Defensin protein from sweet potato (Ipomoea batatas [L.] Lam 'Tainong 57') storage roots exhibits antioxidant activities in vitro and ex vivo

Guan Jhong Huang, Jeng Shyan Deng, Hsien Jung Chen, Shyh Shyun Huang, Jung Chun Liao, Wen Chi Hou, Yaw Huei Lin

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

This study was designed to investigate the antioxidant activities of sweet potato defensin (SPD1) in vitro and ex vivo. Antioxidant status [2,2′-azinobis[3-ethylbenzthiazoline-6-sulphonic acid (ABTS) assay], scavenging activity against DPPH (1,1-dipheny-2-picrylhydrazyl) radical method, reducing power method, Fe2+-chelating ability, FTC (ferric thiocyanate) method, and protection of calf thymus DNA against hydroxyl radical-induced damage were studied in vitro. The ex vivo experiments revealed that SPD1 could decrease the production of intracellular peroxide in HepG2 cells. Four peptides, namely GFR, GPCSR, CFCTKPC and MCESASSK for testing antioxidative activity, were synthesized according to tryptic hydrolysis simulation. In the TEAC assay CFCTKPC performed the best (13.5 ± 0.3 μmol TE/g dw), even better than reduced glutathione (7.3 ± 0.2 μmol TE/g dw). In the DPPH radical assay (%), [IC50 (μM) (the concentration required for scavenging 50% activity)] CFCTKPC again had the highest antioxidant activity (IC50 is 11.3 ± 3.2 μM) even better than reduced glutathione (IC50 is 74.3 ± 2.4 μM). In the lipid peroxidation assay, once again CFCTKPC performed the best, with an IC50 value of 0.5 ± 0.0 μM better than reduced glutathione (1.2 ± 0.1 μM). These findings mean that cysteine residue is most important in antioxidant activities. It was suggested that SPD1 might contribute its antioxidant activities against hydroxyl and peroxyl radicals.

Original languageEnglish
Pages (from-to)861-867
Number of pages7
JournalFood Chemistry
Volume135
Issue number3
DOIs
Publication statusPublished - Dec 1 2012

Fingerprint

Ipomoea batatas
Defensins
sweet potatoes
Antioxidants
antioxidant activity
Inhibitory Concentration 50
inhibitory concentration 50
Assays
Glutathione
glutathione
Scavenging
assays
Proteins
proteins
Hydroxyl Radical
thiocyanates
Sulfonic Acids
sulfonic acid
Peroxides
Hep G2 Cells

Keywords

  • Antioxidant
  • Defensin
  • Sweet potato

ASJC Scopus subject areas

  • Food Science
  • Analytical Chemistry

Cite this

Defensin protein from sweet potato (Ipomoea batatas [L.] Lam 'Tainong 57') storage roots exhibits antioxidant activities in vitro and ex vivo. / Huang, Guan Jhong; Deng, Jeng Shyan; Chen, Hsien Jung; Huang, Shyh Shyun; Liao, Jung Chun; Hou, Wen Chi; Lin, Yaw Huei.

In: Food Chemistry, Vol. 135, No. 3, 01.12.2012, p. 861-867.

Research output: Contribution to journalArticle

Huang, Guan Jhong ; Deng, Jeng Shyan ; Chen, Hsien Jung ; Huang, Shyh Shyun ; Liao, Jung Chun ; Hou, Wen Chi ; Lin, Yaw Huei. / Defensin protein from sweet potato (Ipomoea batatas [L.] Lam 'Tainong 57') storage roots exhibits antioxidant activities in vitro and ex vivo. In: Food Chemistry. 2012 ; Vol. 135, No. 3. pp. 861-867.
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AU - Hou, Wen Chi

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