing DC-SIGN. Oncotarget, 6(1), 159-170.

Dendritic cells respond to nasopharygeal carcinoma cells through annexin A2-recognizing DC-SIGN. / Chao, Pin-Zhir; Hsieh, Ming-Shium; Cheng, Chao-Wen; Hsu, Tin Jui; Lin, Yun Tien; Lai, Chang Hao; Liao, Chen Chung; Chen, Wei-Yu; Leung, Ting-Kai; Lee, Fei-Peng; Lin, Yung-Feng; Chen, Chien-Ho.

In: Oncotarget, Vol. 6, No. 1, 2015, p. 159-170.

Research output: Contribution to journalArticle

Chao, P-Z, Hsieh, M-S, Cheng, C-W, Hsu, TJ, Lin, YT, Lai, CH, Liao, CC, Chen, W-Y, Leung, T-K, Lee, F-P, Lin, Y-F & Chen, C-H 2015, 'Dendritic cells respond to nasopharygeal carcinoma cells through annexin A2-recognizing DC-SIGN', Oncotarget, vol. 6, no. 1, pp. 159-170.
Chao, Pin-Zhir ; Hsieh, Ming-Shium ; Cheng, Chao-Wen ; Hsu, Tin Jui ; Lin, Yun Tien ; Lai, Chang Hao ; Liao, Chen Chung ; Chen, Wei-Yu ; Leung, Ting-Kai ; Lee, Fei-Peng ; Lin, Yung-Feng ; Chen, Chien-Ho. / Dendritic cells respond to nasopharygeal carcinoma cells through annexin A2-recognizing DC-SIGN. In: Oncotarget. 2015 ; Vol. 6, No. 1. pp. 159-170.
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