Dendritic cells modulated by cytokine-expressing adenoviruses alleviate eosinophilia and airway hyperresponsiveness in an animal model of asthma

Yi Ling Ye, Yueh Lun Lee, Zen Jai Chuang, Huai Jean Lai, Chun Chi Chen, Mi Hua Tao, Bor Luen Chiang

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Background It has been found that T H1-related cytokines can decrease the accumulation of eosinophils in lung tissue and relieve airway constriction. Objective Dendritic cells (DCs) have been found to prime naive T-helper cells efficiently. In this study, DCs infected with T H1 cytokine-expressing adenovirus can be used to induce antigen-specific T H1 cells for treatment in an animal model of asthma. Methods Cytokine gene-modulated DCs pulsed with ovalbumin antigen (OVA) were injected intravenously into naive mice 1 week before sensitization with OVA antigen. The mice were then monitored for OVA-specific IgE, airway inflammatory cell infiltration, and airway hyperresponsiveness in the study. Results Significant levels of IL-12 or IL-18 were expressed by Ad-IL-12 or Ad-IL-18 infected, bone marrow-derived DCs. Ad-IL-12 and Ad-IL-18 co-infected DCs effectively, decreasing sera anti-OVA IgE antibody levels, lung eosinophilia, and airway hyperresponsiveness. Conclusion We concluded that DCs modulated by T H1-prone cytokine-expressing adenoviruses can alleviate T H2-type airway inflammation in a murine model and can provide possible therapeutic application for DCs in asthma.

Original languageEnglish
Pages (from-to)88-96
Number of pages9
JournalJournal of Allergy and Clinical Immunology
Volume114
Issue number1
DOIs
Publication statusPublished - Jul 2004
Externally publishedYes

Keywords

  • AHR
  • Airway hyperresponsiveness
  • Asthma animal model
  • BAL
  • Bronchoalveolar lavage
  • cytokine expressing adenovirus
  • DCs
  • dendritic cell
  • Dendritic cells
  • GFP
  • Green fluorescence protein
  • MOI
  • Multiplicity of infection
  • OVA
  • Ovalbumin

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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