Deformability of mice erythrocytes measured by oscillatory optical tweezers

Yu Shan Huang, Chia Lun Yeh, Guan Bo Liao, Yi Fan Chen, Ting Fen Tsai, Arthur Chiou

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

We used one-dimensional oscillatory optical tweezers in a discrete scanning (or jumping) mode to trap and stretch individual mice erythrocytes and measured their deformation as a function of the jumping distance of the oscillatory trapping beam. In general, we observed that the length of the long axis of red blood cells (RBCs) decreased slightly at small jumping distance and then increased after the jumping distance exceeded a threshold value on the order of a few microns, which is consistent with a recent theoretical prediction. The deformability of three types of mice RBCs, namely the wide-type mice (serving as the control group), old mice, and gene knockout mice were measured and compared. Statistical analysis of their deformability reveals that the RBCs of old mice can be distinguished from those of knockout mice even though these two types of mice exhibit many similar aging-like features.

Original languageEnglish
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
Volume7038
DOIs
Publication statusPublished - 2008
Externally publishedYes
EventOptical Trapping and Optical Micromanipulation V - San Diego, CA, United States
Duration: Aug 10 2008Aug 13 2008

Other

OtherOptical Trapping and Optical Micromanipulation V
CountryUnited States
CitySan Diego, CA
Period8/10/088/13/08

Fingerprint

Optical tweezers
Optical Tweezers
Erythrocyte
erythrocytes
Formability
mice
Mouse
Blood
knockout mice
Cells
Red Blood Cells
Statistical methods
Genes
Aging of materials
Scanning
genes
statistical analysis
Stretch
Threshold Value
Trapping

Keywords

  • Erythrocyte
  • Gene knockout
  • Oscillatory optical tweezers

ASJC Scopus subject areas

  • Applied Mathematics
  • Computer Science Applications
  • Electrical and Electronic Engineering
  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics

Cite this

Huang, Y. S., Yeh, C. L., Liao, G. B., Chen, Y. F., Tsai, T. F., & Chiou, A. (2008). Deformability of mice erythrocytes measured by oscillatory optical tweezers. In Proceedings of SPIE - The International Society for Optical Engineering (Vol. 7038). [703809] https://doi.org/10.1117/12.794690

Deformability of mice erythrocytes measured by oscillatory optical tweezers. / Huang, Yu Shan; Yeh, Chia Lun; Liao, Guan Bo; Chen, Yi Fan; Tsai, Ting Fen; Chiou, Arthur.

Proceedings of SPIE - The International Society for Optical Engineering. Vol. 7038 2008. 703809.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Huang, YS, Yeh, CL, Liao, GB, Chen, YF, Tsai, TF & Chiou, A 2008, Deformability of mice erythrocytes measured by oscillatory optical tweezers. in Proceedings of SPIE - The International Society for Optical Engineering. vol. 7038, 703809, Optical Trapping and Optical Micromanipulation V, San Diego, CA, United States, 8/10/08. https://doi.org/10.1117/12.794690
Huang YS, Yeh CL, Liao GB, Chen YF, Tsai TF, Chiou A. Deformability of mice erythrocytes measured by oscillatory optical tweezers. In Proceedings of SPIE - The International Society for Optical Engineering. Vol. 7038. 2008. 703809 https://doi.org/10.1117/12.794690
Huang, Yu Shan ; Yeh, Chia Lun ; Liao, Guan Bo ; Chen, Yi Fan ; Tsai, Ting Fen ; Chiou, Arthur. / Deformability of mice erythrocytes measured by oscillatory optical tweezers. Proceedings of SPIE - The International Society for Optical Engineering. Vol. 7038 2008.
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