Dehydroxyhispolon methyl ether, a hispolon derivative, inhibits WNT/β-catenin signaling to elicit human colorectal carcinoma cell apoptosis

Hueng Chuen Fan, Ya Chu Hsieh, Li Hsuan Li, Ching Chin Chang, Karolína Janoušková, Modukuri V. Ramani, Gottumukkala V. Subbaraju, Kur Ta Cheng, Chia Che Chang

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Colorectal cancer (CRC) is the fourth leading cause of cancer mortality worldwide. Aberrant activation of WNT/β-catenin signaling present in the vast majority of CRC cases is indispensable for CRC initiation and progression, and thus is a promising target for CRC therapeutics. Hispolon is a fungal-derived polyphenol with a pronounced anticancer effect. Several hispolon derivatives, including dehydroxyhispolon methyl ether (DHME), have been chemically synthesized for developing lead molecules with stronger anticancer activity. Herein, a DHME-elicited anti-CRC effect with the underlying mechanism is reported for the first time. Specifically, DHME was found to be more cytotoxic than hispolon against a panel of human CRC cell lines, while exerting limited toxicity to normal human colon cell line CCD 841 CoN. Additionally, the cytotoxic effect of DHME appeared to rely on inducing apoptosis. This notion was evidenced by DHME-elicited upregulation of poly (ADP-ribose) polymerase (PARP) cleavage and a cell population positively stained by annexin V, alongside the downregulation of antiapoptotic B-cell lymphoma 2 (BCL-2), whereas the blockade of apoptosis by the pan-caspase inhibitor z-VAD-fmk attenuated DHME-induced cytotoxicity. Further mechanistic inquiry revealed the inhibitory action of DHME on β-catenin-mediated, T-cell factor (TCF)-dependent transcription activity, suggesting that DHME thwarted the aberrantly active WNT/β-catenin signaling in CRC cells. Notably, ectopic expression of a dominant–active β-catenin mutant (∆N90-β-catenin) abolished DHME-induced apoptosis while also restoring BCL-2 expression. Collectively, we identified DHME as a selective proapoptotic agent against CRC cells, exerting more potent cytotoxicity than hispolon, and provoking CRC cell apoptosis via suppression of the WNT/β-catenin signaling axis.

Original languageEnglish
Article number8839
Pages (from-to)1-14
Number of pages14
JournalInternational journal of molecular sciences
Volume21
Issue number22
DOIs
Publication statusPublished - Nov 2 2020

Keywords

  • Colorectal cancer
  • Dehydroxyhispolon methyl ether
  • Hispolon
  • Hispolon derivatives
  • Phellinus linteus
  • WNT/β-catenin

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Fingerprint

Dive into the research topics of 'Dehydroxyhispolon methyl ether, a hispolon derivative, inhibits WNT/β-catenin signaling to elicit human colorectal carcinoma cell apoptosis'. Together they form a unique fingerprint.

Cite this